The biological role and signaling pathway of neuropilin-1in human pancreatic cancer

• Project/Area Number: 19790961
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Digestive surgery
• Research Institution: Nippon Medical School
• Principal Investigator: MATSUSHITA Akira Nippon Medical School, 医学部, 助教 (70449263)
• Project Period (FY): 2007 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥1,870,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥270,000); Fiscal Year 2008: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2007: ¥700,000 (Direct Cost: ¥700,000)
• Keywords: 膵癌 / neuropilin-1 / STAT5 / stat5 / Bc1-xL / apoptosis

Research Abstract

Neuropilin-1プラスミドを用いてneuropilin-1低発現の膵癌細胞COLO357にneuropilin-1を導入したところ、Np-1高発現株では抗癌剤投与によるsurvivalが増加し、apoptosisが減少した.Neuropilin-1の抗apoptosis効果にはcaspase-3の関与が考えられた.膵癌細胞株、ヒト膵癌組織を用いた検討では、膵癌においてSTAT5A、5B mRNA、蛋白の発現が確認されさらにSTAT5Bの活性化が認められたが、PANC-1を用いたSTAT-5 siRNAによる実験ではSTAT5とsurvival,apoptosisの関連は認められなかった.

Research Products

• [Journal Article] Improvement of intraoperative frozen section diagnosis in patients with biliary strictures by Levovist injection into the bile duct on color doppler ultrasonography (2008)
  • Author(s): Aimoto T, Uchida E, Kawahigashi Y, Nakamura Y, Matsushita A, Katsuno A, Chou K, Kawamoto M, Yamanaka Y, Tajiri T, Naito Z
  • Journal Title: World J Surg 32 Pages: 88-92
  • Peer Reviewed
• [Journal Article] ハムスター膵癌細胞を用いた膵癌の転移特性の解明および治療実験の意義 (2008)
  • Author(s): 内田英二,松下晃,柳健,廣井信,相本隆幸,中村慶春,福原宗久,横山正,田尻孝
  • Journal Title: 膵臓 23 Pages: 60-65
  • NAID: 10024367440
  • Peer Reviewed
• [Journal Article] Neuropilin-1 interacts with integrin beta1 and modulates pancreatic cancer cell growth (2007)
  • Author(s): Fukasawa M, Matsushita A, Korc M
  • Journal Title: survival and invasion. Cancer Biology & Therapy 6(8) Pages: 1173-80
  • Peer Reviewed
• [Journal Article] Hepatocyte growth factor-mediated cell invasion in pancreatic cancer cells is dependent on neuropilin-1 (2007)
  • Author(s): Matsushita A, Gotze T, Korc M.
  • Journal Title: Cancer Research 67(21) Pages: 10309-10316
  • Peer Reviewed
• [Journal Article] Controlled release of basic fibroblast growth factor promotes healing of the pancreaticojejunal anastomosis : a novel approach toward zero pancreatic fistula (2007)
  • Author(s): Aimoto T, Uchida E, Matsushita A, Tabata Y, Takano T, Miyamoto M, Tajiri T
  • Journal Title: Surgery 142 Pages: 734-40
  • Peer Reviewed
• [Journal Article] Neuropilin-1 interacts with integrin betal and modulates pancreatic cancer cell growth, survival and invasion. (2007)
  • Author(s): Fukasawa M, Matsushita A, Korc M
  • Journal Title: Cancer Biology & Therapy 6(8) Pages: 1173-80
  • Peer Reviewed
• [Journal Article] Hepatocyte growth factor-mediated cell invasion in pancreatic cancer cells is dependent on neuropilin-1 (2007)
  • Author(s): Matsushita A, Gotze T, Korc M
  • Journal Title: Cancer Research 67(21) Pages: 10309-10316
  • Peer Reviewed
• [Presentation] ヒト膵癌におけるSTAT5の発現、活性化についての検討 (2009)
  • Author(s): 松下晃,内田英二,宮本昌之,渡辺秀裕,鈴木成治,横山正,丸山弘,松谷毅,杉浦篤,柳健,松田明久,西佳史,新井洋紀,若林秀幸,相本隆幸,中村慶春,川本聖郎,石渡俊行,内藤善哉,笹島耕二,田尻孝
  • Organizer: 第109回日本外科学会定期学術集会
  • Place of Presentation: 福岡
• [Presentation] Neuropilin-1 association with c-Met in pancreatic cancer cells enhances hepatocyte growth factor-mediated cell invasion. (2008)
  • Author(s): Matsushita A, Gotze T, Nakamura Y , Aimoto T, Uchida E, Tajiri T, Korc M
  • Organizer: 第108回日本外科学会総会
