Role of heparan sulfate proteoglycan in joint development

• Project/Area Number: 19791036
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Orthopaedic surgery
• Research Institution: Kyushu University
• Principal Investigator: MATSUMOTO Yoshihiro Kyushu University, 医学研究院・整形外科, 助教 (10346794)
• Project Period (FY): 2007 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥3,600,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥600,000); Fiscal Year 2010: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000); Fiscal Year 2009: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2008: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2007: ¥1,000,000 (Direct Cost: ¥1,000,000)
• Keywords: ヘパラン硫酸 / 関節形成 / Bone morphogenetic protein / ノックアウトマウス / 関節発生 / EXT1 / Ext1 / 肢芽 / 前軟骨性凝集

Research Abstract

To elucidate the pathogenesis of joint diseases, the roles of heparan sulfate (HS) in the joint development was investigated. Loss of HS in the cell surface causd the diffuse distribution of bone morphogenetic protein and the abnormal formation of mesenchymal condensation that was critical for the joint development. These results clearly indicated the essential and cell biological roles of HS in the joint development.

Research Products

• [Journal Article] Roles of heparan sulfate in mammalian brain development current views based on the findings from Ext1 conditional knockout studies. (2010)
  • Author(s): Yamaguchi Y, Inatani M, Matsumoto Y, Ogawa J, Irie F.
  • Journal Title: Prog Mol Biol Transl Sci. 93 Pages: 133-152
• [Journal Article] Conditional ablation of the heparan sulfate-synthesizing enzyme Extlleads to dysregulation of bone morphogenic protein signaling and severe skeletal defects. (2010)
  • Author(s): Matsumoto Y., et al.
  • Journal Title: J Biol Chem. Volume: 285 Pages: 19227-19234
  • Peer Reviewed
• [Journal Article] Role of the VEGF-Flt-1-FAK pathway in the pathogenesis of osteoclastic bone destruction of giant cell tumors of bone (2010)
  • Author(s): Matsumoto Y., et al.
  • Journal Title: Journal of Orthopaedic Surgery and Research Volume: 5 Pages: 85-85
  • Peer Reviewed
• [Journal Article] Basic fibroblast growth factor in the bone microenvironment enhances cell motility and invasion of Ewing's sarcoma family of tumours by activating the FGFR1-P13K-Racl pathway (2010)
  • Author(s): Kamura S., Matsumoto Y., et al
  • Journal Title: Br J Cancer Volume: 103 Pages: 370-381
  • Peer Reviewed
• [Journal Article] Does ossification of the posterior longitudinal ligament affect the neurological outcome after traumatic cervical cord injury (2009)
  • Author(s): Okada S, Maeda T, Ohkawa Y, Harimaya K, Saiwai H, Kumamaru H, Matsumoto Y, Doi T, Ueta T, Shiba K, Iwamoto Y.
  • Journal Title: Spine 34 Pages: 1148-1152
  • NAID: 10027242692
  • Peer Reviewed
• [Journal Article] Heparan sulfate deficiency leads to Peters anomaly in mice by disturbing neural crest TGF-beta2 signaling. (2009)
  • Author(s): Iwao K, Inatani M, Matsumoto Y, Ogata-Iwao M, Takihara Y, Irie F, Yamaguchi Y, Okinami S, Tanihara H.
  • Journal Title: J Clin Invest. 119 Pages: 1997-2008
  • Peer Reviewed
