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Investigation of cancer stem cell system in hepatocellular carcinoma

Research Project

Project/Area Number 19790468
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeSingle-year Grants
Research Field Gastroenterology
Research InstitutionChiba University

Principal Investigator

CHIBA Tetsuhiro  Chiba University, 大学院医学研究院, 助教 (00381583)

Co-Investigator(Renkei-kenkyūsha) IWAMA Atsushi  千葉大学, 大学院医学研究院, 教授 (70244126)
MIYAGI Satoru  千葉大学, 大学院医学研究院, 助教 (20400997)
YOKOSUKA Osamu  千葉大学, 大学院医学研究院, 教授 (90182691)
Project Period (FY) 2007 – 2008
Project Status Completed (Fiscal Year 2008)
Budget Amount *help
¥3,690,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2008: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2007: ¥2,000,000 (Direct Cost: ¥2,000,000)
Keywords癌幹細胞 / 肝臓癌 / 自己複製能 / Bmi1 / CD133, CD90 / Bmil / CD133 / CD90 / フローサイトメトリー / 自己複製 / SP細胞
Research Abstract

肝癌における癌幹細胞システムの解明を目的とし、肝癌臨床検体からの癌幹細胞分離と、ヒト肝癌培養細胞およびマウス肝幹細胞を用いた癌幹細胞システムの検証を行った。肝癌臨床検体からは、CD133 陽性細胞、CD90 陽性細胞を検出できた。また、肝癌培養細胞から分離したsidepopulation 細胞が癌幹細胞として機能し、その腫瘍形成能がBmi1 によって維持されていることや、Bmi1 を強制発現した肝幹細胞において、Bmi1 の主要な標的分子であるInk4a/Arf 遺伝子以外に、より緊密に癌化機転に寄与する分子群の存在を見出した。

Report

(3 results)
  • 2008 Annual Research Report   Final Research Report ( PDF )
  • 2007 Annual Research Report
  • Research Products

    (14 results)

All 2009 2008 2007 Other

All Journal Article (7 results) (of which Peer Reviewed: 7 results) Presentation (7 results)

  • [Journal Article] Distinct expression of polycomb group proteins EZH2 and BMI1 in hepatocellular carcinoma2009

    • Author(s)
      Yonemitsu Y.
    • Journal Title

      Human Pathology (in press)

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Stem cells and cancer in the liver.2008

    • Author(s)
      Taniguchi H, Chiba T.
    • Journal Title

      Dis Markers 24

      Pages: 223-229

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] The polycomb gene product BMI1 contributes to the maintenance of tumor-initiating side population cells in hepatocellular carcinoma.2008

    • Author(s)
      Chiba T, Miyagi S, Saraya A, Ryutaro Aoki R, Seki A, Morita Y, Yonemitsu Y, Yokosuka O, Taniguchi H, Nakauchi H,Iwama A.
    • Journal Title

      Cancer Res 68

      Pages: 7742-7749

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] The polycomb gene product BMI1 contributes to the maintenance of tumorinitiating side population cells in hepatocellular carcinoma2008

    • Author(s)
      Chiba T.
    • Journal Title

      Cancer Research 68

      Pages: 7742-7749

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Enhanced self-renewal capability in hepatic stem/progenitor cells drives cancer initiation.2007

    • Author(s)
      Chiba T, Zheng YW, Kita K, Yokosuka O, Saisho H, Onodera M, Miyoshi M, Nakano M, Nakauchi H, Iwama A, Taniguchi H.
    • Journal Title

      Gastroenterology 133

      Pages: 937-950

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Enhanced self-renewal capability in hepatic stem/progenitor cells drives cancer initiation.2007

    • Author(s)
      Chiba T, et. al.
    • Journal Title

      Gastroenterology 133

      Pages: 937-950

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Distinct expression of polycomb group proteins EZH2 and BMI1 in hepatocellular carcinoma.

    • Author(s)
      Yonemitsu Y, Imazeki F, Chiba T, Fukai K, Nagai Y, Miyagi S, Arai M, Aoki R, Miyazaki M, Nakatani Y, Iwama A, Yokosuka O.
    • Journal Title

      Hum Pathol (in press.)

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Presentation] Molecular machinery in the establishment and maintenance of liver cancer stem cell system.2008

    • Author(s)
      千葉哲博、宮城聡、横須賀收、岩間厚志
    • Organizer
      第67回日本癌学会総会
    • Place of Presentation
      愛知県
    • Year and Date
      2008-10-29
    • Related Report
      2008 Final Research Report
  • [Presentation] Molecular machinery in the establishment and maintenance of liver cancer stem cell system2008

    • Author(s)
      千葉哲博
    • Organizer
      第67回日本癌学会総会
    • Place of Presentation
      名古屋国際会議場
    • Year and Date
      2008-10-29
    • Related Report
      2008 Annual Research Report
  • [Presentation] 肝臓における癌幹細胞システムの成立・維持メカニズム2008

    • Author(s)
      千葉哲博、横須賀收、岩間厚志
    • Organizer
      第16回日本消化器病学会関連週間
    • Place of Presentation
      東京都
    • Year and Date
      2008-10-01
    • Related Report
      2008 Final Research Report
  • [Presentation] 肝臓における癌幹細胞システムの成立・維持メカニズム2008

    • Author(s)
      千葉哲博
    • Organizer
      第16回日本消化器病学会関連週間
    • Place of Presentation
      グランドプリンスホテル高輪
    • Year and Date
      2008-10-01
    • Related Report
      2008 Annual Research Report
  • [Presentation] The polycomb gene product BMI1 is required for the tumor-initiating capacity of hepatocellular carcinoma cells.2007

    • Author(s)
      千葉哲博、宮城聡、横須賀收、岩間厚志
    • Organizer
      第66回日本癌学会総会
    • Place of Presentation
      神奈川県
    • Year and Date
      2007-10-03
    • Related Report
      2008 Final Research Report
  • [Presentation] ポリコーム遺伝子産物Bmi1は肝癌細胞の腫瘍形成能維持に必須である2007

    • Author(s)
      千葉哲博
    • Organizer
      第66回日本癌学会学術総会
    • Place of Presentation
      パシフィコ横浜
    • Year and Date
      2007-10-03
    • Related Report
      2007 Annual Research Report
  • [Presentation] Investigation of cancer stem cell system in hepatocellular carcinoma.2007

    • Author(s)
      Chiba T, Miyagi S, Yokosuka O, Taniguchi H, Nakauchi H, Iwama A.
    • Organizer
      17th APASL conference
    • Place of Presentation
      Kyoto, Japan
    • Year and Date
      2007-03-29
    • Related Report
      2008 Final Research Report

URL: 

Published: 2007-04-01   Modified: 2016-04-21  

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