Role of CTGF in renal diseases in conditional knockout mice

• Project/Area Number: 19790581
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Kidney internal medicine
• Research Institution: Kyoto University
• Principal Investigator: YOKOI Hideki Kyoto University, 医学研究科・産学官連携, 助教 (90378779)
• Project Period (FY): 2007 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥3,630,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥330,000); Fiscal Year 2008: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2007: ¥2,200,000 (Direct Cost: ¥2,200,000)
• Keywords: 腎臓学 / CTGF / CreERT2 / コンディショナルノックアウトマウス / タモキシフェン / Cre リコンビナーゼ / 腎臓 / 糖尿病性腎症 / ポドサイト / CreER^<T2> / ネフリン / loxP / FRT / Rosa26Rマウス

Research Abstract

CTGFコンディショナルノックアウトマウスの作製を行った。Targetingコンストラクト、組み換えES細胞、キメラマウスを作製しhomozygous floxed CTGFマウスを確認した。Heterozygous floxed CTGFマウスと全身性の誘導性Creマウスとの交配により得られた変異マウスは、tamoxifenにより組み換えが起こることを確認した。さらにtamoxifenによる誘導型糸球体上皮細胞特異的CreERT2マウスを作製し、薬剤誘導性に上皮細胞特異的に組み換えが認められた。

Research Products

• [Journal Article] Urinary neutrophil gelatinase-associated lipocalin levels reflect damage in glomeruli, proximal and distal nephrons. (2009)
  • Author(s): Kuwabara T, Mori K, Mukoyama M, Kasahara M, Yokoi H, Saito Y, Yoshioka T, Ogawa Y, Imamaki H, Kusakabe T, Ebihara K, Omata M, Satoh N, Sugawara A, Barasch J, Nakao K.
  • Journal Title: Kidney Int 75 Pages: 285-294
  • Peer Reviewed
• [Journal Article] Urinary neutrophil gelatinase-associated lipocalin levels reflect damage to glomeruli, proximal tubules, and distal nephrons (2009)
  • Author(s): Kuwabara T, et.al.
  • Journal Title: Kidney Int. 75 Pages: 285-294
  • Peer Reviewed
• [Journal Article] Overexpression of connective tissue growth factor worsens diabetic nephropathy in mice. (2008)
  • Author(s): Yokoi H, Mukoyama M, Mori K, Kasahara M, Suganami T, Sawai K, Yoshioka T, Saito Y, Ogawa Y, Kuwabara T, Sugawara A, Nakao K.
  • Journal Title: Kidney Int 73 Pages: 446-455
  • Peer Reviewed
• [Journal Article] Adrenomedullin inhibits connective tissue growth factor expression, extracellular signal-regulated kinase activation and renal fibrosis. (2008)
  • Author(s): Nagae T, Mori K, Mukoyama M, Kasahara M, Yokoi H, Suganami T, Sawai K, Yoshioka T, Koshikawa M, Saito Y, Ogawa Y, Kuwabra T, Tanaka I, Sugawara A, Kuwahara T, Nakao K.
  • Journal Title: Kidney Int 74 Pages: 70-80
  • Peer Reviewed
• [Journal Article] Adrenomedullin inhibits connective tissue growth factor expression, extracellular signal-regulated kinase activation and renal fibrosis (2008)
  • Author(s): Nagae T, et.al.
  • Journal Title: Kidney Int. 74 Pages: 70-80
  • Peer Reviewed
• [Journal Article] Overexpression of connective tissue growth factor in podocytes worsens diabetic nephropathy in mice. (2008)
  • Author(s): Yokoi H.
  • Journal Title: Kidney Int. 73 Pages: 446-455
  • Peer Reviewed
• [Journal Article] Adrenomedullin inhibits connective tissue growth factor expression, extracellular signal-regulated kinase activation and renal fibrosis (2008)
  • Author(s): Nagae T.
  • Journal Title: Kidney Int. (in press)
  • Peer Reviewed
• [Journal Article] Expression of CCN1(CYR61) in developing, normal, and diseased human kidney. (2007)
  • Author(s): Sawai K, Mukoyama M, Mori K, Kasahara M, Koshikawa M, Yokoi H, Yoshioka T, Ogawa Y, Sugawara A, Nishiyama H, Yamada S, Kuwahara T. Saleem MA, Shiota K, Ogawa O, Miyazato M, Kangawa K, Nakao K.
  • Journal Title: Am J Physiol 293
  • Peer Reviewed
• [Journal Article] Expression of CCN1 (CYR61) in developing, normal, and diseased human kidney (2007)
  • Author(s): Sawai K.
  • Journal Title: Am J Physiol Renal Physiol 293 Pages: 1363-1372
  • Peer Reviewed
• [Presentation] ネフリンプロモーターによるタモキシフェン誘導性Cre過剰発現マウスの解析 (2008)
  • Author(s): 横井秀基ら
  • Organizer: 第51回日本腎臓学会学術総会
  • Place of Presentation: 福岡
  • Year and Date: 2008-05-30
• [Presentation] ネフリンプロモーターによるタモキシフェン誘導性Cre過剰発現マウスの解析 (2008)
  • Author(s): 横井秀基ら
  • Organizer: 第51回日本腎臓学会学術総会
  • Place of Presentation: 福岡市
  • Year and Date: 2008-05-30
• [Presentation] Overexpression of connective tissue growth factor (CTGF) in podocytes deteriorates diabetic nephropathy in mice (2007)
  • Author(s): Yokoi H et al.
  • Organizer: XLIV Congress of the European Renal Association
  • Place of Presentation: Barcelona, Spain.
  • Year and Date: 2007-06-24
• [Presentation] Overexpression of connective tissue growth factor (CTGF) in podocytes deteriorates diabetic nephropathy in mice (2007)
  • Author(s): Yokoi H.
  • Organizer: XLIV Congress of the European Renal Association
  • Place of Presentation: Barcelona, Spain
  • Year and Date: 2007-06-24
• [Presentation] 糸球体上皮細胞特異的CTGF過剰発現マウスを用いた糖尿病性腎症進展機序の検討 (2007)
  • Author(s): 横井秀基ら
  • Organizer: 第50回日本腎臓学会学術総会
  • Place of Presentation: 浜松
  • Year and Date: 2007-05-26
• [Presentation] 糖尿病性腎症進展におけるCTGFの意義 -糸球体上皮細胞特異的トランスジェニックマウスを用いた解析- (2007)
  • Author(s): 横井秀基ら
  • Organizer: 第50回日本糖尿病学会年次学術集会
  • Place of Presentation: 仙台
  • Year and Date: 2007-05-24
• [Remarks]
  • URL: http://www.kuhp.kyoto-u.ac.jp/~med2/index-jp.html
• [Remarks]
  • URL: http://www.kuho.kyoto-u.ac.jp/~med2/index-jp.html