Esophageal mucosal defense mechanisms and a new pathophysiological classification by intercellular junctional complex

• Project/Area Number: 19790501
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Gastroenterology
• Research Institution: Hyogo College of Medicine
• Principal Investigator: OSHIMA Tadayuki Hyogo College of Medicine, 医学部, 講師 (00381814)
• Project Period (FY): 2007 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥3,630,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥330,000); Fiscal Year 2008: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2007: ¥2,200,000 (Direct Cost: ¥2,200,000)
• Keywords: 逆流性食道炎 / タイト結合 / クローディン

Research Abstract

食道粘膜防御機構を解析する上で,種々の実験的操作を行うには不可欠である食道粘膜上皮様重層扁平上皮初代培養細胞系を世界で初めて確立した.また粘膜透過性上昇には,酸以外に胆汁酸が重要な役割をしていることを見出した.
一方ヒト食道生検組織では,非びらん性食道炎患者の食道粘膜上皮基底層の細胞間間隙の開大が認められ,この開大とタイト結合蛋白の発現との相関を明らかとすべく継続して解析を進めている.

Research Products

• [Journal Article] Experimental oesophagitis in the rat is associated with decreased voluntary movement (2009)
  • Author(s): Miwa H, Oshima T, Sakurai J, Tomita T, Matsumoto T, Iizuka S, Koseki J.
  • Journal Title: Neurogastroenterol Motil 21 Pages: 296-303
  • Peer Reviewed
• [Journal Article] 非びらん性胃食道逆流症(NERD)の病態と治療 (2009)
  • Author(s): 大島忠之,三輪洋人
  • Journal Title: 日本消化器病学会雑誌 106巻3号 Pages: 327-334
  • NAID: 130000444570
• [Journal Article] Gastro-esophageal reflux disease: the recent trend in Japan (2008)
  • Author(s): Miwa H, Oshima T, Tomita T, Kim Y, Hori K, Matsumoto T
  • Journal Title: Clin J Gastroenterol 1 Pages: 133-8
  • Peer Reviewed
• [Journal Article] Changes in the expression of claudins in active ulcerative colitis (2008)
  • Author(s): Oshima T, Miwa H, Joh T.
  • Journal Title: J Gastroenterol Hepatol 23
  • Peer Reviewed
• [Journal Article] Oxidative stress induces gastric epithelial permeability through claudin-3 (2008)
  • Author(s): Hashimoto K, Oshima T, Tomita T, Kim Y, Matsumoto T, Joh T, Miwa H
  • Journal Title: Biochem Biophys Res Commun 376 Pages: 154-7
  • Peer Reviewed
• [Journal Article] Aspirin induces gastric epithelial barrier dysfunction by activating p38 MAPK via claudin-7 (2008)
  • Author(s): Oshima T, Miwa H, Joh T
  • Journal Title: Am J Physiol Cell Physiol 295
  • Peer Reviewed
• [Journal Article] Interobserver agreement in endoscopic evaluation of reflux esophagitis using a modified Los Angeles classification incorporating grades N and M: a validation study in a cohort of Japanese endoscopists (2008)
  • Author(s): Miwa H, Yokoyama T, Hori K, Sakagami T, Oshima T, Tomita T, Fujiwara Y, Saita H, Itou T, Ogawa H, Nakamura Y, Kishi K, Murayama Y, Hayashi E, Kobayashi K, Tano N, Matsushita K, Kawamoto H, Sawada Y, Ohkawa A, Arai E, Nagao K, Hamamoto N, Sugiyasu Y, Sugimoto K, Hara H, Tanimura M, Honda Y, Isozaki K, Noda S, Kubota S, Himeno S
  • Journal Title: Dis Esophagus 21 Pages: 355-63
  • Peer Reviewed
• [Journal Article] Does magnifying endoscopy improve the diagnosis of erosive esophagitis? (2008)
  • Author(s): Amano Y, Yamashita H, Koshino K, Ohshima T, Miwa H, Iwakiri R, Fujimoto K, Manabe N, Haruma K, Kinoshita Y.
  • Journal Title: J Gastroenterol Hepatol 23 Pages: 1063-8
  • Peer Reviewed
• [Journal Article] Wip1 protects hydrogen peroxide-induced colonic epithelial barrier dysfunction (2007)
  • Author(s): Oshima T, Sasaki M, Kataoka H, Miwa H, Takeuchi T, Joh T
  • Journal Title: Cel lMol Life Sci 64 Pages: 3139-47
  • Peer Reviewed
• [Journal Article] 【生活習慣病と消化器疾患】生活習慣からみた消化器疾患胃食道逆流症と生活習慣 (2007)
  • Author(s): 大島忠之, 三輪洋人
  • Journal Title: 綜合臨床 56巻11号 Pages: 3029-3032
• [Journal Article] 【NERDの病態と治療】内臓知覚からみたNERDの病態 (2007)
  • Author(s): 大島忠之, 三輪洋人
  • Journal Title: G.I.Research 15巻3号 Pages: 196-201
• [Journal Article] 【胃食道逆流症(GERD)非びらん性逆流症(NERD)を含めて】GERDの病因論 (2007)
  • Author(s): 大島忠之, 三輪洋人
  • Journal Title: 日本臨床 65巻5号 Pages: 797-801
• [Presentation] Proton pump inhibitor treatment ameliorates dilated intercellular space, decrease and dislocalization of esophageal mucosal tight junction proteins in a rat chronic acid reflux esophagitis model (2008)
  • Author(s): Oshima T, Koseki J, Iizuka S, Tomita T, Matsumoto T, Sakurai J, Miwa H.
  • Organizer: Digestive Disease Week 2008
  • Place of Presentation: San Diego, California, USA
  • Year and Date: 2008-05-20
• [Presentation] Aspirin induced gastric epithelial barrier dysfunction and tight junction (2008)
  • Author(s): Oshima T, Joh T, Miwa H.
  • Organizer: The 1st International Gastrointestinal Consensus Symposium (IGICS)
  • Place of Presentation: Osaka, Japan
  • Year and Date: 2008-02-08
• [Presentation] Recent trend of GERD in Japan (2007)
  • Author(s): Miwa H, Hori K, Oshima T, Yamamoto N, Matsumoto T
  • Organizer: APDW 2007
  • Place of Presentation: Kobe, Japan
  • Year and Date: 2007-10-18
• [Presentation] Decreased rat voluntary movement as a measure of chronic visceral pain -a study using the rat chronic acid reflux esophagitis model (2007)
  • Author(s): Miwa H, Oshima T, Tomita T, Sakurai J, Matsumoto T, Koseki J
  • Organizer: Digestive Disease Week 2007
  • Place of Presentation: Washington DC, USA
  • Year and Date: 2007-05-23
• [Presentation] Aspirin induces gastric epithelial barrier dysfunction by activating p38 MAP kinase via claudin-7 (2007)
  • Author(s): Oshima T, Joh T, Kataoka H, Sasaki M, Matsumoto T, Miwa H
  • Organizer: Digestive Disease Week2007
  • Place of Presentation: Washington DC, USA
  • Year and Date: 2007-05-21