Generation of phosphate sensor knock in mice

• Project/Area Number: 19790585
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Kidney internal medicine
• Research Institution: The University of Tokushima
• Principal Investigator: SEGAWA Hiroko The University of Tokushima, 大学院・ヘルスバイオサイエンス研究部, 助教 (70325257)
• Project Period (FY): 2007 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥3,780,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥480,000); Fiscal Year 2008: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2007: ¥1,700,000 (Direct Cost: ¥1,700,000)
• Keywords: 腎臓、 / リン / センサー / HHRH / 腎 / カルシウム / 骨 / 腎臓

Research Abstract

NPT2cの変異により引き起こされるクル病のメカニズムを明らかにすることを目的とし変異を有するノックインマウスの作製を行った。機能異常または、局在異常が認められた。最も症状の強い変異を用いてノックインマウス作製に入った。複数のラインを確認したが、変異リン・カルシウム代謝に大きな異常は見られていなかった。Npt2c変異はドミナントネガティブ作用を示さないことが示唆された。マウスにおいては他の分子が中心となっている可能性があることが、理由と考えられる。

Research Products

• [Journal Article] et al Type llc sodium-dependent phosphate transporter regulates calcium metabolism (2009)
  • Author(s): Segawa H
  • Journal Title: J Am Soc Nephrol 20 Pages: 104-113
  • Peer Reviewed
• [Journal Article] Type IIc sodium-dependent phosphate transporter regulates calcium metabolism (2009)
  • Author(s): Segawa H., et.al.
  • Journal Title: J Am Soc Nephrol. 20 Pages: 104-113
  • Peer Reviewed
• [Journal Article] Role of low protein and low phosphorus diet in the progression of chronic kidney disease in uremic rats. (2008)
  • Author(s): Kusano K. Segawa H. et al.
  • Journal Title: J Nutr Sci Vitaminol (Tokyo) 54 Pages: 237-243
  • NAID: 110006792025
  • Peer Reviewed
• [Journal Article] Role of low protein and low phosphorus diet in the progression of chronic kidney disease in uremic rats (2008)
  • Author(s): Kusano K., et.al.
  • Journal Title: J Nutr Sci Vitaminol (Tokyo). 54 Pages: 237-243
  • Peer Reviewed
• [Journal Article] Polypyrimidine tract-binding protein is involved in regulation of albumin synthesis in response to food intake (2008)
  • Author(s): Kuwahata M, et.al.
  • Journal Title: J Nutr Sci Vitaminol (Tokyo). 54 Pages: 142-147
  • Peer Reviewed
• [Journal Article] Management of phosphate in chronic kidney disease--Regulation of posphate homeostasis (2008)
  • Author(s): Segawa H., et.al.
  • Journal Title: Clinical Calcium 19 Pages: 159-165
• [Journal Article] Progress in the identification of intestinal Pi transport inhibitors (2008)
  • Author(s): Miyamoto K., et.al.
  • Journal Title: Clinical Calcium 19 Pages: 242-247
• [Journal Article] Control of phosphate appetite in young rats (2007)
  • Author(s): Ohnishi R, Segawa H. et al.
  • Journal Title: J Med Invest 54 Pages: 366-369
  • NAID: 130004822562
  • Peer Reviewed
• [Journal Article] New aspect of renal phosphate reabsorption:thetype IIc sodium-dependent phosphate transporter (2007)
  • Author(s): Miyamoto K, Ito M, Kuwahata M, Segawa H
  • Journal Title: Am J Nephrol. 27 Pages: 503-515
  • Peer Reviewed
• [Journal Article] Hereditary.hypophosphatemic rickets with hypercalciuria: a study for the phosphate transporter gene type IIc and osteoblastic function. (2007)
  • Author(s): Yamamoto T, Michigami T, Aranami F, SegawaH.etal.
  • Journal Title: J Bone Mineral Metab 25 Pages: 407-413
  • Peer Reviewed
