Development of method for cardiovascular tissue engineering-Importance of median smooth muscle layer-

• Project/Area Number: 19790970
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Thoracic surgery
• Research Institution: Nagoya University
• Principal Investigator: MUTSUGA Masato Nagoya University, 医学部附属病院, 医員 (80447820)
• Project Period (FY): 2007 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥3,690,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥390,000); Fiscal Year 2008: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2007: ¥2,000,000 (Direct Cost: ¥2,000,000)
• Keywords: 再生医療 / ティッシュエンジニアリング / 血管平滑筋細胞 / TGF-b / アスコルビン酸 / メカニカルストレス / ラミニン / TGF-β

Research Abstract

再生医療では理論的には理想的な移植材料を創出しうるが、心臓血管組織の様に「強靱」かつ「しなやか」な組織再生は未だ困難である。
そこで本研究では、心臓血管組織の機械特性を担う「中膜平滑筋層の再生」を目指した研究を行った。
まず、平滑筋細胞へ分化可能な骨髄由来間葉系幹細胞を効率的に分化するための手法を検討し、培養液中の添加因子としてTGF-b1とアスコルビン酸、細胞外マトリクスとしてラミニン、動脈同様の機械刺激環境下での培養などそれぞれの有効性を発見した。
またそれらの細胞を脱細胞化組織へ磁力を用いて層状に播種する手法を開発した。さらに、中膜平滑筋層の再生を促す、生体吸収性材料を用いた血管の再生を試みた。

Research Products

• [Journal Article] A new strategy for prevention of anastomotic stricture using tacrolimus eluting biodegradable nano-fiber (2009)
  • Author(s): Mutsuga M, Narita Y, Yamawaki A, Satake M, Kaneko H, Suematsu Y, Usui A, Ueda Y
  • Journal Title: J Thorac Cardiovasc Surg 137(3) Pages: 703-709
  • Peer Reviewed
• [Journal Article] Development of novel drug-eluting biodegradable nano-fiber for prevention of post-operative pulmonary venous obstruction (2009)
  • Author(s): Mutsuga M, Narita Y, Yamawaki A, Satake M, Kaneko H, Usui A, Ueda Y
  • Journal Title: Interact Cardiovasc Thorac Surg 2009 Jan 12
  • Peer Reviewed
• [Journal Article] A new strategy for prevention of anastomotic stricture using tacrolimus eluting biodegradable nano-fiber (2009)
  • Author(s): Mutsuga M, et.al.
  • Journal Title: J Thorac Cardiovasc Surg. 137 Pages: 703-709
  • Peer Reviewed
• [Journal Article] Development of novel drug-eluting biodegradable nano-fiber for prevention of post-operative pulmonary venous obstruction (2009)
  • Author(s): Mutsuga M, et.al.
  • Journal Title: Interact Cardiovasc Thorac Surg (In press)
  • Peer Reviewed
• [Presentation] Spinal Cord Protection during Thoracoabdominal aneurysm repair: Using vascular tube for intercostal artery reimplantation (2009)
  • Author(s): Mutsuga M
  • Organizer: 17th Annual Meeting of Asian Society for Cardiovascular and Thoracic Surgery
  • Place of Presentation: Taipei, Taiwan
• [Presentation] Spinal Cord Protection during Thoracoabdominal aneurysm repair : Using vascular tube for intercostal artery reimplantation (2009)
  • Author(s): Mutsuga M, et.al.
  • Organizer: 17^<th> Annual Meeting of Asian Society for Cardiovascular and Thoracic Surgery.
  • Place of Presentation: Taipei, Taiwan
• [Presentation] Left Ventricular Restoration for severe congestive heart failure (2008)
  • Author(s): Mutsuga M
  • Organizer: 16th Annual Meetting of Asian Society for the Cardiovascular & Thoracic Surgery
  • Place of Presentation: Singapore
  • Year and Date: 2008-03-15
• [Presentation] 中膜平滑筋層の再生を目指した骨髄由来ヒト間葉系幹細胞の分化誘導制御 (2008)
  • Author(s): 六鹿雅登
  • Organizer: 第7回再生心臓血管外科治療研究会
  • Place of Presentation: 福岡
  • Year and Date: 2008-02-20
• [Presentation] 術後吻合部狭窄予防のための薬剤溶出担体(タクロリムス溶出ナノファイバー)の開発及び基礎的研究 (2008)
  • Author(s): 六鹿雅登
  • Organizer: 第61回日本胸部外科学会
  • Place of Presentation: 福岡
• [Presentation] Development of novel drug-eluting biodegradable nano-fiber for prevention of post-operative pulmonary venous obstruction (2008)
  • Author(s): Mutsuga M
  • Organizer: 21 th Annual Meeting of European Association for Cardio-Thoracic Surgery
  • Place of Presentation: Lisbon, Portugal
• [Presentation] A New Strategy for Prevention of Anastomotic Stricture Using Tacrolimus Eluting Biodegradable Nano-fiber (2008)
  • Author(s): Mutsuga M
  • Organizer: 34th Annual Meeting of Western Thoracic Surgical Association
  • Place of Presentation: Hawaii, USA
• [Presentation] A New Strategy for Prevention of Anastomotic Stricture Using Tacrolimus Eluting Biodegradable Nano-fiber (2008)
  • Author(s): Mutsuga M, et.al.
  • Organizer: 34^<th> Annual Meeting of Western Thoracic Surgicat Association.
  • Place of Presentation: Hawaii, USA
• [Presentation] Development of novel drug-eluting biodegradable nano-fiber for prevention of post-operative pulmonary venous obstruction (2008)
  • Author(s): Mutsuga M, et.al.
  • Organizer: 21^<th> Annual Meeting of European Association for Cardio-Thoracic Surgery.
  • Place of Presentation: Lisbon, Portugal
• [Presentation] Quality of life after total arch replacement with Arch-first technique in elderly patients (2007)
  • Author(s): Mutsuga M
  • Organizer: 15th Annual Meetting of Asian Society for the Cardiovascular & Thoracic Surgery
  • Place of Presentation: Chiang Mai, Thailand
  • Year and Date: 2007-05-18
• [Patent(Industrial Property Rights)] 循環器治療用綿状構造物およびその製造方法 (2008)
  • Inventor(s): 六鹿雅登、成田裕司、上田裕一、佐竹 真、兼子博章
  • Industrial Property Rights Holder: 国立大学法人名古屋大学
  • Filing Date: 2008-04-22
• [Patent(Industrial Property Rights)] 循環器治療用締状構造物およびその製造方法 (2008)
  • Inventor(s): 六鹿雅登, 他
  • Industrial Property Rights Holder: 国立大学法人名古屋大学
  • Industrial Property Number: 2008-111236
  • Filing Date: 2008-04-22