| Budget Amount *help |
¥4,810,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥810,000)
Fiscal Year 2010: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2009: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2008: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2007: ¥1,300,000 (Direct Cost: ¥1,300,000)
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| Research Abstract |
To detect the causal agents of otitis media associated with respiratory infection, we investigated 117 nasal swabs collected from children less than 2 years old who had been suspected to infect by RS virus. Using three multiplex RT nested PCR methods and two conventional nested PCR methods developed in this study, 91.5% of all samples were containing more than one viral agent. Of all, RS virus was detected in 61 (52.1%), Rhino or Enterovirus was detected in 46 (39.3%), Adenovirus was detected in 44 (37.6%), human Bocavirus was detected in 21 (19.7%), Parainfluenza virus was detected in 9 (7.7%), human Metapneumovirus and Coronavirus was detected in one specimen (1.7%). Co-infection was detected in 57 cases (48.7%) and among them, 38 specimens (66.6%) contained two, 16 specimens (28.27%) contained three and 3 specimens (5.2%) contained four agents. Co-infection with RS virus and Adenovirus was most commonly detected in this study (21%).
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