| Budget Amount *help |
¥3,770,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥570,000)
Fiscal Year 2009: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2008: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2007: ¥1,300,000 (Direct Cost: ¥1,300,000)
|
|---|
| Research Abstract |
We introduced human connective tissue growth factor (CTGF) cDNA into the E1 region of cosmid vectors in order to create recombinant adenovirus CTGF (adeno-CTGF). Control adenovirus vectors into which adeno-CTGF and Escherichia coli beta-galactosidase genes were introduced were intravenously injected into 7-week-old male Wister rats, and their hepatic tissues were harvested 3 and 7 days later. CTGF was expressed in the liver 3 days after the administration, but its expression was not observed 7 days after the administration. A comprehensive analysis of gene expression by GeneChip using hepatic tissue showed that gene expression in fibrosis had been increased both 3 and 7 days later.
|
