The role of Eph/ephrin in glioma invasion

• Project/Area Number: 19790992
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Cerebral neurosurgery
• Research Institution: Kanazawa University
• Principal Investigator: NAKADA Mitsutoshi Kanazawa University, 医学系, 助教 (20334774)
• Project Period (FY): 2007 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥3,570,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥270,000); Fiscal Year 2008: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2007: ¥2,400,000 (Direct Cost: ¥2,400,000)
• Keywords: 神経膠芽腫 / 浸潤 / Eph / ephrin

Research Abstract

我々はEph /ephrinチロシンキナーゼファミリーに着目し神経膠芽腫浸潤における働きとその臨床像に与える影響を検討した。その結果、EphB2/ephrin-B2シグナルが神経膠芽腫の浸潤能を亢進させ悪性化に寄与していることが明らかになった。さらに、Ephrin-A2がEphAシグナルを介して神経膠芽腫浸潤に抑制的に働き、臨床上有用な予後規定因子になりうることを示した。これらのことからEphA/ephrin-AとEphB/ephrin-Bは神経膠芽腫の浸潤において逆の作用を有し両者のシグナルバランスで浸潤が制御されている可能性が示された。

Research Products

• [Journal Article] Role of Rac1-regulated signaling in medulloblastoma invasion. (2009)
  • Author(s): Zavarella S, Nakada M, 他6名
  • Journal Title: Journal of Neurosurg : Pediatrics (in press)
  • Peer Reviewed
• [Journal Article] Potential effect of glycogen synthase kinase 3β inhibition against human glioblastoma. (2009)
  • Author(s): Miyashita K, Kawakami K, Nakada M, 他6名
  • Journal Title: Clin Can Res 15 Pages: 887-897
  • Peer Reviewed
• [Journal Article] 脳腫瘍浸潤の分子機構 (2009)
  • Author(s): 中田光俊, 佐谷秀行
  • Journal Title: 脳21 12 Pages: 16-20
  • Peer Reviewed
• [Journal Article] Potential effect of glycogen synthase kinase 3β inhibition against hurman glioblastoma (2009)
  • Author(s): Miyashita K, Kawakami K, Nakada M, et.al.
  • Journal Title: Clin Can Res 15 Pages: 887-897
  • Peer Reviewed
• [Journal Article] Role of Racl-regulated signaling in medulloblastoma invasion (2009)
  • Author(s): Zavarella S, Nakada M, et.al.
  • Journal Title: Journal of Neurosurg : Pediatrics (in press)
  • Peer Reviewed
• [Journal Article] The guanine nucleotide exchange factors Trio, Ect2, and Vav3 mediate the invasive behavior of glioblastoma. (2008)
  • Author(s): Salhia B, Tran NL, Chan A, Wolf A, Nakada M, 他6名
  • Journal Title: Am J Pathol 173 Pages: 1828-1838
  • Peer Reviewed
• [Journal Article] Autotaxin : a secreted autocrine /paracrine factor that promotes glioma invasion. (2008)
  • Author(s): Hoelzinger DB, Nakada M, 他4名
  • Journal Title: Journal of Neuro-Oncology 86 Pages: 297-309
  • Peer Reviewed
• [Journal Article] Glioma cells on the run - the migratory transcriptome of 10 human glioma cell lines. (2008)
  • Author(s): Demuth T, Rennert JL, Hoelzinger DB, Reavie LB, Nakada M, 他13名
  • Journal Title: BMC Genomics 9 Pages: 54-54
  • Peer Reviewed
• [Journal Article] Role of myosin II activity and the regulation of myosin light chain phosphorylation in astrocytomas. (2008)
  • Author(s): Salhia B, Hwang JH, Smith CA, Nakada M, Rutka F, Symons M, Rutka JT
  • Journal Title: Cell Motility and the Cytoskeleton 65 Pages: 12-24
  • Peer Reviewed
• [Journal Article] 神経膠腫の浸潤機構 (2008)
  • Author(s): 中田光俊, 濱田潤一郎
  • Journal Title: 脳神経外科速報 18 Pages: 66-75
• [Journal Article] The guanine nucleotide exchange factors Trio, Ect2, and Vav3 mediate the invasive behavior of glioblastoma (2008)
  • Author(s): Salhia B, Tran NL, Chan A, Wolf A, Nakada M, et.al.
  • Journal Title: Am J Pathol 173 Pages: 1828-1838
  • Peer Reviewed
• [Journal Article] Role of myosin II activity and the regulation of myosin light chain phosphorylation in astrocytomas (2008)
  • Author(s): Salhia B, Hwang JH, Smith CA, Nakada M, et. al.
  • Journal Title: Cell Motility and the Cytoskeleton 65 Pages: 12-24
  • Peer Reviewed
• [Journal Article] Autotaxin induces glioma cell invasion, Autotaxin: a secreted autocrin e/paracrine factor that promotes glioma invasion. (2008)
  • Author(s): Hoelzinger DB, Nakada M, et. al.
  • Journal Title: Journal of Neuro-Oncology 86 Pages: 297-309
  • Peer Reviewed
• [Journal Article] Glioma cells on the run-the migratory transcriptome of 10 human glioma cell lines. (2008)
  • Author(s): Demuth T, Rennert JL, Hoelzin ger DB, Reavie LB, Nakada M, et. al.
