Regulation of dendritic cell functions with C-type lectin paired immunoreceptors,DCIR and DCAR

• Project/Area Number: 19790800
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Dermatology
• Research Institution: Wakayama Medical University
• Principal Investigator: ANAZAWA Nobuo Wakayama Medical University, 医学部, 講師 (90343227)
• Project Period (FY): 2007 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥3,780,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥480,000); Fiscal Year 2008: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2007: ¥1,700,000 (Direct Cost: ¥1,700,000)
• Keywords: DCIR / DCAR / 樹状細胞 / 皮膚

Research Abstract

In vitroの実験により、R&D社の抗DCIR抗体とBD社の33D1抗体はそれぞれDCIR1とDCIR2に特異的だが、R&D社の抗DCAR抗体はDCAR2とDCIR1の双方を認識することが判明した.従って現時点で手元にある抗体でマウスDCARを特異的に認識し刺激を導入する方法を確立できず、DCIRとDCARによる皮膚を場とする樹状細胞機能制御機構を明らかにすることはできなかった.

Research Products

• [Journal Article] No involvement of non-synonymous TLR2 polymorphisms in Japanese leprosy patients (2009)
  • Author(s): Mikita N, Kanazawa N, Ozaki M, Kosaka M, Ishi N, Nishimura H, Furukawa F
  • Journal Title: Journal of Dermatological Science 54 Pages: 48-49
  • NAID: 10025333204
  • Peer Reviewed
• [Journal Article] Role of NOD2 genotype in the clinical phenotype of Blau syndrome and early-onset sarcoidosis (2009)
  • Author(s): Okafuji I, Nishikomori R, Kanazawa N, Kambe N, Fujisawa A, Yamazaki S, Saito M, Yoshioka T, Kawai T, Sakai H, Tanizaki H, Heike T, Miyachi Y, Nakahata T
  • Journal Title: Arthritis and Rheumatism 60 Pages: 242-250
  • Peer Reviewed
• [Journal Article] Cutaneous polyarteritis nodosa : revisiting its definition and diagnostic criteria (2009)
  • Author(s): Nakamura T, Kanazawa N, Ikeda T, Yamamoto Y, Nakabayashi K, Ozaki S, Furukawa F
  • Journal Title: Archives of Dermatological Research 301 Pages: 117-121
  • Peer Reviewed
• [Journal Article] No involvement of non-synonymous TLR2 polymorphisms in Japanese leprosy patients. (2009)
  • Author(s): Mikita N, Kanazawa N, Ozaki M, Kosaka M, Ishii N, Nishimura H. Furukawa F
  • Journal Title: Journal of Dermatological Science 54 Pages: 48-49
  • NAID: 10025333204
  • Peer Reviewed
• [Journal Article] Role of NOD2 genotype in the clinical phenotype of Blau syndrome and early-onset sarcoidosis (2009)
  • Author(s): Okafuji I, Nishikomori R, Kanazawa N, Kambe N, Fujisawa A, Yamazaki S, Saito M, Yoshioka T, Kawai T, Sakai H, Tanizaki H, Heike T, Mivachi Y, Nakahata T
  • Journal Title: Arthritis and Rheumatism 60 Pages: 242-250
  • Peer Reviewed
• [Journal Article] The expression of histamine receptors in skin lesions of MRL/MP-lpr/lpr mice (2008)
  • Author(s): Yoshimasu T, Kanazawa N, Mikita N, Furukawa F
  • Journal Title: Open Dermatology Journal 2 Pages: 83-86
  • Peer Reviewed
• [Journal Article] a case of pyoderma gangrenosum with intractable leg ulcers treated by allogeneic cultured dermal substitutes (2008)
  • Author(s): Toyozawa S, Yamamoto Y, Nishide T, Kishioka A, Kanazawa N, Matsumoto Y, Kuroyanagi Y, Furukawa F. Case report
