| Project/Area Number |
19790451
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Legal medicine
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| Research Institution | National Research Institute of Police Science |
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Principal Investigator |
TSUJIKAWA Kenji National Research Institute of Police Science, 法科学第三部化学第一研究室, 研究員 (50356193)
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| Project Period (FY) |
2007 – 2009
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| Project Status |
Completed (Fiscal Year 2009)
|
| Budget Amount *help |
¥2,430,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2009: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2008: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2007: ¥1,000,000 (Direct Cost: ¥1,000,000)
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| Keywords | 法医鑑定学 / 薬毒物スクリーニング |
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| Research Abstract |
The aim of this study is to develop a screening method for 38 central nervous system drugs such as hypnotics, antidepressants, antipsychotics. The drug standards were spiked to methanol homogenate of porcine liver, then clean-up was performed as following order : i) fat precipitation by cooling, ii) hexane-acetonitrile partition, iii) liquid-liquid extraction under basic condition. The sample prepared was determined by liquid chromatography tandem mass spectrometry. The recovery and repeatability (CV%) of the targeted drugs except for 7-aminobenzodiazepines (3 drugs) were more than 40% and within 15%, respectively. The method would be useful for drug screening in solid tissues.
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