• [Journal Article] Outcome of osteoplastic laminotomy for excision of spinal cord tumors. (2009)
  • Author(s): Matsumoto Y, et al, 他3名、1番目
  • Journal Title: J Orthop Surg. 17 Pages: 275-279
  • Peer Reviewed
• [Journal Article] Does ossification of the posterior longitudinal ligament affect the neurological outcome after traumatic cervical cord injury (2009)
  • Author(s): Okada S, Maeda T, Ohkawa Y, Harimaya K, Saiwai H, Kumamaru H, Matsumoto Y, Doi T, Ueta T, Shiba K, Iwamoto Y.
  • Journal Title: Spine. 34 Pages: 1148-1152
  • NAID: 10027242692
  • Peer Reviewed
• [Journal Article] Heparan sulfate deficiency leads to Peters anomaly in mice by disturbing neural crest TGF-beta2 signaling. (2009)
  • Author(s): Iwao K, Inatani M, Matsumoto Y, Ogata-Iwao M, Takihara Y, Irie F, Yamaguchi Y, Okinami S, Tanihara H
  • Journal Title: J Clin Invest. 119 Pages: 1997-2008
  • Peer Reviewed
• [Journal Article] Heparan sulfate deficiency leads to Peters anomaly in mice by disturbing neural crest TGF-beta2 signaling (2009)
  • Author(s): Iwao K, Inatani M, Matsumoto Y, Ogata-Iwao M, Takihara Y, Irie F, Yamaguchi Y, Okinami S, Tanihara H.
  • Journal Title: Journal of Clinical Investigation 119 Pages: 1997-2008
  • Peer Reviewed
• [Journal Article] Outcome of osteoplastic laminotomy for excision of spinal cord tumors (2009)
  • Author(s): Matsumoto Y, Harimaya K, Doi T, Maeda T, Iwamoto Y
  • Journal Title: Journal of Orthopaedic Surgery 17 Pages: 275-279
  • Peer Reviewed
• [Journal Article] Conditional ablation of the heparan sulfate-synthesizing enzyme Ext1 leads to dysregulation of bone morphogenic protein signaling and severe skeletal defects. (2008)
  • Author(s): Matsumoto Y, Matsumoto K, Irie F, Fukushi J, Stallcup WB, Yamaguchi Y.
  • Journal Title: J Biol Chem. 285(25) Pages: 19227-19234
  • Peer Reviewed
• [Journal Article] NG2 proteoglycan expression in mouse skin : altered postnatal skin de velopment in the NG2 null mouse (2008)
  • Author(s): Kadoya K, Fukushi J, Matsumoto Y, Yamaguchi Y, Stallcup WB
  • Journal Title: J Histochem Cytochem. 56 Pages: 295-303
  • Peer Reviewed
• [Journal Article] Intra-articular osteochondroma of the knee joint in a patient with hereditary multiple osteochondromatosis. (2007)
  • Author(s): Matsumoto Y, Matsuda S, Matono K, Oda Y, Tsuneyoshi M, Iwamoto Y.
  • Journal Title: Fukuoka Igaku Zasshi. 98 Pages: 425-430
  • Peer Reviewed
• [Journal Article] Netrin-1/DCC signaling in commissural axon guidance requires cell-autonomous expression of heparan sulfate. (2007)
  • Author(s): Matsumoto Y, Irie F, Inatani M, Tessier-Lavigne M, Yamaguchi Y.
  • Journal Title: J Neurosci. 27 Pages: 4342-4350
  • Peer Reviewed
• [Journal Article] NG2 Proteoglycan Expression in Mouse Skin : Altered Postnatal Skin Development in the NG2 Null Mouse. (2007)
  • Author(s): Kadoya K, Fukushi JI, Matsumoto Y, Yamaguchi Y, Stallcup WB.
  • Journal Title: J Histochem Cytochem. 56 Pages: 295-303
  • Peer Reviewed
• [Journal Article] Intra-articular osteochondroma of the knee joint in a patient with hereditary multiple osteochondromatosis. (2007)
  • Author(s): Matsumoto Y, Matsuda S, Matono K, Oda Y, Tsuneyoshi M, Iwamoto Y