• [Journal Article] Control of phosphate appetite in young rats. (2007)
  • Author(s): Ohnishi R, et. al.
  • Journal Title: J Med Invest 54 Pages: 366-369
  • Peer Reviewed
• [Journal Article] New aspect of renal phosphate reabsorption: the type IIc sodium-dependent phosphate transporter. (2007)
  • Author(s): Miyamoto K, et. al.
  • Journal Title: Am J Nephrol. 27 Pages: 503-515
  • Peer Reviewed
• [Journal Article] Hereditary hypophosphatemic rickets with hypercalciura: a study for the phosphate transporter gene type IIc and osteoblastic function. (2007)
  • Author(s): Yamamoto T, et. al.
  • Journal Title: J Bone Mineral Metab. 25 Pages: 407-413
  • Peer Reviewed
• [Presentation] Effect of Fibroblast Growth Factor (FGF)23 on Npt2a-/-, Npt2c-/- Double-Knockout (DKO) Mice. (2008)
  • Author(s): Tomoe Y, Segawa H. et al.
  • Organizer: The American Society of Nephrology Renal Week (2008)
  • Place of Presentation: San Francisco, CA, USA
  • Year and Date: 2008-11-08
• [Presentation] Effect of Fibroblast Growth Factor (FGF) 23 on Npt 2a-/-, Npt 2c-/-Double-Knockout (DKO) Mice (2008)
  • Author(s): Tomoe Y., et.al.
  • Organizer: The American Society of Nephrology Renal Week (2008)
  • Place of Presentation: San Francisco, CA, USA
  • Year and Date: 2008-11-08
• [Presentation] Mutation of Type Iic Na+-Dependent Phosphate Cotransporter (NaPi-IIc) and Hereditary Hypophosphatemic Ricket with Hypercalciuria (HHRH) (2008)
  • Author(s): Ito M. et al
  • Organizer: The American Society of Nephrology Renal week(2008)
  • Place of Presentation: San Francisco, CA, USA
  • Year and Date: 2008-11-07
• [Presentation] Mutation of Type IIc Na +-Dependent Phosphate Cotran sporter (NaPi-IIc) and Hereditary Hypophosphatemic Rickets with Hypercalciuria (HHRH) (2008)
  • Author(s): Ito M., et.al.
  • Organizer: The American Society of Nephrology Renal Week (2008)
  • Place of Presentation: San Francisco, CA, USA
  • Year and Date: 2008-11-07
• [Presentation] The Synergistic Role of Npt2a and Npt2c in Inorganic Phosphate Metabolism of Mice (2008)
  • Author(s): Segawa H. et al
  • Organizer: ASBMR30th Annual Meeting (2008)
  • Place of Presentation: Montreal, Quebec, Canada
• [Presentation] The Synergistic Role of Npt 2a and Npt 2c in Inorganic Phosphate Metabolism of Mice (2008)
  • Author(s): Segawa H., et.al.
  • Organizer: ASBMR 30th Annual Meeting (2008)
  • Place of Presentation: Montreal, Quebec, Canada
• [Presentation] Role of Ezrin in the Regulation of Membrane Localization of Type IIa Sodium-Dependent Phosphate Transporter (NaPi-IIa). (2007)
  • Author(s): Taketani Y. et al
  • Organizer: The American Society of Nephrology Renal Week (2007)
  • Place of Presentation: San Francisco, California, USA.
  • Year and Date: 2007-11-05
• [Presentation] Thyroid Hormones Regulate the Phosphate Homeostasis through the Transcriptional Control of the Renal Type IIa Sodium-Dependent Phosphate Cotransporter (NaPi-IIa) Gene. (2007)
  • Author(s): Yamamoto H. et al.
  • Organizer: The American Society of Nephrology Renal Week(2007)
  • Place of Presentation: San Francisco, California, USA.
  • Year and Date: 2007-11-05
• [Presentation] Role of Ezrin in the Regulation of Membrane LocaKzation of Type IIa Sodium-Dependent Phosphate Transporter (NaPi-IIa). (2007)
