Development of the hepatitis virus infection model using 3D cultured hepatocyte

• Project/Area Number: 19790503
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Gastroenterology
• Research Institution: National Institute of Infectious Diseases
• Principal Investigator: MURAKAMI Kyoko National Institute of Infectious Diseases, ウイルス第二部, 主任研究官 (30399456)
• Project Period (FY): 2007 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥3,720,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥420,000); Fiscal Year 2008: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2007: ¥1,900,000 (Direct Cost: ¥1,900,000)
• Keywords: HBV / HEV / 三次元培養

Research Abstract

三次元培養した肝細胞に対するB型肝炎ウイルスの感染および複製の可否について検討した。その結果、簡易的な三次元培養系である温度感受性ゲルではB型肝炎ウイルスは感染できない可能性、人工肝臓で培養した三次元培養肝細胞にはB型肝炎ウイルスの感染が成立する可能性が示唆された。今後はこの結果についての再現性について確認するとともに、薬剤耐性ウイルス出現の評価へ応用可能であるか等を検討する必要があると考える。

Research Products

• [Journal Article] Cellular vimentin content regulates the protein level of hepatitis C virus core protein and the hepatitis C virus production in cultured cells. (2009)
  • Author(s): Nitahara-KasaharaY, Fukasawa M, Shinkai-Ouchi F, S, Suzuki T, Murakami K, Wakita T, Hanada K, Miyamura T, Nishijima M.
  • Journal Title: Virology 383(2) Pages: 319-327
  • Peer Reviewed
• [Journal Article] Replication of chicken anemia virus (CAV) requires apoptin and is complemented by VP3 of human torque teno virus (TTV). (2009)
  • Author(s): Prasetyo AA, Kamahora T, Kuroishi A, Murakami K, Hino S.
  • Journal Title: Virology (Epub ahead of print)
  • Peer Reviewed
• [Journal Article] Identification of Annexin A1 as a novel substrate for 1 E6AP-mediated ubiquitylation (2009)
  • Author(s): etsu Shimoji, Kyoko Murakami, Yuichi Sugiyama, Mami Matsuda, Sachiko Inubushi, Junichi Nasu, Masayuki Shirakura, Tetsuro Suzuki, Takaji Wakita, Tatsuya Kishino, Hak Hotta, Tatsuo Miyamura, Ikuo Shoji
  • Journal Title: Journal of Cellular Biochemistry (Epub ahead of print)
  • Peer Reviewed
• [Journal Article] Cellular vimentin content regulates the protein level of hepatitis C virus core protein and the hepatitis C virus production in cultured cells. (2009)
  • Author(s): Nitahara-Kasahara Y, Fukasawa M, Shinkai-Ouchi F, Sato S, Suzuki T, Murakami K. Wakita T, Hanada K, Miyamura T, Nishijima M.
  • Journal Title: Virology 383(2) Pages: 319-327
  • Peer Reviewed
• [Journal Article] Identification of Annexin Al as a novel substrate for 1 E6AP-mediated ubiquitylation (2009)
  • Author(s): Tetsu Shimoji, Kyoko Murakami, Yuichi Sugiyama, Mami Matsuda, Sachiko Inubushi, Junichi Nasu, Masayuki Shirakura, Tetsuro Suzuki, Takaji Wakita, Tatsuya Kishino, Hak Hotta, Tatsuo Miyamura, and Ikuo Shoji
  • Journal Title: Journal of Cellular Biochemistry (Epub ahead of print)
  • Peer Reviewed
• [Journal Article] Virological characterization of the HCV JFH-1 strain in lymphocytic cell lines (2008)
  • Author(s): Kyoko Murakami, Toshiro Kimura, Motonao Osaki, Koji Ishii, Tatsuo Miyamura, Tetsuro Suzuki, Takaji Wakita, Ikuo Shoji
  • Journal Title: J Gen Virol. 89 Pages: 1587-1592
  • Peer Reviewed
• [Journal Article] Trans- encapsidation of hepatitis C virus subgenomic replicon RNA with viral structure proteins (2008)
  • Author(s): Koji Ishii, Kyoko Murakami, Su Su Hmwe, Zhang Bin, Jin Li, Masayuki Shirakura, Kenichi Morikawa, Ryosuke Suzuki, Tatsuo Miyamura, Takaji Wakita, Tetsuro Suzuki
  • Journal Title: BBRC 371(3) Pages: 446-50
  • Peer Reviewed
• [Journal Article] Interaction of hepatitis C virus nonstructural protein 5A with core protein is critical for the production of infectious virus particles. (2008)
  • Author(s): Masaki T, Suzuki R, Murakami K, Aizaki H, Ishii K, Murayama A, Date T, Matsuura Y, Miyamura T, Wakita T, Suzuki T.