  • Place of Presentation: 長崎
• [Presentation] Neuropilin-1の膵癌における生物学的意義、そのintegrin beta1との関連について (2008)
  • Author(s): 松下晃,川本聖郎,中村慶春,相本隆幸,横山正,石渡俊行,内藤善哉,内田英二,笹島耕二,田尻孝
  • Organizer: 第63回日本消化器外科学会総会
  • Place of Presentation: 札幌
• [Presentation] ヒト膵癌におけるNeuropilin-1とc-Met 、HGFの関連について (2008)
  • Author(s): 松下晃,内田英二,相本隆幸,中村慶春,川本聖郎,渡辺秀裕,宮本昌之,横山正,丸山弘,鈴木成治,松谷毅,柳健,石渡俊行,内藤善哉,笹島耕二,田尻孝
  • Organizer: 第39回日本膵臓学会大会
  • Place of Presentation: 横浜
• [Presentation] Neuropilin-1 interacts with integrin beta1 and modulates pancreatic cancer cell growth, survival and invasion (2008)
  • Author(s): Matsushita A, Uchida E, Nakamura Y, Aimoto T, Matsutani T, Suzuki S, Maruyama H, Yokoyama T, Miyamoto M, Watanabe H, Sasajima K, Tajiri T
  • Organizer: 第67回日本癌学会学術総会
  • Place of Presentation: 名古屋
• [Presentation] Neurpilin-1 associates with c-Met and modulates hepatocyte growth factor actions on cell invasiveness in human pancreatic cancer. (2007)
  • Author(s): Matsushita A
  • Organizer: The 38th Annual Meeting of the American Pancreatic Association
  • Place of Presentation: Chicago, IL, USA
  • Year and Date: 2007-11-01
• [Presentation] Neuropilin-1 associates with c-Met and modulates hepatocyte growth factor actions. (2007)
  • Author(s): 松下 晃
  • Organizer: 第49回日本消化器病学会大会
  • Place of Presentation: 神戸
  • Year and Date: 2007-10-20
• [Presentation] Neuropilin-1 associates with c-Met and modulates hepatocyte growth factor actions in human pancreatic cancer. (2007)
  • Author(s): 松下 晃
  • Organizer: 第66回日本癌学会学術総会
  • Place of Presentation: 横浜
  • Year and Date: 2007-10-05
• [Presentation] Neuropilin-1 associates with c-Met and modulates hepatocyte growth factor actions. (2007)
  • Author(s): 松下 晃
  • Organizer: 第63回日本消化器外科学会定期学術総会
  • Place of Presentation: 東京
  • Year and Date: 2007-07-18
• [Presentation] 膵癌の新しい治療標的としてのneuropilin-1、そのintegrin beta1との関連について (2007)
  • Author(s): 松下 晃
  • Organizer: 第38回日本膵臓学会大会
  • Place of Presentation: 福岡
  • Year and Date: 2007-06-28
• [Presentation] Neuropilin-1 associates with c-Met and modulates hepatocyte growth factor actions (2007)
  • Author(s): Matsushita A
  • Organizer: AACR annual meeting 2007
  • Place of Presentation: Los Angeles, CA, USA
  • Year and Date: 2007-04-15
• [Presentation] Suppresion of neuropilin-1 expression in PANC-1 human pancreatic cancer cells leads to attenuated growth, survival and invasion. (2007)
  • Author(s): 松下 晃
  • Organizer: 第107回日本外科学会定期学術集会
  • Place of Presentation: 大阪
  • Year and Date: 2007-04-12
• [Presentation] Supression of neuropilin-1 in PANC-1 human pancreatic cancer cells leads to attenuated growth, survival and invasion (2007)
  • Author(s): 松下晃,深澤光晴、内田英二、田尻孝、Korc M
  • Organizer: 第107回日本外科学会総会
  • Place of Presentation: 大阪
• [Presentation] Neuropilin-1 associates with c-Met and modulates hepatocyte growth factor actions (2007)
  • Author(s): Matsushita A, Gotze T, Gunn J, Korc M
  • Organizer: Annual Meeting of the American Association of Cancer Research
  • Place of Presentation: LA, USA
• [Presentation] 膵癌の新しい治療標的としてのNeuropilin-1、そのintegrin beta1との関連について (2007)
  • Author(s): 松下晃,深澤光晴,内田英二,張一光,川本聖郎,中村慶春,相本隆幸,Korc M,田尻孝
  • Organizer: 第38回日本膵臓学会大会
  • Place of Presentation: 福岡
• [Presentation] Neuropilin-1 associates with c-Met and modulates hepatocyte growth factor actions (2007)
  • Author(s): 松下晃,内田英二,張一光,川本聖郎,中村慶春,相本隆幸,Murray Korc,田尻孝
  • Organizer: 第62回日本消化器外科学会総会
  • Place of Presentation: 東京