Generation of a transgenic mouse that expressing human diphtheria toxin receptor cDNA under the control of the transcription regulatory regions of ghrelin

• Project/Area Number: 19790648
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Endocrinology
• Research Institution: Kyoto University
• Principal Investigator: ARIYASU Hiroyuki Kyoto University, 大学院医学研究科, 助教 (50378650)
• Project Period (FY): 2007 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥3,690,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥390,000); Fiscal Year 2008: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2007: ¥2,000,000 (Direct Cost: ¥2,000,000)
• Keywords: グレリン / ジフテリアトキシン受容体 / トランスジェニックマウス

Research Abstract

ヒトグレリンプロモーターのコントール下にヒトジフテリアトキシン受容体を発現するトランスジェニックマウスの作成に成功した。このトランスジェニックマウスは、ジフテリアトキシン受容体を胃・十二指腸で発現しており、ジフテリアトキシン投与によってグレリン分泌細胞が減少し、血中グレリン濃度が低下することが確認できた。生後の随意の時期に循環血中グレリンを低下させうるマウスの開発に成功した。このマウスは、グレリンの生理作用を検討する上で重要なモデルマウスである。

Research Products

• [Journal Article] Efficacy of ghrelin as a therapeutic approach for age-related physiological changes. (2008)
  • Author(s): Ariyasu H, Iwakura H, Yamada G, Nakao K, Kangawa K, Akamizu T.
  • Journal Title: Endocrinology 149 Pages: 3722-3728
  • Peer Reviewed
• [Journal Article] Establishment of a novel neuroblastoma mouse model. (2008)
  • Author(s): Iwakura H, Ariyasu H, Kanamoto N, Hosoda K, Nakao K, Kangawa K, Akamizu T.
  • Journal Title: Int J Oncol. 33 Pages: 1195-1199
  • Peer Reviewed
• [Journal Article] the OA-THR Clinical Study Team. Effects of ghrelin treatment in patients undergoing total hip replacement for osteoarthritis: Different outcomes from studies in patients with cardiac and pulmonary cachexia. (2008)
  • Author(s): Akamizu T, Iwakura H, Ariyasu H, Murayama T, Sumi E, Teramukai S, Goto K, Ohnishi E, Akiyama H, Kawanabe K, Nankaku M, Ichihashi N, Tsuboyama T, Tamai K, Kataoka M, Nakamura T, Kangawa K
  • Journal Title: J Am Geriatr 56 Pages: 2363-2365
  • Peer Reviewed
• [Journal Article] Repeated administration of ghrelin to patients with functional dyspepsia: its effects on food intake and appetite. (2008)
  • Author(s): Akamizu T, Iwakura H, Ariyasu H, Hosoda H, Murayama T, Yokode M, Teramukai S, Seno H, Chiba T, Noma S, Nakai Y, Fukunaga M, Nakai Y, Kangawa K, FD Clinical Study Team.
  • Journal Title: Eur J Endocrinol 158 Pages: 491-498
  • Peer Reviewed
• [Journal Article] Efficacy of ghrelin as a therapeutic approach for age-related physiological changes (2008)
  • Author(s): Ariyasu H
  • Journal Title: Endocrinology 149 Pages: 3722-3728
  • Peer Reviewed
• [Journal Article] Effects of ghrelin treatment in patients undergoing total hip replacement for osteoarthritis : Different (2008)
  • Author(s): Akamizu T
  • Journal Title: J Am Geriatr 56 Pages: 2363-2365
  • Peer Reviewed
• [Journal Article] Repeated administration of ghrelin to patients with functional dyspepsia : its effects on food intake and (2008)
  • Author(s): Akamizu T
  • Journal Title: Eur J Endocrinol 158 Pages: 491-498
  • Peer Reviewed
• [Journal Article] Effects of ghrelin administration on decreased growth hormone status in obese animals. (2007)
  • Author(s): Iwakura H, Akamizu T, Ariyasu H, Irako T, Hosoda K, Nakao K, Kangawa K.
  • Journal Title: Am J Physiol Endocrinol Metab. 293
  • Peer Reviewed
• [Journal Article] Effects of ghrelin administration on decreased growth hormone status in obese animals (2007)
  • Author(s): Iwakura H
  • Journal Title: Am J Physiol Endocrinol Metab 293(In press)
  • Peer Reviewed
• [Presentation] 加齢に伴うソマトポーズ及び食欲低下に対するグレリン有用性の研究 (2008)
  • Author(s): 有安宏之
  • Organizer: 第81回日本内分泌学会学術総会
  • Place of Presentation: 青森市文化会館
  • Year and Date: 2008-05-18
• [Presentation] 加齢に伴うソマトポーズ及び食欲低下に対するグレリン有用性の研究 (2007)
  • Author(s): 有安宏之
  • Organizer: 第34回 日本神経内分泌学会
  • Place of Presentation: 群馬県前橋市
  • Year and Date: 2007-08-04
• [Presentation] 加齢に伴うソマトポーズ及び食欲低下に対するグレリン有用性の研究 (2007)
  • Author(s): 有安宏之
  • Organizer: 第34回日本神経内分泌学会
  • Place of Presentation: 群馬県前橋市
• [Remarks]
  • URL: http://www.kuhp.kyoto-u.ac.jp/~ghrelin/