ヒトES細胞からの骨格筋細胞の分化誘導とその内分泌臓器としての分化過程の解明 に関する研究

• Project/Area Number: 19790649
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Endocrinology
• Research Institution: Kyoto University
• Principal Investigator: SONE Masakatsu Kyoto University, 大学院医学研究科, 助教 (40437207)
• Project Period (FY): 2007 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥3,690,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥390,000); Fiscal Year 2008: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2007: ¥2,000,000 (Direct Cost: ¥2,000,000)
• Keywords: 内分泌学

Research Abstract

申請者は、これまでにヒトES細胞から誘導されたFlk1陽性細胞より平滑筋細胞を分化誘導することに成功している。また、共同研究者らは、同じくFlk1陽性細胞より心筋細胞の分化誘導に成功している。そこで、本研究では、同じFlk1陽性細胞から骨格筋細胞の分化誘導を試み、Flk1陽性細胞が3種の筋肉すべてに分化する能力を持つか検討を行ったが、一般的な骨格筋分化条件では成熟した骨格筋細胞へは分化しなかった。

Research Products

• [Journal Article] Induction and Isolation of Vascular Cells From Human Induced Pluripotent Stem Cells. (2009)
  • Author(s): Taura D, Sone M, Homma K, Oyamada N, Takahashi K, Tamura N, Yamanaka S, Nakao K.
  • Journal Title: Arterioscler Thromb Vasc Biol. May 7 [Epub ahead of print]
  • Peer Reviewed
• [Journal Article] Adipogenic differentiation of human induced pluripotent stem cells: comparison with that of human embryonic stem cells. (2009)
  • Author(s): Taura D, Noguchi M, Sone M, Hosoda K, Mori E, Okada Y, Takahashi K, Homma K, Oyamada N, Inuzuka M, Sonoyama T, Ebihara K, Tamura N, Itoh H, Suemori H, Nakatsuji N, Okano H, Yamanaka S, Nakao K.
  • Journal Title: FEBS Lett. Mar 18;583(6) [Epub 2009 Feb 27] Pages: 1029-33
  • Peer Reviewed
• [Journal Article] Close examination of steroidogenesis disorders in a DOC- and progesterone -producing adrenocortical carcinoma. (2009)
  • Author(s): Sone M, Shibata H, Homma K, Tamura N, Akahira J, Hamada S, Yahata M, Fukui N, Itoh H, Sasano H, Nakao K.
  • Journal Title: Endocrine. Feb;35(1)[Epub 2008 Nov 5] Pages: 25-33
  • Peer Reviewed
• [Journal Article] Close examinatioh of steroidogenesis disorders in a DOC-and Progesterone-Producing adrenocortical carcinoma (2009)
  • Author(s): Sone M, et.al.
  • Journal Title: Endocrine 35(1) Pages: 25-33
  • Peer Reviewed
• [Journal Article] Adipogenic differentiation of human induced pluripotent stem cells : comparison with that of human embryonic stem cells (2009)
  • Author(s): Taura D, Sone M, et.al.
  • Journal Title: FEBS Lett 583(6) Pages: 1029-33
  • Peer Reviewed
• [Journal Article] Transplantation of vascular cells derived from human embryonic stem cells contributes to vascular regeneration after stroke in mice. (2008)
  • Author(s): Oyamada N, Itoh H, Sone M, Yamahara K, Miyashita K, Park K, Taura D, Inuzuka M, Sonoyama T, Tsujimoto H, Fukunaga Y, Tamura N, Nakao K.
  • Journal Title: J Transl Med. Sep 30;6 Pages: 54-54
  • Peer Reviewed
• [Journal Article] The role of mineralocorticoid receptor expression in brain remodeling after cerebral ischemia. (2008)
  • Author(s): Oyamada N, Sone M, Miyashita K, Park K, Taura D, Inuzuka M, Sonoyama T, Tsujimoto H, Fukunaga Y, Tamura N, Itoh H, Nakao K.
  • Journal Title: Endocrinology. Aug;149(8)[Epub 2008 Apr 24] Pages: 3764-77
  • Peer Reviewed
• [Journal Article] Augmentation of neovascularization [corrected] in hindlimb ischemia by combined transplantation of human embryonic stem cells-derived endothelial and mural cells. (2008)
  • Author(s): Yamahara K, Sone M, Itoh H, Yamashita JK, Yurugi-Kobayashi T, Homma K, Chao TH, Miyashita K, Park K, Oyamada N, Sawada N, Taura D, Fukunaga Y, Tamura N, Nakao K.
  • Journal Title: PLoS ONE. Feb 27;3(2)
  • Peer Reviewed
• [Journal Article] Therapeutic potential of atrial natriuretic peptide administration on peripheral arterial diseases. (2008)
  • Author(s): Park K, Itoh H, Yamahara K, Sone M, Miyashita K, Oyamada N, Sawada N, Taura D, Inuzuka M, Sonoyama T, Tsujimoto H, Fukunaga Y, Tamura N, Nakao K.
  • Journal Title: Endocrinology. Feb;149(2)[Epub 2007 Nov 8] Pages: 483-91
  • Peer Reviewed
• [Journal Article] Transplantation of vascular cells derived from human embryonic stemcells contributes to vascular regeneration after stroke in mice (2008)
  • Author(s): Oyamada N, Sone M, et.al.
  • Journal Title: J Transl Med 6 Pages: 54-54
  • Peer Reviewed
• [Journal Article] Therapeutic Potential of Atrial Nariuretic Peptide Administration on Peripheral Arterial Diseases (2008)
  • Author(s): Park K, Itoh H, Sone M, et. al.
  • Journal Title: Endocrinology 149(2) Pages: 483-91
  • Peer Reviewed
• [Journal Article] Augmentation of neovascularizaiton in hindlimb ischemia by combined transplantation of human embryonic stem cells-derived endothelial and mural cells (2008)
  • Author(s): Yamahara K, Sone M, et. al.
  • Journal Title: PLoS ONE 3(2) Pages: 1666-1666
  • Peer Reviewed
• [Journal Article] Pathway for differentiation of human embryonic stem cells to vascular cell components and their potential for vascular regeneration. (2007)
  • Author(s): Sone M, Itoh H, Yamahara K, Yamashita JK, Yurugi-Kobayashi T, Nonoguchi A, Suzuki Y, Chao TH, Sawada N, Fukunaga Y, Miyashita K, Park K, Oyamada N, Sawada N, Taura D, Tamura N, Kondo Y, Nito S, Suemori H, Nakatsuji N, Nishikawa S, Nakao K.
  • Journal Title: Arterioscler Thromb Vasc Biol. Oct;27(10)[Epub 2007 Sep 13] Pages: 2127-34
  • Peer Reviewed
• [Journal Article] Pathway for differentiation of human embryonic stem cells to vascular cell components and their potential for vascular regeneration (2007)
  • Author(s): Sone M, et. al.
  • Journal Title: Arterioscler. Thromb. Vasc. Biol. 27(10) Pages: 2127-34
  • Peer Reviewed
• [Book] 医学のあゆみ, 2007, 220(2) (2007)
  • Author(s): 曽根正勝, 中尾一和
  • Publisher: 医歯薬出版株式会社
• [Book] 医学のあゆみ Vol.220 No.2 (2007)
  • Author(s): 曽根正勝、中尾一和
  • Total Pages: 4208
  • Publisher: 医歯薬出版株式会社