  • Journal Title: Fukuoka Igaku Zasshi. 98 Pages: 425-430
  • Peer Reviewed
• [Journal Article] Netrin-1/DCC signaling in commissural axon guidance requires cell-autonomous expression of heparan sulfate. (2007)
  • Author(s): Matsumoto Y, Irie F, Inatani M, Tessier-Lavigne M, Yamaguchi Y
  • Journal Title: J Neurosci 27 Pages: 4342-4350
  • Peer Reviewed
• [Journal Article] NG2 Proteoglycan Expression in Mouse Skin: Altered Postnatal Skin Development in the NG2 Null Mouse. (2007)
  • Author(s): Kadoya K, Fukushi JI, Matsumoto Y, Yamaguchi Y, Stallcup WB
  • Journal Title: J Histochem Cytochem 56 Pages: 295-303
  • Peer Reviewed
• [Journal Article] Netrin-1/DCC signaling in commissural axon guidance requires cell-autonomous expression of heparan sulfate (2007)
  • Author(s): Yoshihiro, Matsumoto, et. al.
  • Journal Title: Journal of Neurosicience 27 Pages: 4342-4350
  • Peer Reviewed
• [Journal Article] Intra-articular osteochondroma of the knee joint in a patient with hereditary multiple osteochondromatosis (2007)
  • Author(s): Yoshihiro, Matsumoto, et. al.
  • Journal Title: Fukuoka Acta Medica 98 Pages: 425-530
  • Peer Reviewed
• [Presentation] 多発性遺伝性骨軟骨腫症の病因および二次性軟骨肉腫の病態と治療 (2010)
  • Author(s): 松本嘉寛
  • Organizer: 第22回日本整形外科学会骨系統疾患研究会
  • Place of Presentation: 徳島
  • Year and Date: 2010-11-27
• [Presentation] Conditional ablation of the heparan sulfate-synthesizing enzyme Ext1 leads to dysregulation of bone morphogenic protien signaling and sever skeletal defects (2010)
  • Author(s): Yoshihiro Matsumoto, Kazu Matsumoto, Yu Yamaguchi, Fumitosi Irie, Yukihide Iwamoto
  • Organizer: The 32nd Annual Meeting of American Society for Bone and Mineral Research
  • Place of Presentation: Toronto, Canada
  • Year and Date: 2010-10-15
• [Presentation] Y Basic Fibroblast Growth Factor enhances invasive and metastatic phenotype of Ewing sarcoma cells by stimulating the cell migration (2010)
  • Author(s): Kamura, S ; Matsumoto, Y ; Fukushi, J ; Fujiwara, T ; Okada, Y ; Iida, K ; Matsuda, S ; Harimaya, K ; Yoshida, T ; Matsunobu, T ; Iwamoto
  • Organizer: The 56th Annual Meeting of the Orthopaedic Research Society
  • Place of Presentation: New Orleans, USA
  • Year and Date: 2010-02-20
• [Presentation] 末梢神経発生におけるヘパラン硫酸プロテオグリカンの役割 (2008)
  • Author(s): 松本嘉寛
  • Organizer: 日本結合組織学会学術大会
  • Place of Presentation: 東京
  • Year and Date: 2008-05-29
• [Presentation] Essential role of heparan sulfate on axon guidance in developmental spinal cord (2007)
  • Author(s): Y Matsumoto, et al.
  • Organizer: The 53th annual meeting of the Orthopaedic Research Society
  • Place of Presentation: San Diego,USA
  • Year and Date: 2007-02-11
• [Presentation] Essential role of heparan sulfate on axon guidance in developmental spinal cord (2007)
  • Author(s): Yoshihiro Matsumoto, et al.
  • Organizer: The 53th annual meeting of the Orthopaedic Research Society
  • Place of Presentation: San Diego, USA
  • Year and Date: 2007-02-11
• [Presentation]
  • Organizer: 日本結合織学会
  • Place of Presentation: 東京
• [Remarks] 平成21年度日本対がん協会福岡県支部がん研究助成金ホームページ等
• [Remarks] 平成21年度日本対がん協会福岡県支部がん研究助成金