Role of small G protein Rad in diabetic cardiomyopathy

• Project/Area Number: 19790541
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Circulatory organs internal medicine
• Research Institution: Keio University
• Principal Investigator: MURATA Mitsushige Keio University, 医学部, 講師 (30317135)
• Research Collaborator: YAMAKAWA Hiroyuki 慶應義塾大学, 医学部, 助教 (80465020)
• Project Period (FY): 2007 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥3,660,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥360,000); Fiscal Year 2008: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2007: ¥2,100,000 (Direct Cost: ¥2,100,000)
• Keywords: 低分子量G蛋白質 / カルシウムチャネル / 細胞内カルシウム濃度

Research Abstract

低分子量G蛋白質Radは心筋L型カルシウムチャネル発現調節に加えて、細胞内プロテインキナーゼAを調節することにより間接的に筋小胞体のリアノジン受容体の活性を修飾していることが明らかとなった。これらの結果より、糖尿病性心筋症におけるRad発現増加は、同疾患における心機能低下の原因の一つである可能性がある。

Research Products

• [Journal Article] Glucocorticoid protects heart from ischemia-reperfusion injury through activation of lipocalin-type prostaglandin D synthase-derived PGD2 biosynthesis (2009)
  • Author(s): Tokudome S, Sano M, Shinmura K, Matsuhashi T, Morizane S, Moriyama H, Tamaki K, Nakanishi H, Yoshikawa N, Shimizu N, Endo J, Katayama T, Murata M, Yuasa S, Kaneda R, Tomita K, Eguchi N, Urade Y, Asano K, Utsunomiya Y, Suzuki T, Taguchi R, Tanaka H, Fukuda K.
  • Journal Title: J Clin Invest (in press)
  • Peer Reviewed
• [Journal Article] Left ventricular pseudoaneurysm with peculiar coronary artery collpase. (2009)
  • Author(s): Katayama T, Murata M, Iwanaga S, Kawamura A, Yoshikawa T, Ogawa S.
  • Journal Title: J Am Coll Cardiol (in press) Pages: 122-122
  • Peer Reviewed
• [Journal Article] (2009)
  • Author(s): Hyodo M, Makino S, Awaji Y, Sakurada Y, Ohkubo T, Murata M, Fukuda K, Tsuda M.
  • Journal Title: Int J Dev Biol (in press)
  • Peer Reviewed
• [Journal Article] Glucocorticoid protects heart from ischemiareperfusion injury through activation of lipocalin-type prostaglandin D synthasederived PGD2 biosynthesis (2009)
  • Author(s): Tokudome S, Sano M, Shinmura K, Matsuhashi T, Morizane S, Moriyama H, Tamaki K, Nakanishi H, Yos hikawa N, Shimizu N, Endo J, Katayama T, Murata M, Yuasa S, Kaneda R, Tomita K, Eguchi N, Urade Y, Asano K, Utsunomiya Y, Suzuki T, Tacuchi R. Tanaka H, Fukuda K
  • Journal Title: J Clin Invest (in press)
  • Peer Reviewed
• [Journal Article] Left ventricular pseudoaneurysm with peculiar coronary artery collpase (2009)
  • Author(s): Katayama T, Murata M, Iwanaga S, Kawamura A, Yoshikawa T, Ogawa S
  • Journal Title: J Am Coll Cardiol (in press)
  • Peer Reviewed
• [Journal Article] A novel in vitro system for cardiomyocyte differentiation with embryonic cells of the medaka (Oryzias latipes) (2009)
  • Author(s): Hyodo M, Makino S, Awaji Y, Saku rada Y, Ohkubo T, Murata M, Fukuda K, Tsuda M
  • Journal Title: Int J Dev Biol (in press)
  • Peer Reviewed
• [Journal Article] Common marmoset embryonic stem cell can differentiate into cardiomyocytes. (2008)
  • Author(s): Chen H, Hattori F, Murata M, Li W, Yuasa S, Onitsuka T, Shimoji K, Ohno Y, Sasaki E, Kimura K, Hakuno D, Sano M, Makino S, Ogawa S, Fukuda K.
  • Journal Title: Biochem Biophys Res Commun. 369 Pages: 801-806
  • Peer Reviewed
• [Journal Article] A real-time 3-dimensional echocardiographic quantitative analysis of left atrial function in left ventricular diastolic dysfunction (2008)
  • Author(s): Murata M, Iwanaga S, Tamura Y, Kondo M, Kouyama K, Murata M, Ogawa S
  • Journal Title: Am J Cardiol 102 Pages: 1097-1102
  • Peer Reviewed
• [Journal Article] Common marmoset embryonic stem cell can differentiate into cardiomyocytes (2008)
