The mechanisms of protection against infection by pulmonary surfactant proteins

• Project/Area Number: 19790565
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Respiratory organ internal medicine
• Research Institution: Sapporo Medical University
• Principal Investigator: NISHITANI Chiaki Sapporo Medical University, 医学部, 助教 (30381255)
• Project Period (FY): 2007 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥3,690,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥390,000); Fiscal Year 2008: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2007: ¥2,000,000 (Direct Cost: ¥2,000,000)
• Keywords: 肺サーファクタント蛋白質 / トル様受容体(TLR) / 非定型抗酸菌 / レジオネラ / 自然免疫 / 細胞内寄生菌 / 呼吸器感染症 / リポ多糖(LPS) / コレクチン / リポアラビノマンナン

Research Abstract

本研究により、1)SP-Dがトル様受容体(TLR)介在炎症応答を制御すること、2)肺サーファクタント蛋白質がレジオネラなどの細胞内寄生菌と直接結合すること、さらに、肺サーファクタント蛋白質が、レジオネラの有するマクロファージへの膜障害作用を抑制すると共に、レジオネラの細胞内増殖を抑制することが明らかとなった。このことから、肺サーファクタント蛋白質がTLRを介する炎症制御ならびに細胞内寄生菌感染過程において重要な生体防御機能を担っていることが示された。

