Expression analysis of N-Myc downstream regulated gene 2 (Ndrg2) using the embryonic spinal cord and the adult brain

• Project/Area Number: 19790849
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Psychiatric science
• Research Institution: National Center of Neurology and Psychiatry
• Principal Investigator: TAKAHASHI Kou National Center of Neurology and Psychiatry, 老人精神保健部, 外来研究員 (20415582)
• Project Period (FY): 2007 – 2009
• Project Status: Completed (Fiscal Year 2009)
• Budget Amount: ¥3,710,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥510,000); Fiscal Year 2009: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2008: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2007: ¥1,500,000 (Direct Cost: ¥1,500,000)
• Keywords: 精神薬理 / 抗うつ薬 / アストロサイト / 神経幹細胞 / 発生 / 分化 / 電気けいれん / microarray / real time PCR法 / western blot法 / real time PCR 法 / western blot 法

Research Abstract

We have reported that the expression of Ndrg2 was decreased after chronic antidepressant treatment and electroconvulsive treatment in rat frontal cortex. However, roles of Ndrg2 in the central nervous system were poorly understood. In the present study, we demonstrated that the expression of Ndrg2 was detected in neuronal stem cells and astrocyte in both the embryonic spinal cord and the adult brain. These results may indicate that Ndrg2 involved in the mechanisms of proliferation and differentiation in neuronal stem cells.

