Development of a new procedure for measurement of brain water content -aquaporin as a target of a new therapy for brain edema-

• Project/Area Number: 19791078
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Anesthesiology/Resuscitation studies
• Research Institution: Nagoya City University
• Principal Investigator: HIRATE Hiroyuki Nagoya City University, 大学院・医学研究科, 助教 (20363939)
• Project Period (FY): 2007 – 2009
• Project Status: Completed (Fiscal Year 2009)
• Budget Amount: ¥3,770,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥570,000); Fiscal Year 2009: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2008: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2007: ¥1,300,000 (Direct Cost: ¥1,300,000)
• Keywords: 蛋白質 / 脳・神経 / 脳神経疾患 / アクアポリン4 / マニトール / 脳浮腫 / 脳水分測定

Research Abstract

Brain edema leading to an expansion of brain volume has a crucial impact on morbidity and mortality following brain damages. It is necessary to develop a new procedure for measurement of brain water content for experiments. Here, I have developed a new method of measurement of focal brain water content by Karl-Fischer titration. The method will be useful for studies of brain edema.

Research Products

• [Journal Article] Lactic acid increases aquaporin 4 expression on the cell membrane of cultured rat astrocytes. (2008)
  • Author(s): Morisima M, Aoyama M, Iida Y, Yamamoto N, Hirate Hiroyuki, Arima H, Fujita Y, Sasano H, Tsuda T, Katsuya H, Asai K, Sobue K
  • Journal Title: Neuroscience Research 61(1) Pages: 18-26
  • Peer Reviewed
• [Journal Article] 脳浮腫の発症機序と水チャネル「アクアポリン」の機能 (2008)
  • Author(s): 祖父江和哉、平手博之、飯田裕子、高柳猛彦
  • Journal Title: Anesthesia 21Century 10 Pages: 1835-1839
  • Peer Reviewed
• [Presentation] 硬膜外ブロック後に-過性硬膜外血腫を生じた高度脊椎変形を持つ一症例 (2010)
  • Author(s): 平手博之、蓑輪暁久
  • Organizer: 第22回日本老年麻酔学会
  • Place of Presentation: 横浜シンポジア (横浜)
  • Year and Date: 2010-01-23
• [Presentation] ラット敗血症モデルの副腎におけるアクアポリン(AQP)の発現 (2009)
  • Author(s): 高柳猛彦、平手博之、藤田義人、松本麗、祖父江和哉、浅井清文
  • Organizer: 日本麻酔科学会第56回学術集会
  • Place of Presentation: 神戸
  • Year and Date: 2009-08-17
• [Presentation] マン二トールによる水チャネルaquaporin4を介した脳水分量調節機構 (2009)
  • Author(s): 平手博之、有馬一、高柳猛彦、飯田裕子、伊藤弘晃、成松紀子、藤田義人、薊隆文、浅井清文、祖父江和哉
  • Organizer: 36回日本集中治療医学会学術集会
  • Place of Presentation: 大阪
  • Year and Date: 2009-02-26
• [Presentation] マンニトールによる水チャネルaquaporin4を介した脳水分量調節機構 (2009)
  • Author(s): 平手博之
  • Organizer: 第36回日本集中治療医学会学術集会
  • Place of Presentation: 大阪国際会議場
  • Year and Date: 2009-02-26
• [Remarks] 名古屋市立大学大学院医学研究科麻酔・危機管理医学分野ホームページ
  • URL: http://www.ncu-masui.jp