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Search for microRNA capable of modifying with ADAR2 activity

Research Project

Project/Area Number 19790601
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeSingle-year Grants
Research Field Neurology
Research InstitutionThe University of Tokyo

Principal Investigator

YAMASHITA Takenari  The University of Tokyo, 医学部附属病院, リサーチ・フェロー (20431843)

Project Period (FY) 2007 – 2008
Project Status Completed (Fiscal Year 2008)
Budget Amount *help
¥2,570,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥270,000)
Fiscal Year 2008: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2007: ¥1,400,000 (Direct Cost: ¥1,400,000)
KeywordsADAR2 / RNA編集 / 筋萎縮性側索硬化症 / microRNA / AMPA 受容体 / AMPA受容体
Research Abstract

孤発性ALSの運動ニューロン死に深く関わるRNA編集酵素ADAR2活性をin vitroで評価するシステムを開発した。その系を用いADAR2活性に影響を与えた3種のmicroRNAsを同定した。1種はADAR2活性を賦活したが、2種のmicroRNAsはADAR2活性を抑える作用があった。後者2種のmicroRNAsはADAR2 mRNA対GluR2ミニ遺伝子転写物比(酵素対基質量比)依存的にADAR2活性を抑制したが、ADAR2活性賦活の分子メカニズムは明らかにできなかった。microRNAsはADAR2活性に効果を与えることが明らかとなり、ADAR2活性の賦活による孤発性ALSの治療戦略のツールとして、有用であることが期待される。

Report

(3 results)
  • 2008 Annual Research Report   Final Research Report ( PDF )
  • 2007 Annual Research Report
  • Research Products

    (36 results)

All 2009 2008 2007 Other

All Journal Article (7 results) (of which Peer Reviewed: 6 results) Presentation (25 results) Book (4 results)

  • [Journal Article] Effects of antidepressants on GluR2 Q/R site-RNA editing in modified HeLa cell line.2009

    • Author(s)
      Sawada J, Yamashita T, Aizawa H, Aburakawa Y, Hasebe N, Kwak S
    • Journal Title

      Neurosci. Res. in press

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Newly identified ADAR2-mediated editing positions as a useful tool for ALS research.2008

    • Author(s)
      Kwak S, Nishimoto Y, Yamashita T
    • Journal Title

      RNA Biology 5

      Pages: 193-197

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Determination of editors at the novel A-to-I editing positions2008

    • Author(s)
      Nishimoto Y, Yamashita T, Hideyama T, Tsuji S, Suzuki N, Kwak S
    • Journal Title

      Neurosci. Res. 61(2)

      Pages: 201-206

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Determination of editors at the novel A-to-I editing positions2008

    • Author(s)
      Nishimoto Y., Yamashita T., Hideyama T., Tsuji S., Suzuki N., Kwak S.
    • Journal Title

      Neuroscience Research 61

      Pages: 201-206

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Newly identified ADAR2-mediated editing positions as a useful tool for ALS research2008

    • Author(s)
      Kwak S., Nishimoto Y., Yamashita T.
    • Journal Title

      RNA Biology 5

      Pages: 193-197

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Determination of editors at the novel A-to-I editing positions2008

    • Author(s)
      Nishimoto Y., Yamashita T., Hideyama T., Tsuji S. Suzuki N., Kwak S.
    • Journal Title

      Neuroscience Research (掲載確定)

    • Related Report
      2007 Annual Research Report
  • [Journal Article] Effects of antidepressants on GluR2 Q/R site-RNA editing in modified HeLa cellline

    • Author(s)
      Sawada J., Yamashita T., Aizawa H., Aburakawa Y., Hasebe N., Kwak S.
    • Journal Title

      Neuroscience Research (印刷中)

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Presentation] Regulation mechanism of GluR2 Q/R site-editing in cultured cell lines2008

    • Author(s)
      Yamashita T, Tadami C, Nishimoto Y, Hideyama T, Kimura D, Suzuki T, Kwak S
    • Organizer
      Society for neuroscience 38th Annual Meeting
    • Place of Presentation
      Washington, DC
    • Year and Date
      2008-11-17
    • Related Report
      2008 Final Research Report
  • [Presentation] Slow neuronal death of motor neurons in sporadic ALS mouse model by RNA editing enzyme ADAR2 knockout.2008

    • Author(s)
      Hideyama T, Yamashita T, Tsuji S, Takahashi R, Misawa H, Suzuki T, Kwak S
    • Organizer
      38th Annual Meeting Society for Neuroscience
    • Place of Presentation
      Washington
    • Related Report
      2008 Final Research Report
  • [Presentation] Slow death of motor neurons in sporadic ALS mouse model by conditional targeting of RNA editing enzyme ADAR22008

    • Author(s)
      Hideyama T, Yamashita T, Tsuji S, Takahashi R, Misawa H, Suzuki T, Kwak S
    • Organizer
      The 19th International Symposium on MND/ALS. 2008
    • Place of Presentation
      Birmingham
    • Related Report
      2008 Final Research Report
  • [Presentation] Sporadic ALS model mice by RNA editing enzyme abnormality.2008

