The role of embryonic dorsal aorta Sca-1 positive cells in the renal endothelial regeneration

• Project/Area Number: 19790588
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Kidney internal medicine
• Research Institution: Jichi Medical University
• Principal Investigator: ITO Chiharu Jichi Medical University, 医学部, 助教 (00424010)
• Project Period (FY): 2007 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥3,160,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥360,000); Fiscal Year 2008: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2007: ¥1,600,000 (Direct Cost: ¥1,600,000)
• Keywords: 背側大動脈 / stem cell antigen-1 / 胎児背側大動脈 / VEGF / 低酸素 / TGF-β / Sca-1 / 内皮細胞

Research Abstract

マウス胎児背側大動脈由来Sca-1陽性細胞(EASCA1C)が,腎糸球体内皮修復に関与するかどうかの検討を行った。コラゲナーゼ処理背側大動脈初代培養後,胎児大動脈由来Sca-1陽性細胞を分離抽出した。EASCA1Cの内皮細胞への分化条件を,in vitroで検討したが,内皮細胞への分化は観察できなかった。
なお,マウス摘出背側大動脈3次元培養下の萌芽血管新生における,TGF-βの役割についても検討した。低酸素下,VEGF添加にて,コラーゲンゲル3次元培養下での管腔形成が促進し,TGF-βで阻害されることがわかった。

Research Products

• [Journal Article] TGF-β inhibits vascular sprouting through TGF-β type I receptor in the mouse embryonic aorta. (2009)
  • Author(s): Chiharu Ito, Tetsu Akimoto, Takashi Ioka, Takahisa Kobayashi, Eiji Kusano.
  • Journal Title: Tohoku J Exp Med. 218(1) Pages: 63-71
  • NAID: 10025106059
  • Peer Reviewed
• [Journal Article] Transactivation of RON receptor tyrosine kinase via PDGF receptor during steady-state growth of human mesangial cells. (2009)
  • Author(s): Takahisa Kobayashi1, Yusuke Furukawa, Jiro Kikuchi, Chiharu Ito, Yukio Miyata, Shigeaki, Muto, Akira Tanaka and Eiji Kusano.
  • Journal Title: Kidney Int 75(11) Pages: 1173-1183
  • Peer Reviewed
• [Journal Article] TGF-b inhibits vascular sprouting through TGF-btype I receptor in the mouse embryonic aorta (2009)
  • Author(s): Chiharu Ito, Tbtsu Akimoto, Takashi Ioka, Takahisa Kobayashi, Eiji Kusang
  • Journal Title: Tohoku J Exp Med 218(In press)
  • Peer Reviewed
• [Journal Article] Transactivation of RON receptor tyrosine kinase via PDGF receptor during steady-state growth of human mesangial cells
  • Author(s): Takahisa Kobayashi, Yusuke Furukawa, Jiro Kikuchi, Chiharu Ito, Yukio Miyata, Shigeaki Muto, Akira Tanaka, Eiji Kusano
  • Journal Title: Kidney Int (In press)
  • Peer Reviewed
• [Presentation] メサンギウム増殖性腎炎における受容体型チロシンキナーゼRONの発現 (2008)
  • Author(s): 小林高久, 古川雄祐, 菊池次郎, 伊藤千春, 武藤重明, 草野英二
  • Organizer: 第51回日本腎臓学会総会
  • Place of Presentation: 福岡国際会議場
  • Year and Date: 2008-05-30
• [Presentation] メサンギウム増殖性腎炎における受容体型チロシンキナーゼRONの発現 (2008)
  • Author(s): 小林高久,古川雄,菊池次郎,伊藤千春,武藤重明,草野英二
  • Organizer: 日本腎臓学会誌50巻3号Page340
• [Presentation] Role of transforming growth factor-beta in embryonic angiogenesis (2007)
  • Author(s): Chiharu Ito, Tetsu Akimoto, Takashi Ioka, Takahisa Kobayashi, Eiji Kusano
  • Organizer: American Society of Nephrology 40th
  • Place of Presentation: San Francisco
  • Year and Date: 2007-11-04
• [Presentation] Role of transforming growthfactor-beta in embryonic angiogenesis (2007)
  • Author(s): Chiharu Ito, Tetsu Akimoto, Takashi Ioka, Takahisa Kobayashi, Eiji Kusano
  • Organizer: Am Soc Nephrol
• [Presentation] Cross Talk between the Receptor Tyrosine Kinase RON and PDGF Receptor during Mesangial Cell Proliferation. (2007)
  • Author(s): Takahisa Kobayashi,Yusuke, Furukawa, Jiro Kikuchi, Chiharu Ito, Yukio Miyata, Shigeaki Muto, Eiji Kusano
  • Organizer: Am Soc Nephrol
• [Presentation] Erythropoietin (EPO)-Related cGMP Production Inversely Correlated with the Ratio of EPO Receptor (EPOR) Subtype mRNA in Circulatingndothelial Progenitor Cells(EPCs) of HD Patients and HUVECs (2007)
  • Author(s): Takashi Ioka, Shuichi Tsuruoka, Chiharu Ito, Hisashi Yamamoto, Tetsu Akimoto, Hideki Iwaguro, Takayuki Asahara, Akio Fujimura, Eiji Kusano
  • Organizer: Am Soc Nephrol
• [Presentation] 発生段階における血管形成へのTGF-βの影響 (2007)
  • Author(s): 伊藤千春,秋元哲,井岡崇,小林高久,草野英二
  • Organizer: 日本腎臓学会誌49巻3号Page268
• [Presentation] メサンギウム細胞増殖における受容体型チロシンキナーゼRONの役割 (2007)
  • Author(s): 小林高久,古川雄祐,菊池次郎,伊藤千春,宮田幸雄,武藤重明,草野英二
  • Organizer: 日本腎臓学会誌49巻3号Page307
• [Presentation] エリスロポエチン(EPO)誘発性血圧上昇は末梢血血管内皮前駆細胞における受容体(EPOR)サブタイプ比と相関する (2007)
  • Author(s): 井岡崇,鶴岡秀一,伊藤千春,秋元哲,山本尚史,岩畔英樹,浅原孝之,藤村昭夫,草野英二
  • Organizer: 日本腎臓学会誌49巻3号Page260