A novel biotechnology of gene therapy and human embryonic stem cells for regenerative medicine

• Project/Area Number: 19790547
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Circulatory organs internal medicine
• Research Institution: Kurume University
• Principal Investigator: TAKAHASHI Tomoyuki Kurume University, 高次脳疾患研究所, 准教授 (20332687)
• Project Period (FY): 2007 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥3,690,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥390,000); Fiscal Year 2008: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2007: ¥2,000,000 (Direct Cost: ¥2,000,000)
• Keywords: 心臓病態学 / ヒトES細胞 / 心筋発生・分化 / 再生療法 / 心筋分化・発生

Research Abstract

これまで申請者は、胚性幹(ES)細胞による難治性循環器疾患の再生医療技術開発を目指したマウスES細胞における心筋分化誘導法(Kawai-T, Takahashi-T, et al. '04Circ J.)、ならびにES細胞由来心筋分化細胞の同定・単離技術の開発(Adenoviral conditionaltargeting法)を行ってきた(Takahashi-T, et al. '06 Mol. Ther.)。本研究課題では、マウスES細胞研究において培ってきた分化誘導法や目的細胞の同定・単離技術をヒトES細胞において応用する事によって、主にアデノウイルスベクターを利用したヒトES細胞による再生医療技術開発を行なった。

Research Products

• [Journal Article] Combined therapy with cardioprotective cytokine administration and antiapoptotic gene transfer in postinfarction heart failure. (2009)
  • Author(s): Okada H., Takemura G., Kosai KI., Tsujimoto A., Esaki M., Takahashi T., Nagano S., Kanamori H., Miyata S., Li Y., Ohno T., Maruyama R., Ogino A., Li L., Nakagawa M., Nagashima K., Fujiwara T., Fujiwara H., Minatoguchi S.
  • Journal Title: Am J Physiol : Heart and Circulatory Physiology 296(3)
  • Peer Reviewed
• [Journal Article] Combined therapy with cardioprotective cytokine administration and anti-apoptotic gene transfer in postinfarction heart failure (2009)
  • Author(s): Okada M., Takemura G., Kosai K., Takahashi T., et.al.
  • Journal Title: Am J Physiol : Heart and Circulatory Physiology 296(3)
  • Peer Reviewed
• [Journal Article] Application of an adenoviral vector encoding soluble transforming growth factor-beta type II receptor to the treatment of diabetic nephropathy in mice (2008)
  • Author(s): Kondo T., Takemura G., Kosai K., Ohno T., Takahashi T., Esaki M.,Goto K., Maruyama R., Murata I., Minatoguchi S., Fujiwara T., Fujiwara H.
  • Journal Title: Clin Exp Pharmacol Physiol 35(11) Pages: 1288-1293
  • Peer Reviewed
• [Journal Article] Treatment with an adenoviral vector encoding hepatocyte growth factor mitigates established cardiac dysfunction in doxorubicin-induced cardiomyopathy (2008)
  • Author(s): Esaki M., Takemura G., Kosai K., Takahashi T., Miyata S., Li L., Goto K., Maruyama R., Okada H., Kanamori H., Ogino A., Ushikoshi H., Minatoguchi S., Fujiwara T., Fujiwara H.
  • Journal Title: Am J Physiol : Heart and Circulatory Physiology 294(2)
  • Peer Reviewed
• [Journal Article] Application of an adenoviral vector encoding soluble transforming growth factor-betatype II receptor to the treatment of diabetic nephropathy in mice (2008)
  • Author(s): Kondo T., Takemura G., Kosai K., Ohno T., Takahashi T., et.al.
  • Journal Title: Clin Exp Pharmacol Physiol. 35(11) Pages: 1288-1293
  • Peer Reviewed
• [Journal Article] Treatment with an adenoviral vector encoding hepatocyte growth factor mitigates established cardiac dysfunction in doxorubicin-induced cardiomyopathy (2008)
  • Author(s): Esaki M., Takemura G., Kosai K., Takahashi T., et.al.
  • Journal Title: Am J Physiol : Heart and Circulatory Physiology 294(2)
  • Peer Reviewed
• [Journal Article] Treatment with an adenoviral vector encoding hepatocyte growth factor mitigates est ablished cardiac dysfunction in doxorubicin-induced cardiomyopathy (2008)
  • Author(s): Esaki M., Takemure G., Kosai K., Takahashi T., et. al.
  • Journal Title: Am J Physiol: Heart and Circulatory Physiology 294(2) Pages: 1048-1057
  • Peer Reviewed
• [Journal Article] In vivo hepatocyte growth factor gene transfer reduces myocardial ischemiareperfusion injury through its multiple actions. (2007)
  • Author(s): Chen XH., Minatogichi S., Kosai K., Yuge K., Takahashi T., Arai M., Wang N., Misao Y., Lu. C., Onogi H., Kobayashi H., Yasuda S., Ezaki M., Ushikoshi H., Takemura G., Fujiwara T., Fujiwara H.
  • Journal Title: J Card Fail. 13(10) Pages: 874-883
  • Peer Reviewed
• [Journal Article] In vivo hepatocyte growth factor factor gene transfer reduces myocardial sichemia-reperfusi on injury through its multiple actions (2007)
  • Author(s): Chen XH., Minatogichi S., Kosai K., Yuge K., Takahashi T., et. al.
  • Journal Title: J Card Fail. 13(10) Pages: 874-883
  • Peer Reviewed
• [Presentation] 川崎病急性期における流血中の樹状細胞の動態Significant decrease of circulating myeloid dendritic cells in acute phase of Kawasaki disease (2008)
  • Author(s): 須田憲治、安川秀雄、高橋知之、岡村尚昌、西野裕、横山隆人、工藤嘉公、石井治佳、家村素史、松石豊次郎
  • Organizer: 第28回日本川崎病研究会
  • Place of Presentation: 札幌医科大学
• [Presentation] Conditional replicating adenovirus regulated by SURVIVIN and CER promoters exerted more strict cancer specificity and potent anti-cancer effect (2007)
  • Author(s): Murofushi Y., Kamizono J., Khai NC., Takahashi T., Komiya S.,and Kosai K.
  • Organizer: Japan Society of Gene Therapy The 13th Annual Meeting
  • Place of Presentation: International Conference Hall (Aichi Cancer Center)
• [Remarks]
  • URL: http://www.med.kurumeu.ac.jp/med/brain/index.html