Research Products

• [Journal Article] Neuroserpin is expressed in early stage of neurogenesis in adult rat hippocampus. (2010)
  • Author(s): Yamada, M., Takahashi, K., Ukai, W., Hashimoto, E., Saito, T., Yamada M.
  • Journal Title: Neuroreport 21(2) Pages: 138-142
  • Peer Reviewed
• [Journal Article] Neuroserpin is expressed in early stage of neurogenesis in adult rat hippocampus. (2010)
  • Author(s): Yamada, M., Takahashi, K., Ukai, W, Hashimoto, E., Saito, T., Yamada M.
  • Journal Title: Neuroreport 21 Pages: 138-142
  • Peer Reviewed
• [Journal Article] Induction of the transcription factor Math2 and its target gene Prg1 after chronic treatment with selective serotonin reuptake inhibitors in rat brain. (2009)
  • Author(s): Yamada, M., Shida, Y., Takahashi, K., Yamada M.
  • Journal Title: J. Mental Health 55 Pages: 103-109
  • Peer Reviewed
• [Journal Article] Induction of the transcription factor Math2 and its target gene Prg l after chronic treatment with selective serotonin reuptake inhibitors in ratbrain. (2009)
  • Author(s): Yamada, M., Shida, Y., Takahashi, K., Yamada M.
  • Journal Title: J.Mental Health 55 Pages: 103-109
  • Peer Reviewed
• [Journal Article] Gene expression profiling reveals complex changes in the olfactory bulbectomy model of depression after chronic treatment with antidepressants. (2008)
  • Author(s): Takahashi, K., Saitoh, A., Yamada, M., Maruyama, Y., Hirose, N., Kamei, J., Yamada, M.
  • Journal Title: J Pharmacol. Sci. 108(3) Pages: 320-334
  • NAID: 10024593145
  • Peer Reviewed
• [Journal Article] Prg1 is regulated by the basic helix-loop-helix transcription factor Math2. (2008)
  • Author(s): Yamada, M., Shida Y., Takahashi, K., Tanioka T., Nakano Y., Tobe T., Yamada, M.
  • Journal Title: J Neurochem 106(6) Pages: 2375-2384
  • Peer Reviewed
• [Journal Article] Ubiquitin ligase Kf-1 is involved in the endoplasmic reticulum-associated degradation pathway. (2008)
  • Author(s): Maruyama, Y., Yamada, M., Takahashi, K., Yamada, M.
  • Journal Title: Biochem. Biophys. Res. Comm. 374 Pages: 737-741
  • Peer Reviewed
• [Journal Article] Antidepressant-like effects of the delta-opioid receptor agonist SNC80 ([(+)-4-[(alphaR)-alpha-[(2S, 5R)-2, 5-di methyl-4-(2-propenyl)-1-pi perazinyl]-(3-methoxyphenyl)methyl]-N, N -diethylbenzamide] in an olfactory bulbectomized rat model. (2008)
  • Author(s): Saitoh, A., Yamada, M., Yamada, M., Takahashi K., Yamaguchi, K., Murasawa, H., Nakatani, A., Tatsumi, Y., Hirose, N., Kamei, J.
  • Journal Title: Brain research 1208 Pages: 160-169
  • Peer Reviewed
• [Journal Article] Gene expression profiling reveals complex changes in the olfactory bulbectomy model of depression after chronic treatment with antidepressants (2008)
  • Author(s): Takahashi, K
  • Journal Title: J Pharmacol. Sci. 108 Pages: 320-334
  • NAID: 10024593145
  • Peer Reviewed
• [Journal Article] Prg1 is regulated by the basic helix-loop-helix transcription factor Math2 (2008)
  • Author(s): Yamada, M
  • Journal Title: J Neurochem 106 Pages: 2375-2384
  • Peer Reviewed
• [Journal Article] Ubiquitin ligase Kf-1 is involved in the endoplasmic reticulum-associated degradation pathway (2008)
  • Author(s): Maruyama, Y
  • Journal Title: Biochem. Biophys. Res. Comm 374 Pages: 737-741
  • Peer Reviewed
• [Presentation] A glutamate release inhibitor rapidly attenuates hyperemotional responses in OBX rats. (2009)
  • Author(s): Inagaki, M., Takahashi, K., Saitoh, A., Yamada, M., Iwai, T., Nakaani, A., Murasawa, H., Yoshida, M., Yamaguchi, K., Yamada, M.
  • Organizer: The 1st Meeting of the Asian College of Neuropsychopharmacology
  • Place of Presentation: 国立京都国際会館(京都府)
  • Year and Date: 2009-11-13
• [Presentation] An antidepressant related transcription factor Math2 regulates Prgl expression and involves in neurite differentiation. (2009)
  • Author(s): Yamada, M., Yamada, M., Shida Y., Takahashi, K., Tanioka T., Nakano Y., Tobe T.
  • Organizer: The 1st Meeting of the Asian College of Neuropsychopharmacology
  • Place of Presentation: 国立京都国際会館(京都府)
  • Year and Date: 2009-11-13
• [Presentation] A glutamate release inhibitor rapidly attenuates hyperemotional responses in OBX rats. (2009)
  • Author(s): Inagaki, M., Takahashi, K., Saitoh, A., Yamada, M., Iwai, T., Nakaani, A., Murasawa, H., Yoshida, M., Yamaguchi, K., Yamada, M.
  • Organizer: The 1st Meeting of the Asian College of Neuropsychopharmacology
  • Place of Presentation: Kyoto International Conference Center
• [Presentation] An antidepressant related transcription factor Math2 regulates Prg1 expression and involves in neurite differentiation. (2009)
  • Author(s): Yamada, M., Yamada, M., Shida Y., Takahashi, K., Tanioka T., Nakano Y., Tobe T.
  • Organizer: The 1st Meeting of the Asian College of Neuropsychopharmacology
  • Place of Presentation: Kyoto International Conference Center
• [Presentation] Chronic treatment with a selective opioid-delta agonist SNC80 affects the expression of excitatory amino-acid transporter genes in frontal cortex of olfactory bulbectomized rat. (2009)
  • Author(s): Saitoh, A., Yamada, M., Takahashi, K., Iwai, T., Inagaki, M., Yamada, M.
  • Organizer: The 1st Meeting of the Asian College of Neuropsychopharmacology
  • Place of Presentation: Kyoto International Conference Center
• [Presentation] The RING finger domain of Kf-l is required for ubiquitin ligase activity and subcellular redistribution (2007)
  • Author(s): 丸山 良亮, 山田 美佐, 高橋 弘, 山田 光彦
  • Organizer: BMB2007
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 2007-12-14
• [Presentation] 抗うっ薬の作用メカニズムに関わる転写因子ADRG701が制御する下流遺伝子の探索 (2007)
  • Author(s): 志田 美子, 高橋 弘, 山田 美佐, 中野 泰子, 戸部 激, 山田 光彦
  • Organizer: BMB2007
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 2007-12-14
• [Presentation] The RING finger domain of Kf-1 is required for ubiquitin ligase activity and subcellular redistribution. (2007)
  • Author(s): 丸山良亮, 山田美佐, 高橋弘, 山田光彦
  • Organizer: BMB2007
  • Place of Presentation: 横浜
• [Presentation] 抗うつ薬の作用メカニズムに関わる転写因子ADRG701が制御する下流遺伝子の探索 (2007)
  • Author(s): 志田美子, 高橋弘, 山田美佐, 中野泰子, 戸部敞山田光彦
  • Organizer: BMB2007
  • Place of Presentation: 横浜
• [Remarks]
  • URL: http://www.ncnp.go.jp/nimh/rojin/
• [Remarks]
  • URL: http://www.ncnp.go.jp/nimh/rojin/
• [Remarks]
  • URL: http://www.ncnp.go.jp/nimh/rojin/
• [Remarks]
  • URL: http://www.ncnp.go.jp/nimh/rojin/