Revealing the role of Notch signaling in pancreatic development and its application for regenerative medicine

• Project/Area Number: 19790646
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Endocrinology
• Research Institution: Kyoto University
• Principal Investigator: FUJIKURA Junji Kyoto University, 大学院医学研究科, 助教 (70378743)
• Project Period (FY): 2007 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥3,780,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥480,000); Fiscal Year 2008: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2007: ¥1,700,000 (Direct Cost: ¥1,700,000)
• Keywords: 膵臓 / Notchシグナル / Rbp-j・Ptf1a (p48) / 糖尿病 / ノックアウトマウス / Rbp-j / Ptf1a(p48) / Ptfla(p48)

Research Abstract

膵発生の特に初期において、Notch/Rbp-jシグナルがPdx1発現を維持しながら膵幹細胞プールを維持していることを明らかとした。シグナルのリガンドとしてはNotch2の意義は乏しいことや、膵発生・再生関連転写因子とNotch/Rbp-j/Hes1シグナルとの間の相互作用の存在も明らかとなった。

Research Products

• [Journal Article] Pdx-1-independent differentiation of mouse embryonic stem cells into insulin-expressing cells. (2008)
  • Author(s): Takayama I, Miyazaki S, Tashiro F, Fujikura J, Miyazaki J, Yamato E.
  • Journal Title: Diabetes Res Clin Pract. Feb;79(2)
  • Peer Reviewed
• [Journal Article] Pdx-1-independent differentiation of mouse embryonic stem cells into insulin-expressing cells (2008)
  • Author(s): Takayama I, Miyazaki S, Tashiro F, Fujikura J, Miyazaki J, Yamato E
  • Journal Title: Diabetes Research and Clinical Practice 79
  • Peer Reviewed
• [Journal Article] Pdx-1-independent differentiation of mouse embryonic stem cells into insulin-expressing cells. (2008)
  • Author(s): Takayama I, Miyazaki S, Tashiro F, Fujikura J, Miyazaki J, Yamato E.
  • Journal Title: Diabetes Research and Clinical Practice 148 Pages: 8-10
  • Peer Reviewed
• [Journal Article] Rbp-j regulates expansion of pancreatic epithelial cells and their differentiation into exocrine cells during mouse development. (2007)
  • Author(s): Fujikura J, Hosoda K, Kawaguchi Y, Noguchi M, Iwakura H, Odori S, Mori E, Tomita T, Hirata M, Ebihara K, Masuzaki H, Fukuda A, Furuyama K, Tanigaki K, Yabe D, Nakao K.
  • Journal Title: Dev Dyn. Oct;236(10) Pages: 2779-2791
  • Peer Reviewed
• [Journal Article] Ceramide and adenosine 5'-monophosphate-activated protein kinase are two novel regulators of 11beta-hydroxysteroid dehydrogenase type 1 expression and activity in cultured preadipocytes. (2007)
  • Author(s): Arai N, Masuzaki H, Tanaka T, Ishii T, Yasue S, Kobayashi N, Tomita T, Noguchi M, Kusakabe T, Fujikura J, Ebihara K, Hirata M, Hosoda K, Hayashi T, Sawai H, Minokoshi Y, Nakao K.
  • Journal Title: Endocrinology Nov;148(11) Pages: 5268-77
  • Peer Reviewed
• [Journal Article] Genetic and pharmacological inhibition of Rho-associated kinase II enhances adipogenesis. (2007)
  • Author(s): Noguchi M, Hosoda K, Fujikura J, Fujimoto M, Iwakura H, Tomita T, Ishii T, Arai N, Hirata M, Ebihara K, Masuzaki H, Itoh H, Narumiya S, Nakao K.
  • Journal Title: J Biol Chem. 5;282(40) Pages: 29574-83
  • Peer Reviewed
• [Journal Article] Augmentation of 11beta-hydroxysteroid dehydrogenase type 1 in LPS-activated J774.1 macrophages--role of 11beta-HSD1 in pro-inflammatory properties in macrophages. (2007)
