Analysis of idiopathic portal hypertension

• Project/Area Number: 19790492
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Gastroenterology
• Research Institution: Osaka City University
• Principal Investigator: KAWAMURA Etsushi Osaka City University, 大学院・医学研究科, 後期臨床研究医 (60419710)
• Project Period (FY): 2007 – 2009
• Project Status: Completed (Fiscal Year 2009)
• Budget Amount: ¥3,770,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥570,000); Fiscal Year 2009: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000); Fiscal Year 2008: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2007: ¥1,300,000 (Direct Cost: ¥1,300,000)
• Keywords: CTGF / 特発性門脈圧亢進症 / 動物モデル / 組み換えアデノウイルス / 重量分析 / プロテオーム解析 / ネットワーク解析 / LC-Ms / Msシステム / アデノウイルスベクター / 遺伝子導入 / TGF-β / thioacetamide / クラスター解析

Research Abstract

We introduced human connective tissue growth factor (CTGF) cDNA into the E1 region of cosmid vectors in order to create recombinant adenovirus CTGF (adeno-CTGF). Control adenovirus vectors into which adeno-CTGF and Escherichia coli beta-galactosidase genes were introduced were intravenously injected into 7-week-old male Wister rats, and their hepatic tissues were harvested 3 and 7 days later. CTGF was expressed in the liver 3 days after the administration, but its expression was not observed 7 days after the administration. A comprehensive analysis of gene expression by GeneChip using hepatic tissue showed that gene expression in fibrosis had been increased both 3 and 7 days later.

