Functional analysis of a MAP4K, MINK in adhesion and movement of keratinocytes

• Project/Area Number: 19790794
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Dermatology
• Research Institution: University of the Ryukyus
• Principal Investigator: TAKEI Kimiko University of the Ryukyus, 医学研究科, 助教 (90325861)
• Project Period (FY): 2007 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥3,750,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥450,000); Fiscal Year 2008: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000); Fiscal Year 2007: ¥1,800,000 (Direct Cost: ¥1,800,000)
• Keywords: ケラチノサイト / 細胞間接着 / MAPKKKキナーゼ / Rap2

Research Abstract

MINKは低分子量G蛋白質Rap2により活性化される。MINK結合蛋白として足場蛋白TANC1を同定した。MINKはRap2により活性化され、TANC1をリン酸化した。Rap2、MINK、TANC1およびTANC1と結合するPSD95やSAP97等の足場蛋白もケラチノサイト細胞株の細胞間接着部位に局在したので、MINK系は足場蛋白との結合やリン酸化によって接着や運動を制御する可能性がある。

Research Products

• [Journal Article] Rap2 function requires palmitoilation and recycling endosome localization (2009)
  • Author(s): Uechi, Y., Bayarjargal, M., Umikawa, M., Oshiro, M., Takei, K., Yamashiro, Y., Asato, T., Endo, S., Misaki, R., Taguchi, T., Kariya, K.
  • Journal Title: Biochem. Biophys. Res. Commun. 378 Pages: 732-737
  • Peer Reviewed
• [Journal Article] Rap2 function requires palmitoylation and recycling endosome localization (2009)
  • Author(s): Uechi Y, et.al.
  • Journal Title: Biochemical and Biophysical Research Communications 378 Pages: 732-737
  • Peer Reviewed
• [Journal Article] MINK is a Rap2 effector for phosphorylation of the postosynaptic scaffold protein TANC1 (2008)
  • Author(s): Nonaka,H., Takei,K., Umikawa,M., Oshiro M., Kuninaka, K., Bayarjargal, M., Asato, T., Yamashiro, Y., Uechi, Y., Endo, S., Suzuki, T., Kariya, K.
  • Journal Title: Biochem. Biophys. Res. Commun. 377 Pages: 573-578
  • Peer Reviewed
• [Journal Article] MINK is a Rap2 effector for phosphorylation of the postsynaptic scaffold protein TANC1 (2008)
  • Author(s): Nonaka H/Takei K (co-1st), et.al.
  • Journal Title: Biochemical and Biophysical Research Communications 377 Pages: 573-578
  • Peer Reviewed
• [Presentation] Localization of Rap2 in recycling endosome in a palmitoylation-dependent manner (2008)
  • Author(s): Bayarjargal M, et.al.
  • Organizer: 第31回日本分子生物学会年会
  • Place of Presentation: 神戸国際展示場
  • Year and Date: 2008-12-09