biosynthesis regulation of sialyl lewis X in sera and its participation in immune response

• Project/Area Number: 19790398
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Laboratory medicine
• Research Institution: Toho University
• Principal Investigator: HIGAI Koji Toho University, 薬学部, 講師 (70297711)
• Project Period (FY): 2007 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥3,590,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥390,000); Fiscal Year 2008: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2007: ¥1,900,000 (Direct Cost: ¥1,900,000)
• Keywords: 臨床化学 / sialyl Lewis X / CD94 / NKG2D / Lectin / α2, 3-sialic acid / NK細胞 / Fucosyltransferase VI / promoter / IL-1β / IL-6

Research Abstract

初めに、sLeX合成関連糖転移酵素群のFUT VIの遺伝子発現制御機構を転写レベルで解析し、転写開始点およびHNF-4αがFUT VIの発現に重要であることを明らかにした。つぎに、sLeXの生体内免疫応答への関与を、NK細胞上のCD94およびNKG2Dに着目し、その糖鎖リガンドを明らかにした。
本研究により、NK細胞上のCD94およびNKG2DがsLeX様の糖鎖を認識し、がん細胞の増殖を抑制することが考えられた。

Research Products

• [Journal Article] NKG2D and CD94 bind to multimeric α2, 3-linked N-acetylneuraminic acid (2008)
  • Author(s): Y. Imaizumi, K. Higai, et.al.
  • Journal Title: Biochem. Biophys. Res. Commun 328 Pages: 604-608
  • Peer Reviewed
• [Journal Article] Transcriptional regulation of the fucosyltransferase VI gene in hepatocel lular carcinoma cells (2008)
  • Author(s): Koji Higai, et. al.
  • Journal Title: Glycoconjugate Journal 3 Pages: 225-235
  • Peer Reviewed
• [Presentation] NKG2Dリガンドの解析 (2009)
  • Author(s): Y. Imaizumi, K. Higai, et.al.
  • Organizer: 第129回日本薬学会年会
  • Place of Presentation: Kyoto, Japan
  • Year and Date: 2009-03-25
• [Presentation] NKG2Dリガンドの解析 (2009)
  • Author(s): 今泉雄三、桧貝孝慈、東祐太郎、松本宏治郎
  • Organizer: 第129回日本薬学会年会
  • Place of Presentation: 京都
• [Presentation] NKG2D mediates cytotoxicity against high sialyl Lewis X-expresing cells (2008)
  • Author(s): K. Higai, Y. Imaizumi, et.al.
  • Organizer: Biochemistry and Molecular Biology 2008
  • Place of Presentation: Kobe, Japan
  • Year and Date: 2008-12-12
• [Presentation] NKG2D mediates cytotoxicity against high sialyl Lewis X-expresing cells (2008)
  • Author(s): Higai K., Imaizumi Y., Azuma Y, Matsumoto K
  • Organizer: Biochemistry and Molecular Biology
  • Place of Presentation: Kobe
• [Presentation] NKG2D糖鎖リガンドの解析 (2008)
  • Author(s): 今泉雄三、楠原彬子、桧貝孝慈、東祐太郎、松本宏治郎
  • Organizer: 第128回日本薬学会年会
  • Place of Presentation: 横浜
• [Presentation] Transcriptional regulation of the fucosyltransferase VI gene in hepatocel lular carcinoma cells (2007)
  • Author(s): Koji Higai, et. al.
  • Organizer: Biochemistry and Molecular Biology 2007
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 2007-12-14
• [Presentation] Transcriptional regulation of FUT VI in human hepatocellular carcinoma HepG2 cells (2007)
  • Author(s): Higai K, Miyazaki N, Matsumoto K
  • Organizer: Biochemistry and Molecular biology 2007
  • Place of Presentation: Yokohama
• [Remarks]
  • URL: http://www.phar.toho-u.ac.jp/facilities/open_reserch/15208/theme2.html