Development of a drug-indueible ghrelinoma model mouse to elucidate the chronic effects of ghrelin and to establish a ghrelmoma cell line

• Project/Area Number: 19790647
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Endocrinology
• Research Institution: Kyoto University
• Principal Investigator: IWAKURA Hiroshi Kyoto University, 大学院・医学研究科, 助教 (20378615)
• Project Period (FY): 2007 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥3,690,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥390,000); Fiscal Year 2008: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2007: ¥2,000,000 (Direct Cost: ¥2,000,000)
• Keywords: グレリン / トランスジェニックマウス / 腫瘍

Research Abstract

今回、作製を試みた薬剤誘導性のグレリン細胞腫瘍化マウスは、十分な発現レベルを得られず、グレリン細胞の腫瘍化を確認できなかった。しかし、従来から作製を試みてきた薬剤誘導性ではない、グレリン細胞腫瘍化マウスの作製に成功した。このマウスでは、最大で対照マウスの10倍程度の血中グレリン濃度の上昇を認めた。それに伴い、血清IGF-I値の上昇が認められ、血中グレリンの慢性的な上昇によりGH-IGF-I軸が刺激されたことが示唆された。今後、さらに細胞株の樹立を目指す。

Research Products

• [Journal Article] for the OA-THR Clinical Study Team Effects of ghrelin treatment in patients undergoing total hip replacement for osteoarthritis: Different outcomes from studies in patients with cardiac and pulmonary cachexia (2008)
  • Author(s): Akamizu T, Iwakura H, Ariyasu H, Murayama T, Sumi E, Teramukai S, Goto K, Ohnishi E, Akiyama H, Kawanabe K, Nankaku M, Ichihashi N, Tsuboyama T, Tamai K, Kataoka M, Nakamura T, Kangawa K
  • Journal Title: J Am Geriatr Soc 56(12) Pages: 2363-5
  • Peer Reviewed
• [Journal Article] Establishment of a novel neuroblastoma mouse model (2008)
  • Author(s): Iwakura H, Ariyasu H, Kanamoto N, Hosoda K, Nakao K, Kangawa K, Akamizu T.
  • Journal Title: Int J Oncol 33(6) Pages: 1195-9
  • Peer Reviewed
• [Journal Article] Efficacy of Ghrelin as a therapeutic approach for age-related physiologic changes (2008)
  • Author(s): Ariyasu H, Iwakura H, Yamada G, Nakao K, Kangawa K, Akamizu T
  • Journal Title: Endocrinology 149(7) Pages: 3722-8
  • Peer Reviewed
• [Journal Article] Repeated administration of ghrelin to patients with functional dyspepsia: its effects on food intake and appetite (2008)
  • Author(s): Akamizu T, Iwakura H, Ariyasu H, Hosoda H, Murayama T, Yokode M, Teramukai S, Seno H, Chiba T, Noma S, Nakai Y, Fukunaga M, Nakai Y,Kangawa K, FD Clinical Study Team
  • Journal Title: Eur J Endocrinol. 158(4) Pages: 491-8
  • Peer Reviewed
• [Journal Article] Establishment of a novel neuroblastoma mouse model (2008)
  • Author(s): 岩倉浩
  • Journal Title: Int J Oncol 33 Pages: 1195-9
  • Peer Reviewed
• [Journal Article] Effects of ghrelin treatment in patients undergoing total hip replacement for osteoarthritis : Different outcomes from studies in patients with cardiac and 〓 (2008)
  • Author(s): 赤水尚史
  • Journal Title: J Am Geriatr Soc 56 Pages: 2363-6
  • Peer Reviewed
• [Journal Article] Efficacy of Ghrelin as a therapeutic approach for age-related uhvsiologic changes (2008)
  • Author(s): 有安宏之
  • Journal Title: Endocrinology 149 Pages: 3722-8
  • Peer Reviewed