    • Author(s)
      Hideyama T, Yamashita T, Tsuji S, Takahashi R, Misawa H, Suzuki T, Kwak S
    • Organizer
      6th Forum of European Neuroscience Forum 2008
    • Place of Presentation
      Geneve
    • Related Report
      2008 Final Research Report
  • [Presentation] 培養細胞によるGluR2 Q/R部位の編集制御機構2008

    • Author(s)
      山下雄也, 只見智恵子, 西本祥仁, 日出山拓人, 木村大輔, 鈴木岳之, 郭伸
    • Organizer
      第31回神経科学大会 Neuro2008
    • Place of Presentation
      東京
    • Related Report
      2008 Final Research Report
  • [Presentation] RNA編集異常による孤発性筋萎縮性側索硬化症モデルマウスの作製2008

    • Author(s)
      郭 伸, 日出山拓人, 山下雄也, 辻省次, 高橋良輔, 三澤日出巳, 木村大輔, 鈴木岳之
    • Organizer
      第31回神経科学大会、Neuro2008
    • Place of Presentation
      東京
    • Related Report
      2008 Annual Research Report 2008 Final Research Report
  • [Presentation] AMPA受容体サブユニットGluR2のQ/R部位RNA編集率に及ぼす各種薬剤の効果2008

    • Author(s)
      澤田 潤, 相澤仁志, 山下雄也, 油川陽子, 長谷部直幸, 郭 伸
    • Organizer
      第49回日本神経学会総会
    • Place of Presentation
      横浜
    • Related Report
      2008 Annual Research Report 2008 Final Research Report
  • [Presentation] RNA編集異常による孤発性ALSモデルマウスの開発2008

    • Author(s)
      日出山拓人, 西本祥仁, 山下雄也, 辻省次, 高橋良輔, 三澤日出巳, 鈴木岳之, 郭 伸
    • Organizer
      第49回日本神経学会総会
    • Place of Presentation
      横浜
    • Related Report
      2008 Annual Research Report 2008 Final Research Report
  • [Presentation] Sporadic ALS model mice by RNA editing enzyme abnormality2008

    • Author(s)
      Hideyama T, Yamashita T, Tsuji S, Takahashi R, Misawa H. Suzuki T. Kwak S
    • Organizer
      6^<th> Forum of European Neuroscience Forum 2008
    • Place of Presentation
      Geneve, スイス国
    • Related Report
      2008 Annual Research Report
  • [Presentation] 培養細胞によるGluR2Q/R部位の編集制御機構2008

    • Author(s)
      山下雄也, 只見智恵子, 西本祥仁, 日出山拓人, 木村大輔, 鈴木岳之, 郭伸
    • Organizer
      第31回神経科学大会、Neuro2008
    • Place of Presentation
      東京
    • Related Report
      2008 Annual Research Report
  • [Presentation] Slow death of motor neurons in sporadic ALS mouse model by conditi onal targeting of RNA editing enzyme ADAR22008

    • Author(s)
      Hideyama T, Yamashita T, Tsuji S, Takahashi R, Misawa H. Suzuki T, Kwak S
    • Organizer
      The 19^<th> International Symposium on MND/ALS
    • Place of Presentation
      Birmingham, 英国
    • Related Report
      2008 Annual Research Report
  • [Presentation] Regulation mechanism of G1uR2 Q/R site-editing in cultured cell lines2008

    • Author(s)
      Yamashita T, Tadami C, Nishimoto Y, Hideyama T, Kimura D, Suzuki T, Kwak S
    • Organizer
      Society for neuroscience 38th Annual Meeting
    • Place of Presentation
      Washington DC, 米国
    • Related Report
      2008 Annual Research Report
  • [Presentation] Slow neuronal death of motor neurons in sporadic ALS mouse model by RNA editing enzyme ADAR2 knockout2008

    • Author(s)
      Hideyama T, Yamashita T, Tsuji S, Takahashi R, Misawa H, Suzuki T, Kwak S
    • Organizer
      Society for neuroscience 38th Annual Meeting
    • Place of Presentation
      Washington DC, 米国
    • Related Report
      2008 Annual Research Report
  • [Presentation] Establishment of a novel Hela cell line stably expressing the half-echted GluR2 transcript2007

    • Author(s)
      Yamashita T., Tadami C., Nishimoto Y., Kimura D., Suzuki T., Kwak S.
    • Organizer
      Society for Neuroscience 37th Annual Meeting
    • Place of Presentation
      San Diego, California
    • Year and Date
      2007-11-06
    • Related Report
      2007 Annual Research Report
  • [Presentation] Tet onシステムを利用したAMPA受容体サブユニットGluR2のRNA編集率測定株の樹立2007

    • Author(s)
      山下雄也、只見知恵子、西本祥仁、木村大輔、鈴木岳之、郭伸
    • Organizer
      東京大学生命科学研究ネットワークシンポジウム2007
    • Place of Presentation
      東京
    • Year and Date
      2007-09-15
    • Related Report
      2007 Annual Research Report
  • [Presentation] Tet Onシステムを利用したRNA編集活性測定株の樹立2007