  • Journal Title: J Virol. 82(16) Pages: 7964-76
  • Peer Reviewed
• [Journal Article] Virological characterization of the HCV JFH-1 strain in lymphocytic cell lines; (2008)
  • Author(s): Kyoko Murakami, Toshiro Kimura, Motonao Osaki, Koji Ishii, Tatsuo Miyamura, Tetsuro Suzuki, Takaji Wakita and Ikuo Shoji
  • Journal Title: J Gen Virol. 89 Pages: 1587-1592
  • Peer Reviewed
• [Journal Article] Trans-encapsidation of hepatitis C virus subgenomic replicon RNA with viral structure proteins (2008)
  • Author(s): Koji Ishii, Kyoko Murakami, Su Su Hmwe, Zhang Bin, Jin Li, Masayuki Shirakura, Kenichi Morikawa, Ryosuke Suzuki, Tatsuo Miyamura, Takaji Wakita, Tetsuro Suzuki
  • Journal Title: BBRC 371(3) Pages: 446-450
  • Peer Reviewed
• [Journal Article] Replication of chicken anemia virus (CAV) requires apoptin and is complemented by VP3 of human torque teno virus (TTV). (2008)
  • Author(s): Prasetyo AA, Kamahora T, Kuroishi A, Murakami K. Hino S.
  • Journal Title: Virology (Epub ahead of print)
  • Peer Reviewed
• [Journal Article] Dynamic behavior of hepatitis C virus quasispecies in a Iong-term culture of the three-dimensional radial-flow bioreaator system (2008)
  • Author(s): Kyoko Murakami. Yasushi Inoue, Su-Su Hmwe, Kazzuhiko Omata, Tomokatsu Hongo, Koji Ishii, Sayaka Yoshizaki, Hideki Aizaki, Tomokazu Matsura, Shoji, Tatsuo Miyamura, and Tetswo Suzuki
  • Journal Title: J.Virol.Methods 148(1-2) Pages: 174-181
  • Peer Reviewed
• [Journal Article] Molecular biology of hepatitis C virus (2007)
  • Author(s): Suzuki T, Aizaki H, Murakami K, Shoji I, Wakita T;
  • Journal Title: J Gastroenterol 46(6) Pages: 411-423
  • Peer Reviewed
• [Journal Article] Transcriptomic comparison of human hepatoma Huh-7 cellclones with different hepatitis C virus replication efficiencies (2007)
  • Author(s): Inoue Y, Murakami K. Hmwe SS, Aiaki H, Suzuki T.
  • Journal Title: Jpn J Infect Dis.60(4):173-8 60(4) Pages: 173-178
  • Peer Reviewed
• [Journal Article] The E6AP ubiquitin ligase mediates ubiquitylation and degradation of hepatitis Cvirus core protein (2007)
  • Author(s): Masayuki Shirakura, Kyoko Murakami, Tohru Ichkmura, Ryosuke Suzuki, Tetsu Shimoji, Kouichirou Fukuda, Katsutoslu Abe, Shigeko Sato, Masayoshi Fukasawa, Yoshio Yamakawa, Masahiro Nishijima, Kohji Moriishi, Yoshiharu Matsuura, Takaji Wakita, Tetsuro Suzuki;
  • Journal Title: J.Virol 81(3) Pages: 1171-1185
  • Peer Reviewed
• [Presentation] HCV慢性感染患者におけるB細胞リンパ腫発症機序の解明 : 末梢血B細胞でのHCV感染増殖およびAID (activation-induced cytidine deaminase)の発現上昇 (2008)
  • Author(s): 伊藤昌彦, 持田恵子, 村上恭子, 鈴木哲朗, 山口一成, 水落利明
  • Organizer: 第56回日本ウイルス学会学術集会
  • Place of Presentation: 岡山
  • Year and Date: 2008-10-26
• [Presentation] Ubiquitylation signal of hepatitis C virus core protein (2008)
  • Author(s): Ikuo shoji, Motonao Osaki, Kyoko Murakami, Tetsuro Suzuki, Tatsuo Miyamura, Takaji Wakita, and Hak Hotta
  • Organizer: 15th International Symposium on Hepatitis C Virus and Related Viruses
  • Place of Presentation: サンアントニオ
  • Year and Date: 2008-10-07
• [Presentation] Identification of hnRNPHl and hnRNPF as binding protein partners for HCV core protein (2008)
  • Author(s): Katsutoshi Abe, Kyoko Murakami, Satoshi Takamiya, Tetsuro Suzuki, Tatsuo Miyamura, Kazuhiko Koike, Takaji Wakita and Tetsuro Suzuki
  • Organizer: 15th International Symposium on Hepatitis C Virus and Related Viruses
  • Place of Presentation: サンアントニオ
  • Year and Date: 2008-10-06
• [Presentation] Identification of hnRNPHl as a binding partner of hepatitis C virus core protein and the IRES IIId region of viral RNA (2008)
  • Author(s): Kyoko Murakami, Katsutoshi Abe, Ikuo Shoji, Satoshi Takamiya, Toshiro Kimura, Koji Ishii, Ryosuke Suzuki, Tetsuro Suzuki, Tatsuo Miyamura, and Takaji Wakita
  • Organizer: IUMS2008(国際ウイルス会議)
  • Place of Presentation: イスタンブール
  • Year and Date: 2008-08-12
• [Presentation] Molecular mechanism of E6AP-mediated regulation of hepatitis C virus productio n. (2008)
  • Author(s): Ikuo Shoji, Motonao Osaki, Kouichirou Fukuda, Kyoko Murakami, Hak Hotta, Tetsuro Suzuki, Takaji Wakita, and Tatsuo Miyamura.