• [Journal Article] Repeated administration of ghrelin to patients with functional dvsnepsia : its effects on food intake and appetite (2008)
  • Author(s): 赤水尚史
  • Journal Title: Eur J Endocrinol 158 Pages: 491-8
  • Peer Reviewed
• [Journal Article] Rbp-j regulates expansion of pancreatic epithelial cells and their differentiation into exocrine cells during mouse development (2007)
  • Author(s): Fujikura J, Hosoda K, Kawaguchi Y, Noguchi M, Iwakura H, Odori S, Mori E, Tomita T, Hirata M, Ebihara K, Masuzaki H, Fukuda A, Furuyama K, Tanigaki K, Yabe D, Nakao K
  • Journal Title: Dev Dyn. 236(10) Pages: 2779-91
  • Peer Reviewed
• [Journal Article] Genetic and pharmacological inhibition of Rho-associated kinase II enhances adipogenesis (2007)
  • Author(s): Noguchi M, Hosoda K, Fujikura J, Fujimoto M, Iwakura H, Tomita T, Ishii T, Arai N, Hirata M, Ebihara K, Masuzaki H, Itoh H, Narumiya S, Nakao K
  • Journal Title: J Biol Chem. 282(40) Pages: 29574-83
  • Peer Reviewed
• [Journal Article] Effects of ghrelin administration on decreased growth hormone status in obese animals (2007)
  • Author(s): Iwakura H, Akamizu T, Ariyasu H, Irako T, Hosoda K, Nakao K, Kangawa K
  • Journal Title: Am J Physiol Endocrinol Metab. 293(3)
  • Peer Reviewed
• [Journal Article] Effects of ghrelin administration on decreased growth ho rmone status in obese animals (2007)
  • Author(s): 岩倉 浩
  • Journal Title: American Journal of Physiology Endocrinology and Metabolism 293 Pages: 819-25
  • Peer Reviewed
• [Journal Article] Genetic and pharmacological inhibition of Rho-associatedkinase II enhances adipogenesis (2007)
  • Author(s): 野口倫生
  • Journal Title: Journal of Biological Chemistry 282 Pages: 29574-83
  • Peer Reviewed
• [Journal Article] Rbp-j regulates expansion of pancreatic epithelial cells and their differentiation into exocrine cells during mouse development (2007)
  • Author(s): 藤倉純二
  • Journal Title: Developmental Dynamics 236 Pages: 2779-91
  • Peer Reviewed
• [Presentation] Effects of ghrelin administration on glucose metabolism and beta cell mass in rats with partial pancreatectomy (2008)
  • Author(s): Iwakura H
  • Organizer: The endocrine society's 90th annual meeting
  • Place of Presentation: サンフランシスコ
  • Year and Date: 2008-07-17
• [Presentation] ラット膵部分切除モデルに対するグレリン投与による効果の検討 (2008)
  • Author(s): 岩倉浩
  • Organizer: 第51回糖尿病学会総会
  • Place of Presentation: 東京
  • Year and Date: 2008-05-23
• [Presentation] ラット膵部分切除モデルに対するグレリン投与による効果の検討 (2008)
  • Author(s): 岩倉浩
  • Organizer: 第51回日本糖尿病学会年次学術集会
  • Place of Presentation: 東京
  • Year and Date: 2008-05-23
• [Presentation] NODマウスへのグレリン長期投与の検討 (2008)
  • Author(s): 岩倉浩
  • Organizer: 第81回日本内分泌学会学術総会
  • Place of Presentation: 青森
  • Year and Date: 2008-05-16
• [Presentation] 肥満モデル、糖尿病モデルマウスでのグレリン作用の検討 (2007)
  • Author(s): 岩倉浩
  • Organizer: 第80回日本内分泌学会学術総会
  • Place of Presentation: 東京
  • Year and Date: 2007-06-14
• [Presentation] 肥満モデル、糖尿病モデルマウスでのグレリン作用の検討 (2007)
  • Author(s): 岩倉浩
  • Organizer: 内分泌学会
  • Place of Presentation: 東京国際フォーラム
  • Year and Date: 2007-06-14