Effect of long-term exposure to asbestos on the activating system of NK cells

• Project/Area Number: 19790431
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Public health/Health science
• Research Institution: Kawasaki Medical School
• Principal Investigator: NISHIMURA Yasumitsu Kawasaki Medical School, 医学部, 講師 (90360271)
• Project Period (FY): 2007 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥3,650,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥450,000); Fiscal Year 2008: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000); Fiscal Year 2007: ¥1,700,000 (Direct Cost: ¥1,700,000)
• Keywords: アスベスト / NK細胞 / 悪性中皮腫 / NK細胞活性化受容体 / NKp46 / chrysotile / 免疫学

Research Abstract

抗腫瘍免疫を担うNK細胞機能へのアスベスト曝露の影響について調べた。培養実験と患者NK細胞の機能解析より、アスベスト曝露がNK細胞機能低下に関わることが示された。悪性中皮腫患者末梢血中のNK細胞の細胞傷害性および活性化に関わる受容体NKp46発現量が低下していること、アスベスト曝露下培養後のNK細胞で同じようにNKp46発現低下が見られることが明らかとなった。アスベスト曝露による抗腫瘍免疫抑制作用が示唆される。

Research Products

• [Journal Article] Immunological alterations found in mesothelioma patients and their experimental evidences (2008)
  • Author(s): Miura Y, Nishimura Y, Maeda M, Murakami S, Hayashi H, Fukuoka K, Kishimoto T,Nakano T, Otsuki T
  • Journal Title: Environ Health Prev Med 13 Pages: 55-59
  • Peer Reviewed
• [Journal Article] アスベスト関連疾患の発症機構-悪性中皮腫を中心として- (2008)
  • Author(s): 西村泰光,前田恵,村上周子,熊谷直子,林宏明,大槻剛巳
  • Journal Title: Surgery Frontier 15(2) Pages: 8-13
• [Journal Article] 大気環境汚染による肺疾患.日本臨床別冊 (2008)
  • Author(s): 村上周子,前田恵,林宏明,西村泰光,大槻剛巳
  • Journal Title: 呼吸器症候群(I)-その他の呼吸器疾患を含めて- Pages: 593-596
• [Journal Article] アスベスト関連疾患の発症機構-悪性中皮腫を中心として- (2008)
  • Author(s): 西村泰光
  • Journal Title: Surgery Frontier 15 Pages: 55-59
• [Journal Article] Immunological alterations found in mesothelioma patients and their experimental evidences (2008)
  • Author(s): Yoshie Miura
  • Journal Title: Environmental Health and Preventive Medicine 13 Pages: 55-59
  • Peer Reviewed
• [Journal Article] Immunological changes in mesothelioma patients and their experimental detection (2008)
  • Author(s): Megumi Maeda
  • Journal Title: Clinical Medicine : Circulatory, Respiratory and Pulmonary Medicine 2 Pages: 11-17
  • Peer Reviewed
• [Journal Article] Immunological changes in mesothelioma patients and their experimental detection (2007)
  • Author(s): Maeda M, Miura Y, Nishimura Y, Murakami S, Hayashi H, Kumagai N, Hatayama T, Katoh M, Miyahara N, Yamamoto S, Fukuoka K, KishimotoT, Nakano T, Otsuki T
  • Journal Title: Clin Med : Circ, Resp Pulm Med 2 Pages: 11-17
  • Peer Reviewed
• [Journal Article] Keynote lecture in the 13th Japanese Society of Immunotoxicology (JSIT 2006) ---Pathophysiological Development and Immunotoxicology : what we have found from the researches related to the silica and silicate such as asbestos--- (2007)
  • Author(s): Otsuki T, Miura Y, Maeda M, Hayashi H, Dong M, Nishimura Y
  • Journal Title: Environ Health Prev Med 12(4) Pages: 153-164
  • NAID: 110006386435
  • Peer Reviewed
• [Journal Article] Keynote lecture in the 13th Japanese Society of Immunotoxicology (JSIT 2006) --- Pathophysiological Development and Immunotoxicology: what we have found from the researches related to the silica and silicate such as asbestos--- (2007)
  • Author(s): Otsuki T, Miura Y, Hyodoh F, Maeda M, Hayashi H, Dong M, Katsuyama H, Tomita M, Ueki A, Nishimura Y.
  • Journal Title: Kawasaki Med J 33(3) Pages: 165-179
  • NAID: 110006386435
