Basic investigation of Hepatitis B antiviral therapy that contemplates custom-made treatment
| Project/Area Number |
19790471
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Gastroenterology
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| Research Institution | The University of Tokyo |
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Principal Investigator |
GOTO Tadashi The University of Tokyo, 医学部附属病院, 助教 (40444088)
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| Project Period (FY) |
2007 – 2008
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| Project Status |
Completed (Fiscal Year 2008)
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| Budget Amount *help |
¥3,690,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2008: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2007: ¥2,000,000 (Direct Cost: ¥2,000,000)
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| Keywords | 肝臓病学 / B型肝炎ウイルス / 抗ウイルス療法 / 薬剤耐性 / TaqMan PCR |
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| Research Abstract |
ラミブジン耐性株のquasispeciesを検討した。TaqMan-MGB probe assayにより,YVDD 変異 に関しては2.0 logコピー中10% (=10 logコピー)の存在,YIDD変異に関しては2.7 logコピー中に10% (=50 log コピー)の存在の検出が可能となった。またHBV DNAが2.6 logコピー(最近までの検出限界量)未満の臨床検体でも変異株を検出でき,感度は従来のDirect Sequencing 法,INNO-LiPA法を上回った。
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Report
(3 results)
Research Products
(14 results)
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[Journal Article] Rapid detection of the hepatitis B virus YMDD mutant using TaqMan-minor groove binder probes.2008
Author(s)
Hua R, Tanaka Y, Fukai K, Tada M, Seto M, Asaoka Y, Ohta M, Goto T, Kanai F, Kato N, Yoshida H, Kawabe T, Yokosuka O, Omata M
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Journal Title
Clinica Chemica Acta 395
Pages: 151-154
Related Report
Peer Reviewed
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