New therapeutic strategy targeting thrombospondin-1 in pulmonary fibrosis

• Project/Area Number: 19790564
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Respiratory organ internal medicine
• Research Institution: Oita University
• Principal Investigator: ISHII Hiroshi Oita University, 医学部, 助教 (60398146)
• Project Period (FY): 2007 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥3,190,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥390,000); Fiscal Year 2008: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2007: ¥1,500,000 (Direct Cost: ¥1,500,000)
• Keywords: 呼吸器病学 / thrombospondin-1 / 肺線維症 / siRNA

Research Abstract

間質性肺炎群と健常人の血清および気管支肺胞洗浄液を用いてTSP-1濃度をELISA法にて測定した。血清TSP-1濃度は間質性肺炎群で高値を呈し、VEGF濃度と相関しており、本疾患の病態におけるTSP-1の重要性が示された。免疫組織化学的検討では再生肺胞上皮細胞および肺胞マクロファージの重要性が示唆された。TSP-1に対するsiRNA(short interfering RNA)を数種類作製し、気道上皮細胞を用いた実験において、一つのsiRNAで良好な抑制効果がみられるようになった。肺線維症動物モデルにおける気道へのsiRNA-TSP-1投与方法を思案しながら、抑制実験にとりかかっている。

Research Products

• [Journal Article] Desquamative interstitial pneumonia(DIP) in a patient with RA : Is DIP associated with autoimmune disorders? (2009)
  • Author(s): Ishii H, Iwata A, et al
  • Journal Title: Internal Medicine 48 Pages: 827-830
  • Peer Reviewed
• [Journal Article] Desquamative interstitial pneumonia (DIP) occurred in a patient with RA : Is DIP associated with autoimmune disorders? (2009)
  • Author(s): Ishii H, Iwata A, et.al.
  • Journal Title: Intemal Medicine 48 Pages: 827-830
  • Peer Reviewed
• [Journal Article] High serum levels of thrombospondin-1 in patients with interstitial pneumonia (2008)
  • Author(s): Ide M, Ishii H, et al
  • Journal Title: Respiratory medicine 102 Pages: 1625-1630
  • Peer Reviewed
• [Journal Article] Usual interstitial pneumonia associated with primary biliary cirrhosis (2008)
  • Author(s): Ishii H, Iwata A, et al
  • Journal Title: Respiratory medicine CME 1 Pages: 215-217
  • Peer Reviewed
• [Journal Article] High serum levels of thrombospondin-1 in patients with interstitial pneumonia (2008)
  • Author(s): Ide M, Ishii H, et.al.
  • Journal Title: Respiratory medicine 102 Pages: 1625-1630
  • Peer Reviewed
• [Journal Article] Usual interstitial pneumonia associated with primary biliary cirrhosis (2008)
  • Author(s): Ishii H, Iwata A, et.al.
  • Journal Title: Respiratory medicine CME 1 Pages: 215-217
  • Peer Reviewed
• [Journal Article] High serum levels of thrombospondin-1 in patients with interstitial pneumonia
  • Author(s): Ide M, Ishii H, et. al.
  • Journal Title: Respiratory medicine (In press)
  • Peer Reviewed
• [Journal Article] Usual interstitial pneumonia associated with primary biliary cirrhosis
  • Author(s): Ishii H, Iwata A, et. al.
  • Journal Title: Respiratory medicine CME (In press)
  • Peer Reviewed
• [Presentation] 特発性間質性肺炎におけるthrombospondin-1の発現〜バイオマーカーとしてのTSP-1の検討〜 (2009)
  • Author(s): 石井寛
  • Organizer: 第5回呼吸器バイオマーカー研究会
  • Place of Presentation: 東京
  • Year and Date: 2009-03-07
• [Presentation] 特発性間質性肺炎におけるthrombospondin-1の発現 (2008)
  • Author(s): 石井寛
  • Organizer: 第49回日本呼吸器学会学術講演会
  • Place of Presentation: 神戸市
  • Year and Date: 2008-06-16
• [Presentation] Increased expression of thrombospondin-1 in patients with interstitial pneumonias (2007)
  • Author(s): Iwata A, Ishii H, et. al.
  • Organizer: Asian Pacific Society of Respirology
  • Place of Presentation: Gold coast, Australia
  • Year and Date: 2007-12-02