Functional analysis of Vasohibin-binding proteins

• Project/Area Number: 19790509
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Circulatory organs internal medicine
• Research Institution: Tohoku University
• Principal Investigator: SUZUKI Yasuhiro Tohoku University, 加齢医学研究所, 産学官連携研究員 (60332277)
• Project Period (FY): 2007 – 2009
• Project Status: Completed (Fiscal Year 2009)
• Budget Amount: ¥3,830,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥630,000); Fiscal Year 2009: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2008: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2007: ¥1,100,000 (Direct Cost: ¥1,100,000)
• Keywords: 血管新生 / 内皮細胞 / 蛋白質分泌 / 細胞遊走 / 微小管 / チューブリン / ユビキチン化 / プロテアソーム / 抗血管新生

Research Abstract

In this study, the physiological functions of two Vasohibin-binding proteins were analyzed. A small Vasohibin-binding protein (SVBP) interacted with Vasohibin within vascular endothelial cell and enhanced the extracellular secretion of their complex. TMEM16F affected the posttranscriptional modification of α-tubulin induced by Vasohibin. These results suggested that SVBP and TMEM16F might contribute to the anti-angiogenic activity of Vasohibin.

Research Products

• [Journal Article] Isolation of a small vasohibin-binding protein (SVBP) and its role in vasohibin secretion. (2010)
  • Author(s): Suzuki Y, Kobayashi M, Miyashita H, Ohta H, Sonoda H, Sato Y
  • Journal Title: Journal of Cell Science (in press)
  • Peer Reviewed
• [Journal Article] Endogenous Angiogenesis Inhibitor Vasohibin1 Exhibits Broad-Spectrum Antilymphangiogenic Activity and Suppresses Lymph Node Metastasis. (2010)
  • Author(s): Heishi T, Hosaka T, Suzuki Y, Miyashita H, Oike Y, Takahashi T, Nakamura T, Arioka S, Mitsuda Y, Takakura T, Hojo K, Matsumoto M, Yamauchi C, Ohta H, Sonoda H, Sato Y
  • Journal Title: The American Journal of Pathology 176 Pages: 1950-1958
  • Peer Reviewed
• [Journal Article] Endogenous Angiogenesis Inhibitor Vasohibin1 Exhibits Broad-Spectrum Antilymphangiogenic Activity and Suppresses Lymph Node Metastasis (2010)
  • Author(s): Heishi T, Hosaka T, Suzuki Y, Miyashita H, Oike Y, Takahashi T, Nakamura T, Arioka S, Mitsuda Y, Takakura T, Hojo K, Matsumoto M, Yamauchi C, Ohta H, Sonoda H, Sato Y.
  • Journal Title: The American Journal of Pathology 176 Pages: 1950-1958
  • Peer Reviewed
• [Journal Article] Vasohibin-1 expression in endothelium of tumor blood vessels regulates angiogenesis. (2009)
  • Author(s): Hosaka T, Kimura H, Heishi T, Suzuki Y, Miyashita H, Ohta H, Sonoda H, Moriya T, Suzuki S, Kondo T, Sato Y
  • Journal Title: The American Journal of Pathology 175 Pages: 430-439
  • Peer Reviewed
• [Journal Article] Distinctive localization and opposed roles of vasohibin-1 and vasohibin-2 in the regulation of angiogenesis (2009)
  • Author(s): Kimura H, Miyashita H, Suzuki Y, Kobayashi M, Watanabe K, Sonoda H, Ohta H, Fujiwara T, Shimosegawa T, Sato Y
  • Journal Title: Blood 113 Pages: 4810-4818
  • Peer Reviewed
• [Journal Article] Vasohibin-1 expression in endothelium of tumor blood vessels regulates angiogenesis (2009)
