Molecular biological analysis of myocardial preservation via redox regulation of Akt

• Project/Area Number: 19791069
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Anesthesiology/Resuscitation studies
• Research Institution: Nagasaki University
• Principal Investigator: MURATA Hiroaki Nagasaki University, 大学病院, 助教 (90437856)
• Project Period (FY): 2007 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥3,650,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥450,000); Fiscal Year 2008: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000); Fiscal Year 2007: ¥1,700,000 (Direct Cost: ¥1,700,000)
• Keywords: Akt / レドックス / 酸化ストレス / 心筋保護 / glutaredoxin

Research Abstract

Aktは酸化ストレスにより一過性にリン酸化されると同時に、活性部位近傍に存在する還元型のcysteine297とcysteine311は酸化されS-S結合を形成する。変異解析の結果よりAkt分子はcysteine297およびcysteine311のいずれもが還元型として存在している状態でのみ、酸化ストレスに反応してリン酸化され、活性化されることを明らかにした。

Research Products

• [Presentation] Redox regulation of Akt is crucial for its cardioprotective effect. (2010)
  • Author(s): Hiroaki Murata, Chiaki Inadomi, Koji Sumikawa
  • Organizer: International Anesthesia research Society 2010 Annual Meeting
  • Place of Presentation: Honolulu
  • Year and Date: 2010-03-21
• [Presentation] Cardioprotective effects of glutaredoxin 1 induced by suppressing S-nitrosylation of GAPDH. (2008)
  • Author(s): Chiaki Inadomi, Yoshito Ihara, Hiroaki Murata, Koji Sumikawa, Takahito Kondo
  • Organizer: American Society of Anesthesiologists 2008 Annual meeting
  • Place of Presentation: Orlando
  • Year and Date: 2008-10-19
• [Presentation] Glutaredoxin protects nitric oxide-induced apoptosis and suppresses the oxidative modification of GAPDH. (2007)
  • Author(s): 稲冨千亜紀, 井原義人, 村田寛明, 澄川耕二, 近藤宇史
  • Organizer: 第80回日本生化学会大会
  • Place of Presentation: 横浜
  • Year and Date: 2007-12-14
• [Presentation] Glutaredoxin protects nitric oxide-induced apoptosis and suppresses the oxidative modification of GAPDH (2007)
  • Author(s): 稲冨千亜紀
  • Organizer: 日本生化学会
  • Place of Presentation: 横浜
  • Year and Date: 2007-12-14