  • Journal Title: Dermatology Online Journal 14 Pages: 17-17
  • Peer Reviewed
• [Journal Article] Guideline for the diagnosis and treatment of scabies in Japan (second edition) (2008)
  • Author(s): Executive Committee of Guideline for the Diagnosis (Ishii N, Asahina A, Amagai M, Iijima M, Ishikawa O, Imamura H, Ooe M, Ohtaki N, Kato Y, Kanazawa N, Kamide R, Kanzaki T, Konohana A, Komoda M, Sugiyama N, Sekine M, Takezaki S, Tanaka M, Tamura N, Nagaoka Y, Nanko H, Hayashi M, Makigami K, Matsuda T, Yoshizumi J, Wada Y)
  • Journal Title: Journal of Dermatology 35 Pages: 378-393
  • NAID: 10024297175
  • Peer Reviewed
• [Journal Article] The expression of histamine receptors in skin lesions of MRL/M13-1pr/lpr mice (2008)
  • Author(s): Yoshimasu T, Kanazawa N, Mikita N, Furukawa F
  • Journal Title: Open Dermatology Journal 2 Pages: 83-86
  • Peer Reviewed
• [Journal Article] Case report : a case of pyoderma gangrenosum with intractable leg ulcers treated by allogeneic cultured dermal substitutes (2008)
  • Author(s): Toyozawa S, Yamamoto Y, Nishide T, Kishioka A, Kanazawa N, Matsumoto Y, Kuroyanagi Y, Furukawa F
  • Journal Title: Dermatology Online Journal 14 Pages: 17-17
  • Peer Reviewed
• [Journal Article] Autoinflammatory syndromes with a dermatological perspective (2007)
  • Author(s): Kanazawa N, Furukawa F.
  • Journal Title: Journal of Dermatology 34 Pages: 601-618
  • NAID: 10023918786
  • Peer Reviewed
• [Journal Article] Induction of PDGF-B in TCA-treated epidermal keratinocytes (2007)
  • Author(s): Yonei N, Kanazawa N, Ohtani T, Furukawa F, Yamamoto Y
  • Journal Title: Archives of Dermatological Research 299 Pages: 433-440
  • Peer Reviewed
• [Journal Article] Disease-associate d mutations in CIAS1 induce cathepsin B-dependent rapid cell death of human THP-1 monocytic cells (2007)
  • Author(s): Fujisawa A, Kambe N, Saito M, Ni-shi komori R, Tanizaki H, Kanazawa N, A dachi S, Heike T, Sagara J, Suda T, N akahata T, Miyachi Y
  • Journal Title: Blood 109 Pages: 2903-2911
  • Peer Reviewed
• [Journal Article] Dendritic cell immunorece ptors: C-type lectin receptors for pattern-recognition and signaling on antigen presenting cells (2007)
  • Author(s): Kanazawa N
  • Journal Title: Journal of Dermatological Science 45 Pages: 77-86
• [Journal Article] C-type lectin receptors for pattern-recognition and signaling on antigen-presenting cells (2007)
  • Author(s): Kanazawa N
  • Journal Title: Journal of Dermatological Science 45 Pages: 77-86
  • Peer Reviewed
• [Journal Article] Autoinflammatory syndromes with a dermatological perspective (2007)
  • Author(s): Kanazawa N, Furukawa F
  • Journal Title: Jotimal of Dermatology 34 Pages: 601-618
  • NAID: 10023918786
  • Peer Reviewed
• [Journal Article] Induction of PDGF B in TCA-treated epidermal keratinocytes. (2007)
  • Author(s): Yonei N, Kanazawa N, Ohtani T, Furukawa F, Yamamoto Y
