The effect on cell motility by the genes regulated by epigecetic factor

• Project/Area Number: 19790935
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Digestive surgery
• Research Institution: Chiba University
• Principal Investigator: ARAI Makoto Chiba University, 医学部附属病院, 助教 (30396684)
• Project Period (FY): 2007 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥3,750,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥450,000); Fiscal Year 2008: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000); Fiscal Year 2007: ¥1,800,000 (Direct Cost: ¥1,800,000)
• Keywords: エピジェネティクス / 細胞運動能 / マイクロアレイ / 異常メチル化 / 発現解析 / Boyden Chamber / C15orf48 / 細胞浸潤能

Research Abstract

食道癌由来培養細胞に脱メチル化剤を投与することによって、発現増加を認めた遺伝子をマイクロアレイ解析によって絞り込み、その中で、chromosome 15 open reading frame 48(C15orf48)に注目した。この遺伝子のプロモーター領域の異常メチル化を確認し、細胞運動能との関連を明らかにした。以上より、機能不明の異常メチル化により発現が制御されるC15orf48遺伝子の機能解析を行い、細胞運動能との関連性が深いことを明らかにした。

Research Products

• [Journal Article] Analysis of the methylation status of genes up-regulated by the demethylating agent, 5-aza-2'-deoxycytidine, in esophageal squamous cell carcinoma (2008)
  • Author(s): 新井誠人, 他8名
  • Journal Title: Oncol Rep 20(2) Pages: 405-412
  • Peer Reviewed
• [Journal Article] Analysis of the methylation status of genes up-regulated by the demethylating agent, 5-aza-2'-deoxycytidine, in esophageal squamous cell carcinoma (2008)
  • Author(s): 新井誠人, 他
  • Journal Title: Oncol Rep 20(2) Pages: 405-412
  • Peer Reviewed
• [Journal Article] Chromoendoscopy with indigo carmine dye added to acetic acid in the diagnosis of gastric neoplasia : a prospective comparative study (2008)
  • Author(s): 坂井雄三、新井誠人, 他
  • Journal Title: Gastrointest Endosc 68(4) Pages: 635-641
  • Peer Reviewed
• [Journal Article] Efficacy of combination therapy of antiviral and immunosuppressive drugs for the treatment of severe acute exacerbation of chronic hepatitis B (2008)
  • Author(s): 藤原慶一、新井誠人, 他
  • Journal Title: J Gastroenterol 43(9) Pages: 711-719
  • NAID: 10022609817
  • Peer Reviewed
• [Journal Article] Analysis of the methylation status of genes up-regulated by the demethylating agent, 5-aza-2'-deoxycytidine, in esophageal squamous cell carcinoma (2008)
  • Author(s): 新井誠人
  • Journal Title: Oncology Reports (In press)
  • Peer Reviewed
• [Journal Article] Integrated analysis of expression and genome alteration reveals putative amplified target genes in esophageal cancer (2007)
  • Author(s): 杉本貴史、新井誠人, 他3名
  • Journal Title: Oncol Rep 18(2) Pages: 465-72
  • Peer Reviewed
• [Journal Article] Altered gene expression by cisplatin in a human squamous cell lung carcinoma cell line (2007)
  • Author(s): 弥富真理、新井誠人, 他10名
  • Journal Title: Anticancer Res. 27(5A) Pages: 3235-43
  • Peer Reviewed
• [Presentation] 大腸癌由来培養細胞において脱メチル化剤投与により発現上昇したマイクロRNAの解析 (2008)
  • Author(s): 田中健史、新井誠人, 他
  • Organizer: 第67回日本癌学会学術総会
  • Place of Presentation: 名古屋
  • Year and Date: 2008-10-28
• [Presentation] 肝癌細胞株におけるエピジェネティク異常とマイクロRNAの関わりの検討 (2007)
  • Author(s): 新井誠人, 他
  • Organizer: 第43回日本肝臓学会総会
  • Place of Presentation: 東京
  • Year and Date: 2007-05-31
• [Presentation] 食道扁平上皮癌におけるNMES1の役割の検討 (2007)
  • Author(s): 新井誠人, 他
  • Organizer: 第93回日本消化器病学会総会
  • Place of Presentation: 青森
  • Year and Date: 2007-04-20