  • Author(s): Ishii T, Masuzaki H, Tanaka T, Arai N, Yasue S, Kobayashi N, Tomita T, Noguchi M, Fujikura J, Ebihara K, Hosoda K, Nakao K.
  • Journal Title: FEBS Lett. Feb 6;581(3) Pages: 349-54
  • Peer Reviewed
• [Journal Article] A case of secretin-responsive insulinoma with low serum C-peptide levels. (2007)
  • Author(s): Fujikura J, Hosoda K, Noguchi M, Ebihara K, Masuzaki H, Hirata M, Fujimoto K, Doi R, Iwanishi M, Nakao K.
  • Journal Title: Endocr J. Feb;54(1) Pages: 113-21
  • NAID: 10019479541
  • Peer Reviewed
• [Journal Article] Rbp-j regulates expansion of pancreatic epithelial cells and their differentiation into exocrine cells during mouse development. (2007)
  • Author(s): Fujikura J, Hosoda K, Kawaguchi Y, Noguchi M, Iwakura H, Odori S, Mori E, Tomita T, Hirata M, Ebihara K, Masuzaki H, Fukuda A, Furuyama K, Tanigaki K, Yabe D, Nakao K.
  • Journal Title: Developmental Dynamics 236 Pages: 2779-2791
  • Peer Reviewed
• [Journal Article] Genetic and pharmacological inhibition of Rho-associated kinase II enhances adipogenesis. (2007)
  • Author(s): Noguchi M, Hosoda K., Fujikura J, Fujimoto M, Iwakura H, Tomita T, Ishii T, Arai N, Hirata M, Ebihara K, Masuzaki H, Itoh H, Narumiya S, Nakao K.
  • Journal Title: The Journal of Biological Chemistry 282 Pages: 29574-29583
  • Peer Reviewed
• [Presentation] (2008)
  • Author(s): 藤倉純二、細田公則、川口義弥、野口倫生、岩倉浩、冨田努、小鳥真司、森栄作、泰江慎太郎、石井崇子、岡田定規、日下部徹、平田雅一、海老原健、益崎裕章、谷垣健二、矢部大介、中尾一和
  • Organizer: 第51回日本糖尿病学会年次学術集会
  • Place of Presentation: 東京国際フォーラム
  • Year and Date: 2008-05-24
• [Presentation] 膵特異的Rbp-jノックアウトマウスの検討 (2008)
  • Author(s): 藤倉純二、細田公則、川口義弥、野口倫生、岩倉浩、冨田努、小鳥真司、森栄作、泰江慎太郎、石井崇子、岡田定規、目下部徹、平田雅一、海老原健、益崎裕章、谷垣健二、矢部大介、中尾一和
  • Organizer: 第51回日本糖尿病学会年次学術集会
  • Place of Presentation: 東京国際フォーラム
  • Year and Date: 2008-05-24
• [Presentation] 膵特異的Rbp-jノックアウトマウスの検討 (2008)
  • Author(s): 藤倉純二、細田公則、川口義弥、野口倫生、岩倉浩、冨田努、小鳥真司、森栄作、泰江慎太郎、石井崇子、岡田定規、日下部徹、平田雅一、海老原健、益崎裕章、谷垣健二、矢部大介、中尾一和
  • Organizer: 第81回日本内分泌学会学術集会
  • Place of Presentation: 青森市文化会館
  • Year and Date: 2008-05-18
• [Presentation] 膵特異的Rbp-jノックアウトマウスの検討 (2007)
  • Author(s): 藤倉 純二、野口 倫生、冨田 努、岩倉 浩、細田 公則、川口 義弥、中尾 一和
  • Organizer: 第80回日本内分泌学会総会
  • Place of Presentation: 東京国際フォーラム
  • Year and Date: 2007-06-16
• [Presentation] 膵特異的Rbp-j ノックアウトマウスの検討 (2007)
  • Author(s): 藤倉 純二、野口 倫生、冨田 努、岩倉浩、細田 公則、川口 義弥、中尾 一和
  • Organizer: 第80回 日本内分泌学会総会
  • Place of Presentation: 東京国際フォーラム
  • Year and Date: 2007-06-16
• [Presentation] 膵特異的Rbp-jノックアウトマウスの検討 (2007)
  • Author(s): 藤倉 純二、野口 倫生、冨田 努、岩倉 浩、細田 公則、川口 義弥、中尾 一和
  • Organizer: 第50回日本糖尿病学会年次学術集会
  • Place of Presentation: 東京国際フォーラム
  • Year and Date: 2007-05-24
• [Presentation] 膵特異的Rbp-j ノックアウトマウスの検討 (2007)
  • Author(s): 藤倉 純二、野口 倫生、冨田 努、岩倉浩、細田 公則、川口 義弥、中尾 一和
  • Organizer: 第50回 日本糖尿病学会年次学術集会
  • Place of Presentation: 東京国際フォーラム
  • Year and Date: 2007-05-24
• [Remarks]
  • URL: http://www.kuhp.kyoto-u.ac.jp/~med2/jpn/research/diabetes.html
• [Remarks]
  • URL: http://www.kuhp.kyoto-u.ac.jp/~med2/jpn/research/diabetes.html