Mechanistic Insights into the Association between Autophagy and Progression from Compensated Cardiac Hypertrophy to Heart Failure

• Project/Area Number: 19790512
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Circulatory organs internal medicine
• Research Institution: Akita University
• Principal Investigator: NOBORI Kiyoshi Akita University, 医学部, 特任講師 (40436192)
• Project Period (FY): 2007 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥3,560,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥360,000); Fiscal Year 2008: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2007: ¥2,000,000 (Direct Cost: ¥2,000,000)
• Keywords: 心臓病態学 / オートファジー / 遺伝子組み換えマウス / 心筋細胞

Research Abstract

ライソゾームと融合後にも分解されにくく、細胞毒性が少ないmRFPの改良赤色蛍光蛋白mCherryを使用し、心筋特異的プロモーターαMHCを用いて、我々は、心筋細胞にてオートファジーを評価するための心筋特異的にmCherry-LC3を発現するトランスジェニックマウスを作製した。GFP-LC3-TGとのダブルトランスジェニックを作成し、オートファゴゾームとオートライソゾームとを区別して認識するマウスを確立した。

Research Products

• [Journal Article] ATF3 protects against renal ischemia-reperfusion injury. (2008)
  • Author(s): Yoshida T, Sugiura H, Mitobe M, Tsuchiya K, Shirota S, Nishimura S, Shiohira S, Ito H, Nobori K, Gullans SR, Akiba T, Nitta K .
  • Journal Title: J Am Soc Nephrol. 19(2) Pages: 217-24
  • Peer Reviewed
• [Journal Article] ATF3 protects against renal ischemia-reperfusion injury. (2008)
  • Author(s): Yoshida, T
  • Journal Title: J Am Soc Nephrol. 19(2) Pages: 217-24
  • Peer Reviewed
• [Journal Article] Functional role of stromal interaction molecule 1 (STIM1) in vascular smooth muscle cells. (2007)
  • Author(s): Takahashi Y, Watanabe H, Murakami M, Ono K, Munehisa Y, Koyama T, Nobori K, Iijima T, Ito H.
  • Journal Title: Biochem Biophys Res Commun. 361(4) Pages: 934-40
  • Peer Reviewed
• [Journal Article] Intracellular signaling pathways for cardiac hypertrophy: ERK, JAK-STAT, S6 kinase (2007)
  • Author(s): Nobori K, Munehisa Y. Ito H.
  • Journal Title: Nippon Rinsho 65 Suppl 4 Pages: 196-200
  • NAID: 40015438302
• [Journal Article] Essential role of the N-terminus of murine Orai1 in store-operated Ca2+ entry. (2007)
  • Author(s): Takahashi Y, Murakami M, Watanabe H, Hasegawa H, Ohba T, Munehisa Y, Nobori K, Ono K, Iijima T, Ito H.
  • Journal Title: Biochem Biophys Res Commun. 356(1) Pages: 45-52
  • Peer Reviewed
• [Journal Article] Functional role of stromal interaction molecule 1 in vascular smooth muscle cells. (2007)
  • Author(s): Takahashi, Y
  • Journal Title: Biochem Biophys Res Commun. 361(4) Pages: 934-40
  • Peer Reviewed
• [Journal Article] Intracellular singnaling pathways for cardiac hypertrophy:ERK, JAK-STAT, S6 kinase (2007)
  • Author(s): Nobori, K
  • Journal Title: Nippon Rinsho 65 Suppl 4 Pages: 196-200
• [Presentation] The Percentage of Angiogenic T Cells in Peripheral Blood was Increased in the Patients with Coronary Artery Disease (2009)
  • Author(s): 柿崎学
  • Organizer: 第73回日本循環器学会総会
  • Place of Presentation: 大阪
  • Year and Date: 2009-03-20
• [Presentation] The Percentage of Angiogenic T Cells in Peripheral Blood was Increased in the Patients with Coronary Artery Disease. (2009)
  • Author(s): 柿崎学、野堀 潔、伊藤宏
  • Organizer: 第 73 回日本循環器学会総会
• [Presentation] α MyHC mCherry LC3 Transgenic Mouse is a New and Useful Tool to Examine the Role of Autophagy in Cardiomyocytes (2008)
  • Author(s): 寺田舞
  • Organizer: American Heart Association scientific meeting
  • Place of Presentation: アメリカ、ニューオリンズ
  • Year and Date: 2008-11-09
• [Presentation] a MyHC mCherry LC3 Transgenic Mouse is a New and Useful Tool to Examine the Role of Autophagy in Cardiomyocytes (2008)
  • Author(s): 寺田舞
  • Organizer: 第72回日本循環器学会総会
  • Place of Presentation: 福岡県博多市
  • Year and Date: 2008-03-29
• [Presentation] αMyHC mCherry LC3 Transgenic Mouse is a New and Useful Tool to Examine the Role of Autophagy in Cardiomyocytes (2008)
  • Author(s): 寺田舞、野堀 潔、伊藤宏
  • Organizer: American Heart Association scientific meeting
• [Presentation]
  • Author(s): 柿崎学、野堀 潔、伊藤宏
• [Book] meditina循環器病薬の使い方2009 (2009)
  • Author(s): 野堀 潔、柿崎学、伊藤宏
  • Publisher: 医学書院
• [Book] meditina循環器病薬の使い方2009 (2009)
  • Author(s): 野堀潔
  • Publisher: 医学書院
• [Book] 心腎相関の病態理解と診療 (2008)
  • Author(s): 野堀 潔、伊藤宏
  • Publisher: 羊土社
• [Book] 心腎相関の病態理解と診療 (2008)
  • Author(s): 野堀潔
  • Publisher: 羊土社