  • Author(s): Hosaka T, Kimura H, Heishi T, Suzuki Y, Miyashita H, Ohta H, Sonoda H, Moriya T, Suzuki S, Kondo T, Sato Y.
  • Journal Title: The American Journal of Pathology 175 Pages: 430-439
  • Peer Reviewed
• [Journal Article] Distinctive localization and opposed roles of vasohibin-1 and vasohibin-2 in the regulation of angiogenesis (2009)
  • Author(s): Kimura H, Miyashita H, Suzuki Y, Kobayashi M, Watanabe K, Sonoda H, Ohta H, Fujiwara T, Shimosegawa T, Sato Y.
  • Journal Title: Blood 113 Pages: 4810-4818
  • Peer Reviewed
• [Journal Article] Vasohibin-1 expressed in endothelium of tumor vessels regulates angiogenesis (2009)
  • Author(s): Hosaka T, Kimura H, Heishi T, Suzuki Y, Miyashita H, Ohta H, Sonoda H, Moriya T, Suzuki S, Kondo T, Sato Y
  • Journal Title: The American Journal of Pathology (accepted for publication)
  • Peer Reviewed
• [Journal Article] Distinctive localization and opposed roles of vasohibin-1 and vasohibin-2 in the regulation of angiogenesis (2009)
  • Author(s): Kimura H, Miyashita H, Suzuki Y, Kobayashi M, Watanabe K, Sonoda H, Ohta H, Fujiwara T, Shimosegawa T, Sato Y
  • Journal Title: Blood (Epub ahead of print)
  • Peer Reviewed
• [Presentation] 血管新生抑制因子Vasohibin-1はチューブリンの脱チロシン化を誘導することで内皮細胞の遊走阻害を引き起こす (2010)
  • Author(s): 小林美穂、鈴木康弘、佐藤靖史
  • Organizer: 第17回日本血管生物医学会学術大会
  • Place of Presentation: 東京
• [Presentation] Vasohibin-1 induced detyrosination of α-tubulin triggers an inhibition of endothelial cell migration (2010)
  • Author(s): 小林美穂、鈴木康弘、佐藤靖史
  • Organizer: 第68回日本癌学会学術総会
  • Place of Presentation: 横浜
• [Presentation] Isolation of a small Vasohibin-binding protein (SVBP) and its important role in the secretion of Vasohibin (2009)
  • Author(s): 鈴木康弘、小林美穂、宮下浩輝、太田英樹、園田光、佐藤靖史
  • Organizer: 第16回日本血管生物医学会学術大会・The 6th Korea-Japan Symposium on Vascular Biology
  • Place of Presentation: 金沢
• [Presentation] Vasohibin結合性低分子量蛋白SVBPの単離と分泌における役割 (2009)
  • Author(s): 鈴木康弘、小林美穂、宮下浩輝、太田英樹、園田光、佐藤靖史
  • Organizer: 67回日本癌学会学術総会
  • Place of Presentation: 名古屋
• [Presentation] 血管新生抑制因子 Vasohibin-1はチューブリンの脱チロシン化を誘導することで内皮細胞の遊走阻害を引き起こす (2009)
  • Author(s): 小林美穂、鈴木康弘、佐藤靖史
  • Organizer: 第17回日本血管生物医学会
  • Place of Presentation: 東京
• [Presentation] Vasohibin結合性低分子量蛋白SVBPの単離と分泌における役割 (2008)
  • Author(s): 鈴木康弘、小林美穂、宮下浩輝、太田英樹、園田光、佐藤靖史
  • Organizer: 第67回日本癌学会学術総会
  • Place of Presentation: 名古屋
• [Presentation] Isolation of a small Vasohibin-binding protein(SVBP)and its important role in the secretion of Vasohibin (2008)
  • Author(s): 鈴木康弘、小林美穂、宮下浩輝、太田英樹、園田光、佐藤靖史
  • Organizer: 第16回日本血管生物医学会学術大会・The 6th Korea Japan Joint Symposium on Vascular Biology合同学術大会
  • Place of Presentation: 金沢
• [Book] 実験医学増刊シグナル伝達研究2008-'09第2章-11 Vasohibinによる血管新生のネガティブフィードバック調節 (2008)
  • Author(s): 鈴木康弘、佐藤靖史
  • Publisher: 羊土社
• [Book] 実験医学増刊シグナル伝達研究2008-09(第2章-11 : Vasohibinによる血管新生のネガティブフィードバック調節) (2008)
  • Author(s): 鈴木康弘、佐藤靖史
  • Publisher: 羊土社
• [Remarks]
  • URL: http://www.idac.tohoku.ac.jp/dep/vascbio/
• [Remarks]
  • URL: http://www.idac.tohoku.ac.jp/dep/vascbio/
• [Remarks]
  • URL: http://www.idac.tohoku.ac.jp/dep/vascbio/
• [Remarks]
  • URL: http://www.idac.tohoku.ac.jp/dep/vascbio/