Expression and functional analysis of Aurora kinases in primary intracranial CNS lymphoma

• Project/Area Number: 19790998
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Cerebral neurosurgery
• Research Institution: Kobe University
• Principal Investigator: MIZUKAWA Katsu Kobe University, 大学院・医学研究科, 医学研究員 (80403260)
• Project Period (FY): 2007 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥3,220,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥420,000); Fiscal Year 2008: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2007: ¥1,400,000 (Direct Cost: ¥1,400,000)
• Keywords: 癌 / 遺伝子 / ゲノム / 脳・神経 / malignant lymphoma / Aurora-A / central nervous system

Research Abstract

2000年以降の当施設で手術摘出した頭蓋内悪性リンパ腫30例の組織でのAurora発現を検討した所、ほぼ全ての組織でAurora-A,Aurora-Bキナーゼの発現亢進を認めた。また、Auroraキナーゼの発現は、特に分裂期の細胞およびMIB-1で染色される細胞での発現が多かった。摘出した悪性リンパ腫からの細胞株の樹立は難しく、現在も細胞株の樹立中である。また、Auroraキナーゼのノックダウンによる細胞死や細胞周期の解析も現在検討中であるが、Auroraキナーゼのノックダウンによりアポトーシスが増加する傾向が認められた。頭蓋内悪性リンパ腫におけるAurora-A,Aurora-B蛋白の高発現より、頭蓋内悪性リンパ腫の治療標的としてこれらの蛋白の阻害が奏功する可能性があると考えられた。

Research Products

• [Journal Article] Eiji Kohmura, Glioblastoma multiforme associated with Klinefelter syndrome (2009)
  • Author(s): Takashi Sasayama, Katsu Mizukawa, Yoshio Sakagami, Takashi Mizowaki, Kazuhiro Tanaka, Chiho Ohbayashi Kiyoshi Mori, Sohei Kitazawa
  • Journal Title: Case report and review of the literature Neurologia medico-chirurgica (in press)
• [Journal Article] Two metachronous tumors induced by radiation therapy (2008)
  • Author(s): Sasayama T, Nishihara M, Tanaka K, Mizukawa K, Ehara K, Kanomata N, Kohmura E.
  • Journal Title: J Neurooncol 88(3) Pages: 315-20
  • Peer Reviewed
• [Journal Article] Two metachronous tumors induced by radiation therapy (2008)
  • Author(s): Sasayama T, Nishihara M, Tanaka K, Mizukawa K, Ehara K, Kanomata N, Kohmura E.
  • Journal Title: Journal of Neurooncology 88(3) Pages: 315-320
  • Peer Reviewed
• [Journal Article] Specific mTOR inhibitor rapamycin enhances cytotoxicity induced by alkylating agent 1-(4-amino-2- methyl-5- pyrimidinyl)methyl-3-(2-chloroethyl)-3-nitrosourea (ACNU) in human U251 malignant glioma cells (2007)
  • Author(s): Tanaka K, Sasayama T, Mizukawa K, Kawamura A, Kondoh T, Hosoda K, Fujiwara T, Kohmura E.
  • Journal Title: J Neurooncol 84(3) Pages: 233-44
  • Peer Reviewed
• [Journal Article] Trans-4-lodo, 4'-boranyl-chalcone induces antitumor activity against malignant glioma cell lines in vitro and in vivo (2007)
  • Author(s): Sasayama T, Tanaka K, Mizukawa K, Kawamura A, Kondoh T, Hosoda K, Kohmura E.
  • Journal Title: J Neurooncol 85(2) Pages: 123-32
  • Peer Reviewed
• [Journal Article] Trans-4-lodo,4'-boranyl-chalcone induces antitumor activity against malignant glioma cell lines in vitro and in vivo. (2007)
  • Author(s): Sasayama T, Tanaka K, Mizukawa K, Kawamura A, Kondoh T, Hosoda K, Kohmura E
  • Journal Title: Journal of Neuro-Oncology 85 Pages: 123-132
  • Peer Reviewed
• [Journal Article] Specific mTOR inhibitor rapamycin enhances cytotoxicity induced by alkylating agent 1-(4-amino-2-methyl-5-pyrimidinyl)methyl-3-(2-chloroethyl)-3-nitrosourea(ACNU)in human U251 malignant glioma cells. (2007)
  • Author(s): Tanaka K, Sasayama T, Mizukawa K, Kawamura A, Kondoh T, Hosoda K, Fujiwara T, Kohmura E.
  • Journal Title: Journal of Neuro-Oncology 84 Pages: 233-244
  • Peer Reviewed