  • Author(s): Taketani Y., et. al.
  • Organizer: The American Society of Nephrology Renal Week(2007)
  • Place of Presentation: San Francisco, California,USA.
  • Year and Date: 2007-11-05
• [Presentation] Thyroid Hormones Regulate the Phosphate Homeostasis through the Transcriptional Control 0f the Renal Type IIa Sodium-Dependent Phosphate Cotransporter (NaPi-Ila) Gene. (2007)
  • Author(s): Yamamoto H., et. al.
  • Organizer: The American Society of Nephrology Renal Week(2007)
  • Place of Presentation: San Francisco, California,USA.
  • Year and Date: 2007-11-05
• [Presentation] Npt2a and Npt2c in Mice Play Distinct and Synergistic Rolesin Inorganic Phosphate Metabolism and Skeletal Development. (2007)
  • Author(s): Segawa H. et al.
  • Organizer: The American Society of Nephrology Renal Week (2007)
  • Place of Presentation: San Francisco, California, USA.
  • Year and Date: 2007-11-03
• [Presentation] The Molecular Mechanisms for the Alteration of PepT1 Expression in 3/4 Nephrectomized Rats (2007)
  • Author(s): Furutani J, Segawa H. et al
  • Organizer: The American Society of Nephrology Renal Week (2007)
  • Place of Presentation: San Francisco, California, USA.
  • Year and Date: 2007-11-03
• [Presentation] Npt2a and Npt2c in Mice Play Distinct and Synergistic Roles in Inorganic Phosphate Metabolism and Skeletal Development. (2007)
  • Author(s): Segawa H., et. al.
  • Organizer: The American Society of Nephrology Renal Week(2007)
  • Place of Presentation: San Francisco, California, USA.
  • Year and Date: 2007-11-03
• [Presentation] The Molecular Mechanisms for the Alteration of PepT1 Expression in 3/4 Nephrectomized Rats. (2007)
  • Author(s): Furutani J., et. al.
  • Organizer: The American Society of Nephrology Renal Week(2007)
  • Place of Presentation: San Francisco, California,USA.
  • Year and Date: 2007-11-03
• [Presentation] The Role of the Type IIc Sodium-dependent Phosphate Transporter (Npt2c), which Is Involved iHereditary Hypophosphatemic Rickets with Hypercalciuria (HHRH) (2007)
  • Author(s): Segawa H. et al
  • Organizer: ASBMR29th Annual Meeting(2007)
  • Place of Presentation: Honolulu, Hawaii, USA
  • Year and Date: 2007-09-17
• [Presentation] Sodium-dependent Phosphate Transporte(Npt2c), which Is Involved in Hereditary Hypophosphatemic Rickets with Hypercalciuria (HHRH) (2007)
  • Author(s): Tomoe Y, Segawa H. et al.
  • Organizer: ASBMR29th Annual Meeting (2007)
  • Place of Presentation: Honolulu, Hawaii, USA
  • Year and Date: 2007-09-17
• [Presentation] The Role of the Type IIc Sodium-dependent Phosphate Transporter (Npt2c), which Is Involved in Hereditary Hypophosphatemic Rickets with Hypercalciviria (HHRH) (2007)
  • Author(s): Segawa H., et. al.
  • Organizer: ASBMR29tli Annual Meeting (2007)
  • Place of Presentation: Honolulu, Hawaii, USA
  • Year and Date: 2007-09-17
• [Presentation] Sodium-dependent Phosphate Transporter (Npt2c), which Is Involved in Hereditary Hypophosphatemic Rickets with Hvpercalciuria (HHRH). (2007)
  • Author(s): Tomoe Y., et. al.
  • Organizer: ASBMR29th Annual Meeting (2007)
  • Place of Presentation: Honolulu, Hawaii, USA
  • Year and Date: 2007-09-16