    • Author(s)
      山下雄也、只見知恵子、西本祥仁、木村大輔、鈴木岳之、郭伸
    • Organizer
      第50回日本神経化学会大会合同大会Neuro2007
    • Place of Presentation
      横浜
    • Year and Date
      2007-09-10
    • Related Report
      2007 Annual Research Report
  • [Presentation] Establishment of a novel Hela cell line stably expressing the half-edited GluR2 transcript.2007

    • Author(s)
      Yamashita, T, Tadami, C, Nishimoto,Y, Kimura, D, Suzuki,T, Kwak, S
    • Organizer
      Society for Neuroscience 37th Annual Meeting, No. 591.16.
    • Place of Presentation
      San Diego, California
    • Related Report
      2008 Final Research Report
  • [Presentation] Death of motor neurons in mice deficient in an RNA editing enzyme2007

    • Author(s)
      Hideyama, T, Yamashita, T, Tsuji, S, Takahashi, R, Misawa, H, Suzuki, T, Kwak, S
    • Organizer
      Amiotroph Later Scl, P153
    • Place of Presentation
      Tronto, Canada
    • Related Report
      2008 Final Research Report
  • [Presentation] Tet onシステムを利用したRNA編集活性測定株の樹立2007

    • Author(s)
      山下雄也, 只見知恵子, 西本祥仁, 木村大輔, 鈴木岳之, 郭伸
    • Organizer
      第50回日本神経化学会(横浜)大会合同大会 Neuro2007、P1-j01
    • Place of Presentation
      横浜
    • Related Report
      2008 Final Research Report
  • [Presentation] RNA編集異常による孤発性ALSモデルマウスの開発2007

    • Author(s)
      郭伸, 日出山拓人, 西本祥仁, 山下雄也, 辻省次, 高橋良輔, 三澤日出巳, 鈴木岳之
    • Organizer
      第30回日本神経科学会合同大会 Neuro2007、P1-h47
    • Place of Presentation
      横浜
    • Related Report
      2008 Final Research Report
  • [Presentation] Alteration of RNA editing and neuronal death in motor neuron diseases.2007

    • Author(s)
      Hideyama, T, Nishimoto, Y, Yamashita, T, Tsuji, S, Takahashi, H, Kakita, A, Suzuki, T, Kwak, S
    • Organizer
      ENS 2007, No. 700.
    • Place of Presentation
      Rhodes
    • Related Report
      2008 Final Research Report
  • [Presentation] 孤発性筋萎縮性側索硬化症のRNA編集異常2007

    • Author(s)
      日出山拓人, 西本祥仁, 山下雄也, 伊藤杏子, 辻省次, 高橋良輔, 三澤日出巳, 鈴木岳之, 郭伸
    • Organizer
      第48回日本神経学会総会、No. 10022
    • Place of Presentation
      名古屋
    • Related Report
      2008 Final Research Report
  • [Presentation] 孤発性ALSのバイオマーカーの開発2007

    • Author(s)
      西本祥仁, 日出山拓人, 山下雄也, 鈴木則宏, 郭伸
    • Organizer
      第48回日本神経学会総会、P-10122
    • Place of Presentation
      名古屋
    • Related Report
      2008 Final Research Report
  • [Presentation] グリオーマ細胞におけるAMPA受容体サブユニットGluR2のRNA編集率の検討2007

    • Author(s)
      只見智恵子, 木村大輔, 伊藤杏子, 山下雄也, 鈴木岳之, 郭伸
    • Organizer
      第80回日本薬理学会年会、No. 10300
    • Place of Presentation
      名古屋
    • Related Report
      2008 Final Research Report
  • [Presentation] 孤発性ALSのバイオマーカーの開発2007

    • Author(s)
      西本祥仁、日出山拓人、山下雄也、鈴木則宏、郭 伸
    • Organizer
      第48回日本神経学会総会
    • Place of Presentation
      名古屋
    • Related Report
      2007 Annual Research Report
  • [Book] AMPA receptor-mediated neuronal death in motor neuron diseases. In: Amino Acid Receptor Research, Eds. Paley BF, Warfield TE2008

    • Author(s)
      Kwak S, Hideyama T, Yamashita T
    • Publisher
      Nova Science Publishers Inc. NY.
    • Related Report
      2008 Final Research Report
  • [Book] In Amino Acid Receptor Research (AMPA receptor-mediated neuronal death in motor neuron diseases)2008

    • Author(s)
      Kwak S., Hideyama T., Yamashita T
    • Publisher
      Nova Science Publishers Inc. NY
    • Related Report
      2008 Annual Research Report 2007 Annual Research Report
  • [Book] グルタミン酸受容体と神経細胞死、神経変性疾患のサイエンス2007

    • Author(s)
      山下雄也, 郭 伸
    • Publisher
      南山堂
    • Related Report
      2008 Final Research Report
  • [Book] 神経変性疾患のサイエンス(グルタミン酸受容体と神経細胞死)2007

    • Author(s)
      山下 雄也, 郭 伸
    • Publisher
      南山堂
    • Related Report
      2007 Annual Research Report

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Published: 2007-04-01   Modified: 2016-04-21  

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