  • Peer Reviewed
• [Journal Article] Immunological effects of silica and asbestos. (2007)
  • Author(s): Otsuki T, Maeda M, Murakami S, Hayashi H, Miura Y, Kusaka M, Nakano T, Fukuoka K, Kishimoto T, Hyodoh F, Ueki A, Nishimura Y
  • Journal Title: Cell Mol Immnol 4(4) Pages: 261-8
  • NAID: 10029363262
  • Peer Reviewed
• [Journal Article] アスベストの生態影響に関する研究について (2007)
  • Author(s): 大槻剛巳,前田恵,林宏明,董茂龍,三浦由恵,西村泰光
  • Journal Title: 安全医学(Journal of Medical Safety) 3(2) Pages: 92-103
• [Journal Article] 珪酸・アスベストの免疫系への影響 (2007)
  • Author(s): 大槻剛巳,前田恵,三浦由恵,兵藤文則,植木絢子,村上周子,林宏明,勝山博信,冨田正文,西村泰光
  • Journal Title: 日本職業災害医学会会誌 55(3) Pages: 113-120
  • Peer Reviewed
• [Journal Article] Keynote lecture in the 13th Japanese Society of Immunotoxicology (JSIT 2006)-Pathophysiological Development and Immunotoxicology:what we have found from the researches related to the silica and silicate such as asbestos- (2007)
  • Author(s): Takemi Otsuki
  • Journal Title: Environmental Health and Preventive Medicine 12 Pages: 153-164
  • Peer Reviewed
• [Journal Article] Immunological effects of silica and asbestos (2007)
  • Author(s): Takemi Otsuki
  • Journal Title: Cellular & Molecular Immunology 4 Pages: 261-268
  • Peer Reviewed
• [Journal Article] Long-lasting production of TGF-beta1 by alveolar macrophages exposed to low doses of asbestos without apoptosis (2007)
  • Author(s): Yasumitsu Nishimura
  • Journal Title: International Journal of Immunopathology and Pharmacology 20 Pages: 661-667
  • Peer Reviewed
• [Journal Article] Long-lasting production of TGF-beta1 by alveolar macrophages exposed to low doses of asbestos without apoptosis (2007)
  • Author(s): Yasumitsu Nishimura
  • Journal Title: International Journal of Immunopathology and Pharmacology 2 Pages: 661-667
  • Peer Reviewed
• [Journal Article] アスベストの生態影響に関する研究について (2007)
  • Author(s): 大槻剛巳
  • Journal Title: 安全医学 3 Pages: 92-93
• [Journal Article] Impairment in cytotoxicity and expression of NK-cell activating receptors on human NK cells following exposure to asbestos fibers
  • Author(s): Nishimura Y, Miura Y, Maeda M, KUmagai N, Murakami S, Hayashi H, Fukuoka K, Nakano T, Otsuki T
  • Journal Title: Int J Immunopathol Pharmacol (in press)
  • Peer Reviewed
• [Journal Article] Cytokine alteration and speculated immunological pathophysiology in silicosis and asbestos-related diseases
  • Author(s): Murakami S, Nishimura Y, Maeda M, Kumagai N, Hayashi H, Chen Y, Kusaka K, Kishimoto T, Otsuki t
  • Journal Title: Environ Health Prev Med (in press)
  • NAID: 10026958932
  • Peer Reviewed
• [Presentation] アスベスト曝露のNK細胞・CTL機能への抑制的影響、NKp46の悪性中皮腫予防分子指標の可能性 (2009)
  • Author(s): 西村泰光
  • Organizer: 第79回日本衛生学会総会,分子予防環境医学研究会シンポジウム「労働環境衛生への分子医学的アプローチ」
  • Place of Presentation: 北里大学白金キャンパス
  • Year and Date: 2009-03-30
• [Presentation] 石綿曝露によるNK細胞上NKp46発現抑制機構の解析,及び石綿関連疾患患者群間でのNKp46発現量の比較 (2009)
  • Author(s): 西村泰光,前田恵,熊谷直子,村上周子,林宏明,陳瑩,岸本卓巳,大槻剛巳
  • Organizer: 第79回日本衛生学会総会
  • Place of Presentation: 北里大学白金キャンパス
• [Presentation] Suppressive effect of asbestos-exposure on cytotoxicity of human NK cells, and the possibility of NKp46 as a marker to monitor immune status in people exposed to asbestos (2009)
  • Author(s): Nishimura Y, Maeda M, Kumagai N, Murakami S, Hayashi H, Kishimoto T, Fukuoka K, Nakano T, Otsuki T.
  • Organizer: 第2回日中石綿シンポジウム, New CEntury Zhijiang Resort Hangzhou
