Basic Approach for clarification of etiology of diseases of growth plate
Project/Area Number |
19791018
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Research Category |
Grant-in-Aid for Young Scientists (B)
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Allocation Type | Single-year Grants |
Research Field |
Orthopaedic surgery
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Research Institution | Osaka City University |
Principal Investigator |
IMAI Yuuki Osaka City University, 大学院・医学研究科, 病院講師 (10423873)
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Project Period (FY) |
2007 – 2008
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Project Status |
Completed (Fiscal Year 2008)
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Budget Amount *help |
¥3,840,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥540,000)
Fiscal Year 2008: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Fiscal Year 2007: ¥1,500,000 (Direct Cost: ¥1,500,000)
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Keywords | 核内受容体 / 軟骨 / 大腿骨頭すべり症 / 遺伝子改変マウス |
Research Abstract |
骨軟骨組織における核内受容体高次機能解明を目的として、軟骨組織特異的核内受容体遺伝子欠損マウス作出するために、9型コラーゲン(Col9)遺伝子座にCreRecombinaseを挿入したCol9-Cre(CreERT2)ノックインマウス作出のためのTargeting Vectorを完成させたが、ES細胞に遺伝子導入した結果、遺伝子組み換え効率が非常に悪く、Southern blotting法による確認により、組み換え体のES細胞を得られなかった。そこで、従来のエレクトロポレーション法ならびにREDシステムによる遺伝子組み換えによるTargeting Vectorの作成から、Invitrogen社製、BP recombinaseおよびLR recombinaseを用いたTargeting Vector作成に切り替え、Targeting Vectorが完成し、すべての塩基配列を確認後、現在ES細胞への遺伝子導入により、組み換え体の作出を行っている段階であり、残念ながら報告時点で、Col9-Creノックインマウスの作出には至っていない。また、PPARγ floxマウスはバッククロスが進んでいる。
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Report
(3 results)
Research Products
(85 results)
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[Journal Article] Cathepsin K-dependent toll-like receptor 9 signaling revealed in experimental arthritis.2008
Author(s)
Asagiri M, Hirai T, Kunigami T, Kamano S, Gober HJ, Okamoto K, Nishikawa K, Latz E, Golenbock DT, Aoki K, Ohya K, Imai Y, Morishita Y, Miyazono K, Kato S, Saftig P, Takayanagi H.
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Journal Title
Science 319(5863)
Pages: 624-7
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[Journal Article] Cathepsin K-dependent toll-like receptor 9 signaling revealed in experimental arthritis.2008
Author(s)
Asagiri M, Hirai T, Kunigami T, Kamano S, Gober HJ, Okamoto K, Nishikawa K, Latz E, Golenbock DT, Aoki K, Ohya K, Imai Y, Morishita Y, Miyazono K, Kato S, Saftig P, Takayanagi H
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Journal Title
Science. 319
Pages: 624-627
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Peer Reviewed
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[Journal Article] Estrogen prevents bone loss via estrogen receptor alpha and induction of Fasligand in osteoclasts2007
Author(s)
Nakamura T*, Imai Y*, Matsumoto T, Sato S, Takeuchi K, Igarashi K, Harada Y, Azuma Y, Krust A, Yamamoto Y, Nishina H, Takeda S, Takayanagi H, Metzger D, Kanno J, Takaoka K, Martin TJ, Chambon P, Kato S. (*; These authors equally contributed to this work)
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Journal Title
Cell 130(5)
Pages: 811-23
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[Journal Article] Estrogen prevents bone loss via estrogen receptor alpha and induction of Fas ligand in osteoclasts2007
Author(s)
Nakamura T^*, Imai Y^*, Matsumoto T, Sato S, Takeuchi K, Igarashi K, Harada Y, Azuma Y Krust A, Yamamoto Y Nishina H, Takeda S, Takayanagi H, Metzger D, Kanno J, Takaoka K, Martin TJ, Chambon P, Kato S.(*;These authors contributed equally to this work.)
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Journal Title
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Peer Reviewed
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[Presentation] Sex Steroids Hormone Mediate Osteoprotecitve Effects by Controlling Osteoclast Life Cycle2008
Author(s)
Yuuki Imai, Takashi Nakamura, Takahiro Matsumoto, Shingo Sato, Shu Takeda, Katsuhide Igarashi, Yoko Yamamoto, Jun Kanno Kunio Takaoka, T.John Mart in, Pierre Chambon, Shigeaki Kato
Organizer
2008 IBMS Davos WOrkshop
Place of Presentation
Davos
Year and Date
2008-03-11
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[Presentation] International Bone and Mineral Society Davos Workshop Sex Steroids Hormone Mediate Osteoprotecitve Effects by Controlling Osteoclast Life Cycle2008
Author(s)
Imai Y, Nakamura T, Matsumoto T, Sato S, Takeda S, Igarashi K, Yamamoto Y, Kanno J, Takaoka K, Martin TJ, Chambon P, Kato S
Organizer
Davos
Place of Presentation
Swiss
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[Presentation] ASBMR Sex Steroids Hormone Receptors in Osteoclasts Mediate Osteoprotecitve Effects by Regulating Its Life Cycle2007
Author(s)
22Yuuki Imai, Takashi Nakamura, Takahiro Matsumoto, Shu Takeda, Katsuhide Igarashi, Toru Fukuda, Yoko Yamamoto, Jun Kanno, Kunio Takaoka, Pierre Chambon, Shigeaki Kato
Place of Presentation
Honolulu, USA,
Year and Date
2007-09-19
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[Presentation] Sex Steroids Hormone Receptors in Osteoclasts Mediate Osteoprotecitve Effects by Regulating Its Life Cycle2007
Author(s)
Yuuki Imai, Takashi Nakamura, Takahiro Matsumoto, Shu Takeda, Katsuhide Igarashi, Toru Fukuda, Yoko Yamamoto, Jun Kanno, Kunio Takaoka, Pierre Chambon, Shigeaki Kato
Organizer
29th ASBMR
Place of Presentation
Honolulu
Year and Date
2007-09-19
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[Book] 栄養学研究の最前線2008
Author(s)
加藤茂明, 高田伊知郎, 中村貴, 山本陽子, 今井祐記
Total Pages
203
Publisher
建帛社
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