  • Author(s): Chen H, Hattori F, Murata M, Li W, Yuasa S, Onitsuka T, Shimoji K, Ohno Y, Sasaki E, Kimura K, Hakuno D, Sano M, Makino S, Ogawa S, Fukuda K
  • Journal Title: Biochem Biophys Res Commun 369 Pages: 801-806
  • Peer Reviewed
• [Journal Article] A real-time 3dimensional echocardiographic quantitative analysis of left atrial function in left ventricular diastolic dysfunction (2008)
  • Author(s): Murata M, Iwanaga S, Tamura Y, Kondo M, Kouyama K, Murata M, Ogawa S
  • Journal Title: Am J Cardiol 102 Pages: 1097-1102
  • Peer Reviewed
• [Journal Article] Dominant negative suppression of Rad leads to QT prolongation and causes ventricular arrhythmias via modulation of L-type Ca channels in the heart (2007)
  • Author(s): Yada H, Murata M., et. al.
  • Journal Title: Circulation Research 101 Pages: 69-77
  • Peer Reviewed
• [Presentation] Regulation of sarcoplasmic reticulum Ca2+ leak by small G protein Rad in the heart (2009)
  • Author(s): Murata M, Yamakawa H, Yada H, Makino S, Sano M, Ogawa S, Fukuda K
  • Organizer: 第73回日本循環器学会学術集会(シンポジウム)
  • Place of Presentation: 大阪
  • Year and Date: 2009-03-22
• [Presentation] Cardiomyocytes derived from bimaternal parthenogenetic ES cells by epigenetic modification have a different property and genetic presentation in cardiogenesis (2009)
  • Author(s): Kageyama Toshimi
  • Organizer: 第73回日本循環器学会学術集会
  • Place of Presentation: 大阪
  • Year and Date: 2009-03-22
• [Presentation] Regulation of sarcoplasmic reticulum Ca^<2+> leak by small G protein Rad in the heart (2009)
  • Author(s): Murata Mitsushige
  • Organizer: 第73回日本循環器学会学術集会
  • Place of Presentation: 大阪
  • Year and Date: 2009-03-22
• [Presentation] Kinase-dependent metabolic shift towards Phgdh-mediated serine synthesis enhances cardioprotection to oxidative stress as a hermetic responses to aldehydes (2009)
  • Author(s): Endo Jin
  • Organizer: 第73回日本循環器学会学術集会
  • Place of Presentation: 大阪
  • Year and Date: 2009-03-21
• [Presentation] Neural crest stem cels supply intrinsic cardiac adrenergic cells and contribute to reinnervation after myocardial infarction in mice (2009)
  • Author(s): Tamura Yuichi
  • Organizer: 第73回日本循環器学会学術集会
  • Place of Presentation: 大阪
  • Year and Date: 2009-03-21
• [Presentation] Dominant negative suppression of Rad leads to intracellular Ca2+ overload via up-regulation of cardiac ryanodine receptor activity (2008)
  • Author(s): Murata M, Yada H, Yamakawa H, Murata M, Ogawa S, Fukuda K
  • Organizer: The 25th the Annual Meeting of International Society for Heart Research Japanese Section(ISHR)
  • Place of Presentation: 横浜
  • Year and Date: 2008-12-05
• [Presentation] Dominant negative suppression of Rad leads to intracellular Ca^<2+> overload via up-regulation of cardiac rvanodine receptor activity (2008)
  • Author(s): Murata Mitsushige
  • Organizer: The 25^<th> the Annual Meeting of International Society for Heart Research Japanese Section
  • Place of Presentation: 横浜
  • Year and Date: 2008-12-05
• [Presentation] A common SCN5A polymorphism associated with severe QT prolongation and Torsades de Pointes development after thyroidectomy in a patient with Graves'disease (2008)
  • Author(s): Aizawa Y, Murata M, Muraki H, Miyoshi S, Yoshikawa T, Fukuda K, Ogawa S
  • Organizer: 1st Asia-Pacific Heart Rhythm Society Session(APHRS)
  • Place of Presentation: Singapore
  • Year and Date: 2008-11-29
• [Presentation] A common SCN5A polymorphism associated with severe QT prolongation and Torsades de Pointes development after thyroidectomy in a patient with Graves' disease (2008)
  • Author(s): Aizawa Yoshiyasu
  • Organizer: 1^<st> Asia-Pacific Heart Rhythm Society Session
  • Place of Presentation: Singapore
  • Year and Date: 2008-11-29