  • Place of Presentation: Hangzhou, China
• [Presentation] 悪性中皮腫予防分子指標としてのNK細胞上NKp46発現量低下の検討、および石綿曝露後NKp46発現抑制機構の解析 (2009)
  • Author(s): 西村泰光,前田恵,熊谷直子,村上周子,林宏明,陳瑩,岸本卓巳,大槻剛巳
  • Organizer: 第82回日本産業衛生学会
  • Place of Presentation: 福岡国際会議場
• [Presentation] 石綿曝露培養後の末梢血NK細胞におけるNKp46発現低下および石綿曝露NK細胞亜株YT-CB5におけるERKリン酸化低下 (2008)
  • Author(s): 西村泰光,前田恵,村上周子,熊谷直子,林宏明,三浦由恵,大槻剛巳
  • Organizer: 第78回日本衛生学会総会
  • Place of Presentation: 熊本市民会館
• [Presentation] 悪性中皮腫患者のNK細胞機能低下における加齢と石綿曝露の関連性の検討 (2008)
  • Author(s): 西村泰光,前田恵,熊谷直子,村上周子,林宏明,三浦由恵,岸本卓巳,大槻剛巳
  • Organizer: 日本基礎老化学会第31回大会
  • Place of Presentation: 長野県松本文化会館国際会議室
• [Presentation] 悪性中皮腫患者末梢血NK細胞と符合する石綿曝露培養後NK細胞上のNKp46発現低下 (2008)
  • Author(s): 西村泰光,前田恵,村上周子,林宏明,三浦由恵,大槻剛巳
  • Organizer: 第81回日本産業衛生学会
  • Place of Presentation: 札幌コンベンションセンター
• [Presentation] 悪性中皮腫患者NK細胞および石綿曝露後ヒトPBMC中NK細胞に共通するNKp46発現量の低下 (2008)
  • Author(s): 西村泰光,前田恵,熊谷直子,村上周子,林宏明,陳瑩,三浦由恵,岸本卓巳,大槻剛巳
  • Organizer: 第15回日本免疫毒性学会学術大会
  • Place of Presentation: タワーホール船堀
• [Presentation] 悪性中皮腫患者NK細胞におけるNKp46発現量低下と細胞傷害性低下の相関性 (2008)
  • Author(s): 西村泰光,前田恵,村上周子,熊谷直子,林宏明,三浦由恵,岸本卓巳,大槻剛巳
  • Organizer: 第49回日本肺癌学会総会
  • Place of Presentation: 西日本総合展示場/北九州国際会議場
• [Presentation] 悪性中皮腫患者末梢血中に観察されアスベスト曝露下で誘導されるNK細胞上NKp46発現低下/Decreased expression of NKp46 on NK cells in peripheral bloodof patients with malignant mesothelioma and in PBMCs culturedunder exposure to asbestos (2008)
  • Author(s): NISHIMURA Yasumitsu , MAEDA Megumi ,KUMAGAI Naoko , MURAKAMI Shuko ,HAYASHIHiroaki,OTSUKI Takemi
  • Organizer: 第38回日本免疫学会学術集会
  • Place of Presentation: 京都国際会館
• [Presentation] Impairment in cytotoxicity and expression of NK-cell activating receptors on human NK cells caused by exposure to asbestos fibers (2008)
  • Author(s): Yasumitsu Nishimura
  • Organizer: The 9th International Conference of the International Mesothelioma Interesting Group
  • Place of Presentation: Amsterdam
• [Presentation] Down-regulation of chemokine receptor CXCR3 in peripheral T lymphocytes from patients with asbestos-related disease (2008)
  • Author(s): Megumi Maeda
  • Organizer: The 9th International Conference of the International Mesothelioma Interesting Group
  • Place of Presentation: Amsterdam
• [Presentation] Keynote Speach (3). Achivement of Research Project "Comprehensive approach on asbestos-related diseases (sponsered by SCFPST)" and immunological effects of asbestos (2008)
  • Author(s): Takemi Otsuki
  • Organizer: AAI (Asian Asebestos Initiative) International Conference
  • Place of Presentation: Kitakyushu
• [Presentation] The functional impairment of NK cells due to the long-term exposure to asbestos (2007)
  • Author(s): Yasumitsu Nishimura
  • Organizer: 18th Japan-China-Korea Joint Conference on Occupational Health
  • Place of Presentation: Nagoya Congress Center
• [Presentation] Immunological Effects of Asbestos (2007)
  • Author(s): Megumi Maeda
  • Organizer: 18th Japan-China-Korea Joint Conference on Occupational Health
  • Place of Presentation: Nagoya Congress Center
• [Book] In Asbestos : Risks, Environment and Impact. Eds : Antonio Soto and Gael Salazar, ISBN 978-1-60692-053-4, c 2009 Nova Science Publishers, Inc (2009)
  • Author(s): Otsuki T , Maeda M, Miura Y, 林H, Murakami S, Kumagai N, Nishimura Y
  • Publisher: Immunological effects of Asbestos(in press)
• [Book] アスベストーシス.In炎症・再生医学事典.編:松島綱治,西脇徹 (2009)
  • Author(s): 林宏明,西村泰光,前田恵,大槻剛巳
  • Publisher: 朝倉書店