  • Organizer: keyston symposia
  • Place of Presentation: バンクーバー
  • Year and Date: 2008-04-12
• [Presentation] olecular mechanism of E6AP-mediated regulation of hepatitis C virus production. (2008)
  • Author(s): Ikuo Shoji, Motonao Osaki, Kouichirou Fukuda, Kyoko Murakami, Hak Hotta, Tetsuro Suzuki, Takaji Wakita, Tatsuo Miyamura.
  • Organizer: keystone symposia
  • Place of Presentation: バンクーバー
• [Presentation] Identification of hnRNPH1 as a binding partner of hepatitis C virus core protein and the IRES IIId region of viral RNA (2008)
  • Author(s): Kyoko Murakami, Katsutoshi Abe, Ikuo Shoji, Satoshi Takamiya, Toshiro Kimura, Koji Ishii, Ryosuke Suzuki, Tetsuro Suzuki, Tatsuo Miyamura, Takaji Wakita
  • Organizer: IUMS2008(国際ウイルス会議)
  • Place of Presentation: イスタンブール
• [Presentation] Requirement of Apoptin for CAV replication (2008)
  • Author(s): Afieono Agung Prasetyo, Toshio Kamahora, Kyoko Murakami, Shigeo Hino
  • Organizer: IUMS2008(国際ウイルス会議)
  • Place of Presentation: イスタンブール
• [Presentation] ERGIC-53 regulates the HCV RNA replication through interaction with the viral NS3 protein. (2008)
  • Author(s): Itsuki Hamamoto, Kyoko Murakami, Tetsuro Suzuki, Keiko Taya, Nobuhiko Okabe, Takaji Wakita, Ikuo Shoji
  • Organizer: 15th International Symposium on Hepatitis C Virus and Related Viruses
  • Place of Presentation: サンアントニオ
• [Presentation] Identification of hnRNPH1 and hnRNPF as binding protein partners for HCV core protein (2008)
  • Author(s): Katsutoshi Abe, Kyoko Murakami, Satoshi Takamiya, Tetsuro Suzuki, Tatsuo Miyamura, Kazuhiko Koike, Takaji Wakita, Tetsuro Suzuki
  • Organizer: 15th International Symposium on Hepatitis C Virus and Related Viruses
  • Place of Presentation: サンアントニオ
• [Presentation] Trans-encapsidation of HCV subgenomic replicon RNA with viral structure proteins (2008)
  • Author(s): Koji Ishii, Kyoko Murakami, Su Su Hmwe, Jin Li, Ryosuke Suzuki, Tatsuo Miyamura, Takaji Wakita, Tetsuro Suzuki
  • Organizer: 15th International Symposium on Hepatitis C Virus and Related Viruses
  • Place of Presentation: サンアントニオ
• [Presentation] The C-terminal serine cluster of NS5A is determinant of NS5A-core protein interaction and HCV production (2008)
  • Author(s): Hiromichi Hara, Hideki Aizaki, Mami Matsuda, Kyoko Murakami, Ikuo Shoji, Tatsuo Miyamura, Takaji Wakita, Tetsuro Suzuki
  • Organizer: 15th International Symposium on Hepatitis C Virus and Related Viruses
  • Place of Presentation: サンアントニオ
• [Presentation] Ubiquitylation signal of hepatitis C virus core protein (2008)
  • Author(s): Ikuo shoji, Motonao Osaki, Kyoko Murakami, Tetsuro Suzuki, Tatsuo Miyamura, Takaji Wakita, Hak Hotta
  • Organizer: 5th International Symposium on Hepatitis C Virus and Related Viruses