• [Presentation] Dominant negative suppression of Rad leads to intracellular Ca2+ overload via up-regulation of cardiac ryanodine receptor activity (2008)
  • Author(s): Murata M, Yada H, Yamakawa H, Aizawa Y, Yuasa S, Hakuno D, Makino S, Sano M, Murata M, Ogawa S, Fukuda K
  • Organizer: 81st American Heart Association scientific meeting
  • Place of Presentation: New Orleans, USA
  • Year and Date: 2008-11-13
• [Presentation] Quantitative analysis of right ventricular function in patients with pulmonary hypertension : three-dimensional echocardiographic study (2008)
  • Author(s): Morikawa T, Murata M, Okuda S, Iwanaga S, Satoh T, Kohsaka S, Nakamizo H, Suzuki K, Matsushita K, Hakuno D, Murata M, Ogawa S
  • Organizer: 81st American Heart Association scientific meeting
  • Place of Presentation: New Orleans, USA
  • Year and Date: 2008-11-13
• [Presentation] The potent angiogenic factor periostin accelerates degeneration and sclerosis of the cardiac valve complex (2008)
  • Author(s): Hakuno D, Kimura N, Kimura T, Yuasa S, Murata M, Makino S, Sano M, Okada Y, Yozu R, Kudo A, Ogawa S, Fukuda K
  • Organizer: 81st American Heart Association scientific meeting
  • Place of Presentation: New Orleans, USA
  • Year and Date: 2008-11-12
• [Presentation] Imprinting gene-modified parthenogenic ES cells can be a novel autologous cell source for generating regenerative cardiomyocytes (2008)
  • Author(s): Kageyama T, Makino S, Hattori F, Onizuka T, yun S, Ohno Y, Endo J, Shimoji K, Arai T, Hakuno D, Tanaka T, Kimura K, Hayashida K, Murata M, Katayama T, Sano M, Tokunaga T, Kono T, Ogawa S, Fukuda K
  • Organizer: 81st American Heart Association scientific meeting
  • Place of Presentation: New Orleans, USA
  • Year and Date: 2008-11-11
• [Presentation] Dominant negative suppression of Rad leads to QT prolongation and causes ventricular arrhythmias via modulation of L-type Ca2+ channels in the heart (2008)
  • Author(s): Yada H, Murata M, Shimoda K, Yuasa S, Kawaguchi H, Ieda M, Adachi T, Murata M, Ogawa S, Fukuda K
  • Organizer: 第25回日本心電学会学術集会
  • Place of Presentation: 新潟
  • Year and Date: 2008-11-11
• [Presentation] Imprinting gene-modified parthenogenic ES cells can be a novel autologous cell source for eeneratina reaenerative cardiomvocvtes (2008)
  • Author(s): Kageyama Toshimi
  • Organizer: 81st American Heart Association scientific meeting
  • Place of Presentation: New Orleans, USA
  • Year and Date: 2008-11-11
• [Presentation] Dominant negative suppression of Rad leads to QT prolongation and causes ventricular arrhythmias via modulation of L-type Ca^<2+> channels in the heart (2008)
  • Author(s): Yada Hirotaka
  • Organizer: 第25回日本心電学会学術集会
  • Place of Presentation: 新潟
  • Year and Date: 2008-11-02
• [Presentation] Regulation of intracellular Ca2+ homeostasis by a ras-related small G-protein Rad in the heart (2008)
  • Author(s): Murata M, Yada H, Yamakawa H, Murata M, Ogawa S, Fukuda K
  • Organizer: The International Discussion Forum & the 242nd Tokyo Discussion Meeting of the Physiological Society of Japan
  • Place of Presentation: 東京
  • Year and Date: 2008-10-26
• [Presentation] Regulation of intracellular Ca2+ homeostasis by a ras-related small G-protein Rad in the heart (2008)
  • Author(s): Murata Mitsushige
  • Organizer: The International Discussion Forum & the 242^<nd> Tokyo Discussion Meeting of the Physiological Society of Japan
  • Place of Presentation: 東京
  • Year and Date: 2008-10-26
• [Presentation] Deregulation of small G-protein Rad affects L-type Ca^<2+>channel function and Ca^<2+>homeostasis by a subunit-traffcking in heart failure (2008)
  • Author(s): Murata M
  • Organizer: 第72回日本循環器学会学術集会
  • Place of Presentation: 福岡
  • Year and Date: 2008-03-28