  • Journal Title: Archives of Dermatological Research 299 Pages: 433-440
  • Peer Reviewed
• [Journal Article] Disease-associated mutations in CIASl induce cathepsin B-dependent rapid cell death of human THP-1 monocytic cells (2007)
  • Author(s): Fujisawa A, Kambe N, SaitoM, Nishikomori R, Tanizaki H, Kanazawa N, Adachi S, HeikeT, Sagara J, Suda T, NakahataT, Miyachi Y
  • Journal Title: Blood 109 Pages: 2903-2911
  • Peer Reviewed
• [Presentation] Blau症候群 (2008)
  • Author(s): 金澤伸雄
  • Organizer: 第1回自己炎症疾患研究会
  • Place of Presentation: 東京
  • Year and Date: 2008-07-10
• [Presentation] 日本のハンセン病における細菌センター分子TLR2,DC-SIGN, NOD1,NOD2 の遺伝子的関与. (2008)
  • Author(s): 金澤伸雄、三木田直哉、李洪錦、中谷友美、尾崎元昭、小坂真紀、石井則久、古川福実
  • Organizer: 第38回日本免疫学会総会・学術集会
  • Place of Presentation: 京都
• [Presentation] 家族性日本熱(中條-西村症候群):凍瘡様皮疹と限局性脂肪萎縮を伴う新しい遺伝性周期熱症候群 (2008)
  • Author(s): 金澤伸雄、古川福実、松中成浩、河野誠司、小池通夫、杉野禮俊、井田弘明
  • Organizer: 第38回日本皮膚アレルギー・接触皮膚炎学会総会学術大会
  • Place of Presentation: 大阪
• [Presentation] 若年発症サルコイドーシス・Blau症候群とNOD2変異 (2008)
  • Author(s): 金澤伸雄、岡藤郁夫、西小森隆太
  • Organizer: 第38回日本皮膚アレルギー・接触皮膚炎学会総会学術大会
  • Place of Presentation: 大阪
• [Presentation] Genetic involvement of bacterial sensor molecules, TLR2, DC-SIGN, NOD1 and NOD2, in Japanese leprosy (2008)
  • Author(s): Kanazawa N, Mikita N, Li H, Nakatani N, Ozaki M, Kosaka M, Ishii N, Furukawa F
  • Organizer: The 10th International Symposium on Dendritic Cells
  • Place of Presentation: 神戸
• [Presentation] 細菌センサー分子TLR2・DC-SIGN・NOD2の遺伝子多型のハンセン病発症への関与 (2008)
  • Author(s): 金澤伸雄、三木田直哉、尾崎元昭、小坂眞紀、石井則久、古川福実
  • Organizer: 第20回日本アレルギー学会春期臨床大会
  • Place of Presentation: 東京
• [Presentation] 細菌センサー分子TLCR2・DC-SIGN・NOD2の遺伝子多型のハンセン病発症への関与 (2008)
  • Author(s): 金澤伸雄、三木田直哉、尾崎元昭、小坂眞紀、石井則久、西村泰行、古川福実
  • Organizer: 第81回日本ハンセン病学会総会・学術大会
  • Place of Presentation: 熊本
• [Presentation] Genetic involvement of bacterial sensor molecules (2008)
  • Author(s): Kanazawa N, Mikita N, Nakatani N, Ozaki M, Kosaka M, Ishii N, Nishimura H, Furukawa F
  • Organizer: TLR2, DC-SIGN and NOD2,in Hansen's disease. International Investigative Dermatology 2008
  • Place of Presentation: 京都
• [Presentation] Autoinflammatory syndromes with a dermatological perspective (2008)
  • Author(s): Kanazawa N
  • Organizer: The 2nd International Conference on Cutaneous Lupus Erythematosus
  • Place of Presentation: 京都
• [Presentation] 自己炎症疾患とIL-1受容体アンタゴニスト(IL-1Ra)療法 (2008)
  • Author(s): 金澤伸雄
  • Organizer: 第107回日本皮膚科学会総会
  • Place of Presentation: 京都
• [Presentation] Familial Japanese fever (Nakajo-Nishimura syndrome): a novel hereditary periodic fever with partial lipodystrophy and a search for LPIN gene mutations (2008)
  • Author(s): Kanazawa N, Mikita N, Furukawa F, Nishimura H, Kawano S, Matsunaka M, Koike M, Sugino H
  • Organizer: The Fifth International Congress on Familial Mediterranean Fever and Systemic Autoinflammatory Diseases
  • Place of Presentation: ローマ(イタリア)
• [Presentation] Familial Japanese fever (Nakajo-Nishimura syndrome) : a novel hereditary periodic fever with partial lipodystrophy and a search for LPIN gene mutations (2008)