Research Products

• [Journal Article] Pulmonary surfactant protein D inhibits lipopolysaccharide (LPS)-induced inflammatory cell responses by altering LPS binding to its receptors (2008)
  • Author(s): Yamazoe M*, Nishitani C*, Takahashi M, Katoh T, Ariki S, Shimizu T, Mitsuzawa H, Sawada K, Voelker DR, Takahashi H, Kuroki Y.
  • Journal Title: J Biol Chem 283 (51) Pages: 35878-35888
  • Peer Reviewed
• [Journal Article] Pulmonary surfactant protein D binds MD-2 through the carbohydrate recognition domain (2008)
  • Author(s): Nie X*, Nishitani C*, Yamazoe M, Ariki S, Takahashi M, Shimizu T, Mitsuzawa H, Sawada K, Smith K, Crouch E, Nagae H, Takahashi H, Kuroki Y
  • Journal Title: Biochemistry 47 (48) Pages: 12878-12885
  • Peer Reviewed
• [Journal Article] A single base mutation in the PRAT4A gene reveals differential interaction of PRAT4A with Toll-like receptors (2008)
  • Author(s): Kiyokawa T, Akashi-Takamura S, Shibata T, Matsumoto F, Nishitani C, Kuroki Y, Seto Y, Miyake K
  • Journal Title: Int Immunol 20 (11) Pages: 1407-15
  • NAID: 10024357741
  • Peer Reviewed
• [Journal Article] Pulmonary surfactant protein D inhibits lipopolysaccharide (LPS)-induced inflammatory cell responses by altering LPS binding to its recentors (2008)
  • Author(s): Yamazoe M.
  • Journal Title: J. Biol. Chem 283 Pages: 35878-35888
  • Peer Reviewed
• [Journal Article] Pulmonary surfactant protein D binds MD-2 through the carbohydrate recognition domain (2008)
  • Author(s): Nie X.
  • Journal Title: Biochemistry 47 Pages: 12878-12885
  • Peer Reviewed
• [Journal Article] A single base mutation in the PRAT4A gene reveals differential inter action of PRAT4A with Toll-like receptors (2008)
  • Author(s): Kiyokawa T
  • Journal Title: Int. Immunol 20 Pages: 1407-1415
  • Peer Reviewed
• [Journal Article] The microtubule-binding protein Hook3 interacts with a cytoplasmic domain of scavenger receptor A (2007)
  • Author(s): Sano H, Ishino M, Kramer H, Shimizu T, Mitsuzawa H, Nishitani C, Kuroki Y
  • Journal Title: J Biol Chem 282 (11) Pages: 7973-7981
  • Peer Reviewed
• [Journal Article] Pulmonary collectins in innate immunity of the lung (2007)
  • Author(s): Kuroki Y, Takahashi M, Nishitani C
  • Journal Title: Cell Microbiol 9 (8) Pages: 1871-1879
  • Peer Reviewed
• [Journal Article] The microtubule-binding protein Hook3 interacts with a cytoplasmic domain of scavenger receptor A (2007)
  • Author(s): Sano H.
  • Journal Title: J Biol Chem 282 Pages: 7973-7981
  • Peer Reviewed
• [Journal Article] Pulmonary collectins in innate immunity of the lung (2007)
  • Author(s): Kuroki Y.
  • Journal Title: Cell Microbiol 9 Pages: 1871-1879
  • Peer Reviewed
• [Presentation] Pulmonary collectins exhibit anti-microbial activity against Legionella pneumophila (2008)
  • Author(s): 澤田格
  • Organizer: BMB2008 第81回日本生化学会大会
  • Place of Presentation: 神戸国際展示場
  • Year and Date: 2008-12-12
• [Presentation] Pulmonary collectins exhibit anti-microbial activity against Legionella pneumophila (2008)
  • Author(s): 澤田格
  • Organizer: BMB2008第81回日本生化学会大会
  • Place of Presentation: 神戸国際展示場
  • Year and Date: 2008-12-12
• [Presentation] Mutationalanalysis of Cys88 of Toll-Like receptor 4 highlights the critical role of MD-2 in cell surface receptor expression (2008)
  • Author(s): Chiaki Nishitani
  • Organizer: BMB2008 第81回日本生化学会大会
  • Place of Presentation: 神戸国際展示場
  • Year and Date: 2008-12-11
• [Presentation] Mutational analysis of Cys^<88> of Toll-Like receptor 4 highlights the critical role of MD-2 in cell surface receptor expression (2008)
  • Author(s): 西谷千明
  • Organizer: BMB2008第81回日本生化学会大会
  • Place of Presentation: 神戸国際展示場
  • Year and Date: 2008-12-11
• [Presentation] ナノ構造解析・センシングにおける小角散乱の利用高度化の将来展望 (2008)
  • Author(s): 松嶋範男
  • Organizer: TOLL様受容体の溶液構造PF研究会
  • Place of Presentation: 高エネルギー加速器研究機構・国際交流センター
  • Year and Date: 2008-09-18
• [Presentation] TOLL様受容体の溶液構造 (2008)
  • Author(s): 松嶋範男
  • Organizer: PF研究会「ナノ構造解析・センシングにおける小角散乱の利用高度化の将来展望」
  • Place of Presentation: 高エネルギー加速器研究機構・国際交流センター
  • Year and Date: 2008-09-18
• [Presentation] Pulmonary collectins interact with and aggregate Mycobacterium avium (2008)
  • Author(s): 有木茂
  • Organizer: The 8th Awaji International Forum on Infection and Immunity
  • Place of Presentation: 日兵庫県立淡路夢舞台国際会議場
  • Year and Date: 2008-09-10
• [Presentation] Pulmonary collectins interact with and aggregate Mycobacterium avium (2008)
  • Author(s): 有木茂
  • Organizer: The 8th Awaji International Forum on Infection and Immunity
  • Place of Presentation: 淡路夢舞台国際会議場
  • Year and Date: 2008-09-10
• [Presentation] TLR4の細胞表面発現におけるMD-2の重要性 (2008)
  • Author(s): 西谷千明
  • Organizer: JCS2008-Joint Conference in Sapporo 2008-
  • Place of Presentation: 北海道大学学術交流会館
  • Year and Date: 2008-07-11
• [Presentation] 肺サーファクタント蛋白質は非定型抗酸菌に結合し菌体凝集を惹起する (2008)
  • Author(s): 有木茂
  • Organizer: JCS2008-Joint Conference in Sapporo 2008-
  • Place of Presentation: 北海道大学学術交流会館
  • Year and Date: 2008-07-11
• [Presentation] レジオネラ菌に対する肺サーファクタント蛋白質の増殖抑制作用 (2008)
  • Author(s): 澤田格
  • Organizer: JCS2008-Joint Conference in Sapporo 2008-
  • Place of Presentation: 北海道大学学術交流会館
  • Year and Date: 2008-07-11
• [Presentation] TLR4の細胞表面発現におけるMD-2の重要性 (2008)
  • Author(s): 西谷千明
  • Organizer: JCS2008-Joint Conference in Sanporo 2008-
  • Place of Presentation: 北海道大学学術交流会館
  • Year and Date: 2008-07-11
• [Presentation] 肺コレクチンは非定型抗酸菌に結合し菌体凝集を惹起する (2008)
  • Author(s): 有木茂
  • Organizer: JCS2008-Joint Conference in Sapporo 2008-
  • Place of Presentation: 北海道大学学術交流会館
  • Year and Date: 2008-07-11
• [Presentation] レジオネラ菌に対する肺コレクチンの増殖抑制作用 (2008)
  • Author(s): 澤田格
  • Organizer: JCS2008-Joint Conference in Sapporo 2008-
  • Place of Presentation: 北海道大学学術交流会館
  • Year and Date: 2008-07-11
• [Presentation] Toll-like receptor 4 region Glu24-Lys47 is a site for MD-2 binding (2007)
  • Author(s): 西谷千明
  • Organizer: 第30回日本分子生物学会年会・第80回日本生化学会大会 合同大会
  • Place of Presentation: パシフィコ
  • Year and Date: 2007-12-13
• [Presentation] Toll-like receptor 4 region Glu24-Lys47 is a site for MD-2 binding (2007)
  • Author(s): 西谷 千明
  • Organizer: 第30回日本分子生物学会年会・第80回日本生化学会大会 合同大会
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 2007-12-13
• [Presentation] MD-2認識とリポ多糖シグナル伝達におけるToll様受容体4 N末端側Cys29及びCys40の重要性 (2007)
  • Author(s): 西谷千明
  • Organizer: 第20回内藤コンファレンス 自然免疫の医学・生物学[III]
  • Place of Presentation: 湘南国際村センター
  • Year and Date: 2007-10-10
• [Presentation] Surfactant protein A directly interacts with TLR4 and MD-2 and regulates inflammatory cellular response (2007)
  • Author(s): Chiaki Nishitani
  • Organizer: 第7回あわじしま感染症・免疫フォーラム
  • Place of Presentation: 兵庫県立淡路夢舞台国際会議場
  • Year and Date: 2007-09-03
• [Book] エンドトキシン研究10基礎と臨床の最新知見 (2007)
  • Author(s): 西谷千明,光澤博昭,清水健之,佐野仁美,松嶋範男,黒木由夫
  • Publisher: 医学図書出版株式会社
• [Book] エンドトキシン研究10基礎と臨床の最新知見 (2007)
  • Author(s): 西谷 千明
  • Total Pages: 7
  • Publisher: 医学図書出版株式会社