Role of nitric oxide synthase isozyme in brain CRF-induced sympathetic activation

• Project/Area Number: 19790457
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: General internal medicine (including Psychosomatic medicine)
• Research Institution: Kochi University
• Principal Investigator: USUI Daisuke Kochi University, 教育研究部医療学系, 助教 (70351913)
• Project Period (FY): 2007 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥3,090,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥390,000); Fiscal Year 2008: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2007: ¥1,400,000 (Direct Cost: ¥1,400,000)
• Keywords: CRF / 一酸化窒素合成酵素 / 視床下部室傍核 / Fos / 交感神経-副腎髄質系 / c-Fos

Research Abstract

副腎皮質刺激ホルモン放出因子(CRF)による交感神経系賦活の調節因子として一酸化窒素合成酵素(NOS)に着目し、CRFを脳室内投与したラットの視床下部室傍核におけるNOS発現の解析を行った。CRF投与によりiNOS発現は有意に増加し、一方nNOS発現の変化はみられなかった。また、iNOSおよびnNOS陽性細胞は各々、CRFにより活性化した。
以上より、CRFによる交感神経系賦活には主としてiNOSが関与している可能性が示された。

Research Products

• [Journal Article] Selective activation of the sympathetic ganglia by centrally administered corticotropin-releasing factor in rats (2009)
  • Author(s): Usui D, Yamaguchi-Shima N, Okada S, Shimizu T, Wakiguchi H, Yokotani K
  • Journal Title: Auton. Neurosci 146 Pages: 111-114
  • Peer Reviewed
• [Journal Article] Selective activation of the sympathetic ganglia by centrally administered corticotropin-releasing factor in rats (2009)
  • Author(s): Usui D, et.al.
  • Journal Title: Autonomic Neuroscience 146 Pages: 111-114
• [Journal Article] Central bombesin activates adrenal adrenaline-and noradrenaline-containing cells via brain thromboxane A_2 in rats (2009)
  • Author(s): Usui D, et.al.
  • Journal Title: Autonomic Neuroscience (印刷中)
  • Peer Reviewed
• [Journal Article] Nitric oxide synthase isozymes in spinally projecting PVN neurons are involved in CRF-induced svmpathetic activation (2009)
  • Author(s): Yamaguchi N, et.al.
  • Journal Title: Autonomic Neuroscience (印刷中)
  • Peer Reviewed
• [Journal Article] Adrenal adrenaline- and noradrenalinecontaining cells and celiac sympathetic ganglia are differentially controlled by centrally administered corticotropin-releasing factor and arginine-vasopressin in rats (2007)
  • Author(s): Yamaguchi-Shima N, Okada S, Shimizu T, Usui D, Nakamura K, Lu L, Yokotani K
  • Journal Title: Eur. J.Pharmacol 564 Pages: 94-102
  • Peer Reviewed
• [Journal Article] Adrenal adrenaline-and noradrenaline-containing cells and celiac sympathetic ganglia are differentially controlled by centrally administered corticotropin-releasing factor and arginine-vasopressin in rats. (2007)
  • Author(s): Yamaguchi-Shima N, Okada S, Shimizu T, Usui D, Nakamura K, Lu L, Yokotani K
  • Journal Title: European Journal of Pharmacology 564 Pages: 94-102
  • NAID: 120001073504
  • Peer Reviewed
• [Journal Article] Nitric oxide synthase isozymes in spinally projecting PVN neurons are involved in CRF-induced sympathetic activation
  • Author(s): Yamaguchi N, Okada S, Usui D, Yokotani K
  • Journal Title: Auton. Neurosci (印刷中)
  • Peer Reviewed
• [Journal Article] Central bombesin activates adrenal adrenaline-and noradrenalinecontaining cells via brain thromboxane A2 in rats
  • Author(s): Usui D, Yamaguchi-Shima N, Okada S, Shimizu T, Wakiguchi H, Yokotani K
  • Journal Title: Auton. Neurosci (印刷中)
  • Peer Reviewed
• [Presentation] 脳室内投与CRFによる交感神経-副腎髄質系における視床下部室傍核での一酸化窒素合成酵素の役割 (2007)
  • Author(s): 臼井大介, 他
  • Organizer: 第30回日本神経科学大会
  • Place of Presentation: 横浜市
  • Year and Date: 2007-09-11
• [Presentation] 脳室内投与CRF による交感神経-副腎髄質系における視床下部室傍核での一酸化窒素合成酵素の役割 (2007)
  • Author(s): 臼井大介他
  • Organizer: 第30 回日本神経科学大会
  • Place of Presentation: 横浜市