| Budget Amount *help |
¥3,800,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥600,000)
Fiscal Year 2009: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2008: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2007: ¥1,200,000 (Direct Cost: ¥1,200,000)
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| Research Abstract |
The investigation to determine an appropriate staining regimen for endocytoscopy revealed that the optimum conditions for endocytoscopic observation were obtained after staining with 1% methylene blue in the esophagus and with 0.25% toluidine blue in the stomach and the colon, after 60 seconds of exposure to the dye. When the conditions were applied to the living beagles, reliable observations in the stomach were not obtained in the stomach due to thick mucous layer on the surface epithelia. Focusing on the esophageal observations, in-vivo endocytoscopic images were quite similar with the horizontal histology of fixed specimens with H&E staining. We obtained a novel finding that computer-assisted analysis of nuclear density between malignant and normal epithelia can lead to automatic diagnosis by using the endocytoscopy.
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