  • Journal Title: BMC Genomics 9 Pages: 54-54
  • Peer Reviewed
• [Journal Article] Molecular targets of glioma invasion (2007)
  • Author(s): Nakada M, Nakada S, Demuth T, Tran NL, Hoelzinger DB, Berens ME.
  • Journal Title: Cell Mol Life Sci 64 Pages: 458-78
  • Peer Reviewed
• [Journal Article] Molecular targets of glioma invasion (2007)
  • Author(s): Nakada, Nakada S, et. al.
  • Journal Title: Cell Mol Life Sci 64 Pages: 458-478
  • Peer Reviewed
• [Presentation] グリオーマ内在幹細胞の浸潤関連遺伝子の探索 (2008)
  • Author(s): 中田光俊, 南部絵美, 吉田優也, 喜多大輔, 林康彦, 内山尚之, 林裕, 濱田潤一郎
  • Organizer: 第26回日本脳腫瘍学会
  • Place of Presentation: 松山
• [Presentation] Sphingosine-1-phosphate1 receptor regulates glioma cell proliferation and predicts patient survival (2008)
  • Author(s): 中田光俊、吉田優也、杉本直俊、林康彦、喜多大輔、内山尚之、林裕、濱田潤一郎
  • Organizer: 第67回日本癌学会学術総会
  • Place of Presentation: 名古屋
• [Presentation] 神経膠芽腫のMIB-1 labeling indexに影響する細胞増殖関連分子の解析 (2008)
  • Author(s): 中田光俊、吉田優也、喜多大輔、林康彦、内山尚之、林裕、濱田潤一郎
  • Organizer: 第67回日本脳神経外科学会総会
  • Place of Presentation: 盛岡
• [Presentation] The significant relationship between MIB-1 labeling index and S1P1 expression correlates with survival in glioblastoma (2008)
  • Author(s): Nakada M, Yoshida Y, Harada T, Kita D, Hayashi Y, Uchiyama N, Hayashi Y, Hamada JI
  • Organizer: 8^<th> Congress of the European Association for NeuroOncology
  • Place of Presentation: Barcelona, Spain
• [Presentation] 神経膠芽腫浸潤におけるEphA/ephrin-Aシグナルの意義(シンポジウム) (2008)
  • Author(s): 中田光俊、喜多大輔、林康彦、内山尚之、林裕、濱田潤一郎
  • Organizer: 第9回日本分子脳神経外科学会
  • Place of Presentation: 京都
• [Presentation] The expression level of S1P1 is related to MIB-1 labeling index which predicts survival in glioblastoma. (2008)
  • Author(s): Nakada M, Yoshida Y, Harada T, Kita D, Hayashi Y, Uchiyama N, Hayashi Y, Hamada JI
  • Organizer: The 17^<th> International Conference on Brain Tumor Research & Therapy
  • Place of Presentation: Hakodate, Japan
• [Presentation] The expression level of S1P1 is related to MIB-1 labeling index which predicts survival in glioblastoma (2008)
  • Author(s): Nakada M, et.al.
  • Organizer: The 17^<th> International Conference on Brain Turnor Research & Therapy
  • Place of Presentation: Hakodate, Japan
• [Presentation] 神経膠芽腫浸潤におけるEphA/ephrin-Aシグナルの意義 (2008)
  • Author(s): 中田光俊, 他
  • Organizer: 第9回日本分子脳神経外科学会
  • Place of Presentation: 京都
• [Presentation] Sphingosine-l-phosphate^1 receptor regulates glioma cell proliferation and predicts patient survival (2008)
  • Author(s): 中田光俊, 他
  • Organizer: 第67回日本癌学会学術総会
  • Place of Presentation: 名古屋
• [Presentation] Microarrayによる悪性脳腫瘍浸潤関連分子の同定と機能解析 (2007)
  • Author(s): 中田光俊
  • Organizer: 第6回北陸ポストゲノム研究フォーラム
  • Place of Presentation: 金沢
  • Year and Date: 2007-06-28
• [Presentation] CDK5/p35と神経膠芽腫浸潤 (2007)
  • Author(s): 中田光俊, 喜多大輔、林康彦、内山尚之、林裕、Berens ME、Lee KY、濱田潤一郎
  • Organizer: 第25回日本脳腫瘍学会
  • Place of Presentation: 東京
• [Presentation] The interaction between matriptase and MT1-MMP in glioma invasion. (2007)
  • Author(s): Nakada M, Miyamori H, Kita D, Hayashi Y, Uchiyama N, Hayashi Y, Sato H, Hamada JI
  • Organizer: 5^<th> Meeting for the Asian Society for Neuro-Oncology
  • Place of Presentation: Istanbul, Turkey
• [Presentation] 神経膠芽腫浸潤におけるMatriptaseの役割 (2007)
  • Author(s): 中田光俊、宮森久志、喜多大輔、林康彦、内山尚之、林裕、佐藤博、濱田潤一郎
  • Organizer: 第66回日本脳神経外科学会総会
  • Place of Presentation: 東京
• [Presentation] Ephrin-B2 ligand tyrosine kinase drives glioma invasion and predicts survival (2007)
  • Author(s): 中田光俊, 濱田潤一郎、Berens ME
  • Organizer: 第66回日本癌学会学術総会
  • Place of Presentation: 横浜
• [Presentation] 神経膠芽腫浸潤における細胞外基質分解酵素の相互作用 (2007)
  • Author(s): 中田光俊、宮森久志、喜多大輔、林康彦、内山尚之、林裕、佐藤博、濱田潤一郎
  • Organizer: 第8回日本分子脳神経外科学会
  • Place of Presentation: 兵庫
• [Presentation] Gliomaの浸潤におけるEphrin-B分子の役割 (2007)
  • Author(s): 中田光俊, 濱田潤一郎、Berens ME
  • Organizer: 第23回日本脳腫瘍病理学会
  • Place of Presentation: 熊本
• [Presentation] The interaction between matriptase and MT1-MMP in glioma invasion (2007)
  • Author(s): Nakada M, et. al.
  • Organizer: 5th Meeting for the Asian Society for Neuro-Oncology
  • Place of Presentation: Istanbul, Turkey