  • Author(s): Kanazawa N, Mikita N, Furukawa F, Nishimura H, Kawano S, Matsunaka M, Koike M, Sugino H
  • Organizer: The Fifth International Congress on Familial Mediterranean Fever and Systemic Autoinflammatory Diseases
  • Place of Presentation: ローマ(イタリア)
• [Presentation] Genetic involvement of bacterial sensor molecules, TLR2, DC-SIGN and NOD2, in Hansen's disease (2008)
  • Author(s): Kanazawa N, Mikita N, Nakatani N, Ozaki M, Kosaka M, Ishii N, Nishimura H. Furukawa F
  • Organizer: International Investigative Dermatology 2008
  • Place of Presentation: 京都
• [Presentation] Genetic involvement of bacterial sensor molecules, TLR2, DC-SIGN, NODI and NOD2, in Japanese leprosy (2008)
  • Author(s): Kanazawa N, Mikita N, Li H, Nakatani N, Ozaki M, Kosaka M, Ishii N, Furukawa F
  • Organizer: The 10th International Symposium on Dendritic Cells
  • Place of Presentation: 神戸
• [Presentation] 細菌センサー分子TLR2・DC-SIGN・NOD2の遺伝子多型のハンセン病発症への関与 (2008)
  • Author(s): 金盤雄、三木田直哉、尾崎元昭、小坂眞紀、石井則久、西村泰行、古川福実
  • Organizer: 第81回日本ハンセン病学会総会・学術大会
  • Place of Presentation: 熊本
• [Presentation] 家族性日本熱(中條-西村症候群) : 凍瘡様皮疹と限局性脂肪萎縮を伴う新しい遺伝性周期熱症候群 (2008)
  • Author(s): 金澤伸雄、古川福実、松中成浩、河野誠司、小池通夫、杉野禮俊、井田弘明
  • Organizer: 第38回日本皮膚アレルギー・接触皮膚炎学会総会学術大会
  • Place of Presentation: 大阪
• [Presentation] 日本のハンセン病における細菌センター分子TLR2, DC-SIGN, NOD1, NOD2の遺伝子的関与 (2008)
  • Author(s): 金澤伸雄、三木田直哉、李洪錦、中谷友美、尾崎元昭、小坂真紀、石井則久、古川福実
  • Organizer: 第38回日本免疫学会総会・学術集会
  • Place of Presentation: 京都
• [Presentation] Analysis of TLR2 gene polymorphisms in Hansen's disease (2007)
  • Author(s): Kanazawa N, Mikita N, Ozaki M, Kosaka M, Ishii N, Nishimura H, Furukawa F
  • Organizer: The 4th World Association of Sarcoidosis and Other Granulomatous Disorders
  • Place of Presentation: 東京
  • Year and Date: 2007-10-19
• [Presentation] 若年発症サルコイドーシスの臨床像とNOD2遺伝子変異の相関 (2007)
  • Author(s): 金澤伸雄、岡藤郁夫、谷崎英昭、神戸直智、西小森隆太、中畑龍俊、宮地良樹
  • Organizer: 第44回日本臨床分子医学会学術集会
  • Place of Presentation: 和歌山
• [Presentation] ハンセン病におけるTLR2遺伝子変異の検索 (2007)
  • Author(s): 金澤伸雄、三木田直哉、尾崎元昭、小坂眞紀、石井則久、西村泰行、古川福実
  • Organizer: 第80回日本ハンセン病学会総会・学術大会
  • Place of Presentation: 横浜
• [Presentation] ハンセン病の免疫学・分子生物学 (2007)
  • Author(s): 金澤伸雄
  • Organizer: 第106回日本皮膚科学会総会
  • Place of Presentation: 横浜
• [Presentation] 家族性日本熱(中条-西村症候群):限局性脂肪萎縮を伴う新しい遺伝性周期熱症候群とLPIN遺伝子の解析 (2007)
  • Author(s): 金澤伸雄、米井希、河野誠司、西村泰行、古川福実、樋口隆造、飯塚忠史、小池通夫、松中成浩、杉野禮俊
  • Organizer: 第32回日本研究皮膚科学会学術大会・総会
  • Place of Presentation: 横浜
• [Presentation] Analysis of TLR2 gene polymorphisms in Hansen's disease. (2007)
  • Author(s): Kanazawa N, Mikita N, Ozaki M, Kosaka M, Ishii N, Nishimura H, Furukawa F
  • Organizer: The 4th World Association of Sarcoidosis and Other Granulomatous Disorders
  • Place of Presentation: 東京
• [Presentation] サルコイド様類上皮細胞肉芽腫からなるアレルギー性ピアス肉芽腫 (2007)
  • Author(s): 金澤 伸雄、 西山 瑞穂、 山本 有紀、 古川 福実
  • Organizer: 第37回日本皮膚アレルギー・接触皮膚炎学会総会学術大会
  • Place of Presentation: 名古屋