Analysis of functions of Spred-1 in melanocyte stem cells and common molecular mechanisms in tissue-specific stem cell regulations.

• Project/Area Number: 19790775
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Dermatology
• Research Institution: Kanazawa University
• Principal Investigator: TADOKORO Yuko Kanazawa University, がん研究所, 助教 (00447343)
• Project Period (FY): 2007 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥3,780,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥480,000); Fiscal Year 2008: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2007: ¥1,700,000 (Direct Cost: ¥1,700,000)
• Keywords: 再生 / 発生 / 分化 / 幹細胞 / 皮膚 / 色素

Research Abstract

本研究課題では、様々な組織幹細胞システムの制御機構における共通分子基盤の解明を目的とし、Spred-1に着目して造血幹細胞と色素幹細胞システムを中心に解析を行なった。Spred-1欠損マウスの解析から、血液系ではSpred-1はサイトカインである幹細胞因子(SCF)による刺激を抑制的に制御することによって造血幹細胞とその維持・分化の場所であるニッチとの親和性を調節していることが示唆された。一方色素系でも同様にSpred-1欠損マウスを用いた解析を行なった結果、色素細胞が過剰に増殖していることを明らかにした。

Research Products

• [Journal Article] Structural and functional changes of sulfated glycosaminoglycans in Xenopus laevis during embryogenesis. (2009)
  • Author(s): Shuhei Yamada, Masako Onishi, Reiko Fujinawa, Yuko Tadokoro, Koji Okabayashi, Makoto Asashima and Kazuyuki Sugahara
  • Journal Title: Glycobiology 19(5) Pages: 488-498
  • NAID: 120002087034
  • Peer Reviewed
• [Journal Article] Structural and functional changes of sulfated glycosaminoglycans in Xenopus laevis during embryogenesis (2009)
  • Author(s): Shuhei Yamada
  • Journal Title: Glycobiology 19(5) Pages: 488-498
  • Peer Reviewed
• [Journal Article] De novo DNA methyltransferase is essential for self-renewal, but not for differentiation, in hematopoietic stem cells. (2007)
  • Author(s): Yuko Tadokoro, Hideo Ema, Masaki Okano, En Li, and Hiromitsu Nakauchi
  • Journal Title: The Journal of Experimental Medicine 204(4) Pages: 715-722
  • Peer Reviewed
• [Journal Article] De novo DNA methyltransferase is essential for self-renewal, but not for differentiation, in hematopoietic stem cells (2007)
  • Author(s): Yuko Tadokoro
  • Journal Title: The Journal of Experimental Medicine 204(4) Pages: 715-722
  • Peer Reviewed
• [Journal Article] Adult mouse hematopoietic stem cells: purification and single-cell assays (2007)
  • Author(s): Hideo Ema
  • Journal Title: Nature Protocols 1 Pages: 2979-2987
  • Peer Reviewed
• [Journal Article] Lnk negatively regulates self-renewal of hematopoietic stem cells by modifying thrombopoietin-mediated signal transduction (2007)
  • Author(s): Jun Seita
  • Journal Title: Proceedings of the National Academy of Sciences of the United States of America 104(7) Pages: 2349-2354
  • Peer Reviewed
• [Patent(Industrial Property Rights)] Methods for inhibiting hair depigmentation and hair loss (2008)
  • Inventor(s): 西村栄美、清水宏、谷村心太郎、田所優子、澤村大輔、西江渉
  • Industrial Property Rights Holder: 西村栄美、清水宏、谷村心太郎、田所優子、澤村大輔、西江渉
  • Filing Date: 2008-02-22
  • Overseas
• [Patent(Industrial Property Rights)] Methods for inhibiting hair depigmentation and hair loss (2008)
  • Inventor(s): 西村栄美、清水宏、谷村心太郎、田所優子、澤村大輔、西江渉
  • Industrial Property Rights Holder: 西村栄美、清水宏、谷村心太郎、田所優子、澤村大輔、西江渉
  • Filing Date: 2008-02-22
  • Overseas
• [Patent(Industrial Property Rights)] XVII型コラーゲンに関する分化抑制剤 (2007)
  • Inventor(s): 西村栄美、清水宏、谷村心太郎、田所優子、澤村大輔、西江渉
  • Industrial Property Rights Holder: 西村栄美、清水宏、谷村心太郎、田所優子、澤村大輔、西江渉
  • Industrial Property Number: 2007-202319
  • Filing Date: 2007-08-02