Research Products

• [Journal Article] 特発性門脈圧亢進症の病因・病態に関する解析と動物モデルの作製 (2009)
  • Author(s): 塩見進, 川村悦史, 森川浩安, 榎本大, 田守昭博, 河田則文
  • Journal Title: 厚生労働省特定疾患門脈血行異常症調査研究班平成二十年度研究報告書 Pages: 17-20
• [Journal Article] A randomized pilot trial of oral branched-chain amino acids in early cirrhosis: validation using prognostic markers for pre-liver transplant status (2009)
  • Author(s): Kawamura E, Habu D, Morikawa H, Enomoto M, Kawabe J, Tamori A, Sakaguchi H, Saeki S, Kawada N, Shiomi S.
  • Journal Title: Liver Transpl 15 Pages: 790-797
  • Peer Reviewed
• [Journal Article] 特発性門脈圧亢進症の病因・病態に関する解析と動物モデルの作製 (2009)
  • Author(s): 塩見進、川村悦史, 他
  • Journal Title: 厚生労働省特定疾患門脈血行異常症調査研究班平成二十年度研究報告書 Pages: 17-20
• [Journal Article] 質量分析を用いたIPH肝組織特異タンパク質の解析 (2009)
  • Author(s): 塩見進、川村悦史, 他
  • Journal Title: 厚生労働省特定疾患門脈血行異常症調査研究班平成二十年度研究報告書 Pages: 74-76
• [Journal Article] A randomized pilot trial of oral branched-chain amino acids in early cirrhosis : validation using prognostic markers for pre-liver transplant status (2009)
  • Author(s): Kawamura E, Shiomi S, et al.
  • Journal Title: Liver Transpl 15 Pages: 790-797
  • Peer Reviewed
• [Journal Article] CTGF組み換えアデノウイルス導入ラットによる肝組織内遺伝子発現の動態とクラスター解析 (2008)
  • Author(s): 塩見進, 川村悦史, 森川浩安, 田守昭博, 羽生大記, 河田則文
  • Journal Title: 厚生労働省特定疾患門脈血行異常症調査研究班平成十九年度研究報告書 Pages: 25-28
• [Journal Article] Clinical role of FDG-PET for HCC: relationship of glucose metabolic indicator to Japan Integrated Staging (JIS) score. (2008)
  • Author(s): Kawamura E, Habu D, Ohfuji S, Fukushima W, Enomoto M, Torii K, Kawabe J, Kondo K, Tamoi A, Kawada N, Shiomi S.
  • Journal Title: Hepato-gastroenterology 55 Pages: 582-586
  • Peer Reviewed
• [Journal Article] CTGF組み換えアデノウイルス導入ラットによる肝組織内遺伝子発現の動態とクラスター解析 (2008)
  • Author(s): 塩見進、川村悦史、他
  • Journal Title: 厚生労働省特定疾患門脈血行異常症調査研究班平成十九年度研究報告書 Pages: 25-28
• [Journal Article] Clinical role of FDG-PET for HCC : relationship of glucose metabolic indicator to Japan Integrated Staging (JIS) score (2008)
  • Author(s): Kawamura E, Habu D, et.al.
  • Journal Title: Hepatogastroenterology 55 Pages: 582-586
  • Peer Reviewed
• [Journal Article] CTGF組み換えアデノウイルス感染ラットにおける肝組織における網羅的遺伝子発現の解析 (2007)
  • Author(s): 塩見進, 川村悦史, 森川浩安, 田守昭博, 羽生大記, 河田則文
  • Journal Title: 厚生労働省特定疾患門脈血行異常症調査研究班平成十八年度研究報告書 Pages: 19-24
• [Journal Article] CTGF組み換えアデノウイルス感染ラットにおける肝組織における網羅的遺伝子発現の解析 (2007)
  • Author(s): 塩見 進、 川村 悦史、 他
  • Journal Title: 厚生労働省特定疾患門脈血行異常症調査研究班平成十八年度研究報告書 Pages: 19-24
• [Presentation] Induction of fibrotic gene expression by adenoviral gene transfer of connective tissue growth factor in the rat liver (2008)
  • Author(s): Morikawa H, Shiomi S, Kawamura E, Kobayashi S, Iwai S, Enomoto M, Tamori A, Sakaguchi H, Kawada N
  • Organizer: Seventh JSH Single Topic Conference
  • Place of Presentation: Fukuoka (Japan)
  • Year and Date: 2008-11-22
• [Presentation] Induction of fibrotic gene expression by adenoviral gene transfer of connective tissue growth factor in the rat liver (2008)
  • Author(s): Morikawa H, Kawamura E, et.al.
  • Organizer: Seventh JSH Single Topic Conference
  • Place of Presentation: Fukuoka
  • Year and Date: 2008-11-22
• [Presentation] A randomized pilot trial of oral branched-chain amino acids in early liver cirrhosis. (2008)
  • Author(s): Kawamura E, Iwai S, Tamori A, Enomoto M, Takeda T, Habu D, Sakaguchi H, Kwada N, Shiomi S, Kawada N
  • Organizer: 43rd Annual Meeting of the European Association for the Study of the Liver
  • Place of Presentation: Milan (Italy)
  • Year and Date: 2008-04-23
• [Presentation] A randomized pilot trial of oral branched-chain amino acids in early liver cirrhosis (2008)
  • Author(s): Kawamura E, Habu D, et.al.
  • Organizer: 43rd Annual Meeting of the European Association for the Study of the Liver
  • Place of Presentation: Milan, Italy
  • Year and Date: 2008-04-23
• [Presentation] Induction of fibrotic gene expression by adenoviral gene transfer of connective tissue growth factor in the rat liver (2007)
  • Author(s): Morikawa H, Tamori A, Kawamura E, Enomoto M, Takeda T, Habu D, Sakaguchi H, Nishiguchi S, Shiomi S, Kawada N
  • Organizer: United European Gastroenterology Week
  • Place of Presentation: Paris (France)
  • Year and Date: 2007-10-28
• [Presentation] Induction of fibrotic gene expression by adenoviral gene transfer of connective tissue growth factor in the rat liver (2007)
  • Author(s): Morikawa H, kawamura E, et. al.
  • Organizer: United European Gastroenterology Week
  • Place of Presentation: Paris
  • Year and Date: 2007-10-28