  • Place of Presentation: サンアントニオ
• [Presentation] C型肝炎ウイルス複製を制御する宿主因子ERGIC-53の機能 (2008)
  • Author(s): 浜本いつき、村上恭子、鈴木哲郎、多屋馨子、岡部信彦、脇田隆字、勝二郁夫
  • Organizer: 第56回日本ウイルス学会学術集会
  • Place of Presentation: 岡山
• [Presentation] HCVコア蛋白に結合する新規宿主因子hnRNPH1/H2のHCV生活環における役割 (2008)
  • Author(s): 村上恭子、阿部克俊、高宮智史、木村敬郎、鈴木哲朗、宮村達男、小池和彦、脇田隆字、勝二郁夫
  • Organizer: 第56回日本ウイルス学会学術集会
  • Place of Presentation: 岡山
• [Presentation] creatine Kinase BはC型肝炎ウイルスNS4Aとの相互作用によりウイルスゲノム複製複合体へ運ばれエネルギー供給に働く (2008)
  • Author(s): 原弘道、相崎英樹、松田麻未、村上恭子、勝二郁夫、松浦善治, 宮村達男、脇田隆字、鈴木哲朗
  • Organizer: 第56回日本ウイルス学会学術集会
  • Place of Presentation: 岡山
• [Presentation] C型肝炎ウイルスのsubgenomic repliconを持つウイルス様粒子の形成と感染性 (2008)
  • Author(s): 石井孝司、村上恭子、ススムエー、張斌、李津、白倉雅之、森川賢一、鈴木亮介, 宮村達男、脇田隆字、鈴木哲朗
  • Organizer: 第56回日本ウイルス学会学術集会
  • Place of Presentation: 岡山
• [Presentation] HCV粒子形成におけるNS5A蛋白の役割 (2008)
  • Author(s): 政木隆博、鈴木亮介、村上恭子、相崎英樹、石井孝司、村山麻子、伊達朋子、松浦善治、宮村達男、脇田隆字、鈴木哲朗
  • Organizer: 第56回日本ウイルス学会学術集会
  • Place of Presentation: 岡山
• [Presentation] HCV慢性感染患者におけるB細胞リンパ腫発症機序の解明:末梢血B細胞でのHCV感染増殖およびAID (activation-induced cytidine deaminase)の発現上昇 (2008)
  • Author(s): 伊藤昌彦、持田恵子、村上恭子、鈴木哲朗、山口一成、水落利明
  • Organizer: 第56回日本ウイルス学会学術集会
  • Place of Presentation: 岡山
• [Presentation] HCVコア蛋白に結合する新規宿主因子hnRNPH1/H2のHCV生活環における役割 (2008)
  • Author(s): 村上恭子、阿部克俊、高宮智史、木村敬郎、鈴木哲朗、宮村達男、小池和彦、脇田隆字、勝二郁夫
  • Organizer: 第31回日本分子生物学会年会
  • Place of Presentation: 神戸
• [Presentation] C型肝炎ウイルスコア蛋白質のユビキチン化シグナル (2008)
  • Author(s): 勝二郁夫、大崎一直、村上恭子、鈴木哲朗、宮村達男、脇田隆字、堀田博
  • Organizer: 第31回日本分子生物学会年会
  • Place of Presentation: 神戸
• [Presentation] Virological characterization of.HCV JFH-1 stain in B-lymphocytes (2007)
  • Author(s): Murakami K., Shoji I., Hamamoto I., Suzuki T., Miyamura T., Wakita T.
  • Organizer: 14th International Symposium on Hepatitis C Virus and Related Viruses
  • Place of Presentation: イギリス(グラスゴー)
  • Year and Date: 2007-09-10
• [Presentation] Celluar vimentin content affects the protein level of hepatitis C virus core protei n and the activity of hepatitis C virus production in cultured cells (2007)
  • Author(s): Fukasawa M., Nitahara-Kasahara Y., Shinkai-Ouchi F., Sato S., Suzuki T., Murakami K., Wakita T., Hanada K Miyamura T., Mishijima M.
  • Organizer: 14th International Symposium on Hepatitis C Virus and Related Viruses
  • Place of Presentation: イギリス(グラスゴー)
  • Year and Date: 2007-09-10
• [Presentation] 血球系細胞におけるHCVJFH-1株の感染および複製の検討 (2007)
  • Author(s): 村上 恭子、勝二 郁夫、木村 敬郎、鈴木 哲朗、宮村 達男、脇田 隆字
  • Organizer: 血球系細胞におけるHCVJFH-1株の感染および複製の検討
  • Place of Presentation: 札幌
• [Presentation] HCVコア蛋白と結合する新規宿主因子kmRNPHIIH2の同定と相互作用解析 (2007)
  • Author(s): 阿部 克俊、勝二 郁夫、村上 恭子、奈須 純一、高宮 智史、大崎 一直、鈴木 哲朗、宮村 達男、小池 和彦、脇田 隆字
  • Organizer: 第55回日本ウイルス学会学術集会
  • Place of Presentation: 札幌