Influence on prostate cancer of bone marrow stem cell (pre-adipocytes) :Comprehensive analysis of the signaling pathways

• Project/Area Number: 19791115
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Urology
• Research Institution: Saga University
• Principal Investigator: SATOH Yuji Saga University, 医学部, 講師 (40363436)
• Project Period (FY): 2007 – 2009
• Project Status: Completed (Fiscal Year 2009)
• Budget Amount: ¥3,640,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥540,000); Fiscal Year 2009: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2008: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2007: ¥1,300,000 (Direct Cost: ¥1,300,000)
• Keywords: 前立腺癌 / 脂肪細胞 / 3次元培養 / シグナル伝達 / 免疫染色 / Western Blot法 / Real-Time RT PCR / cDNA microarray

Research Abstract

We examined signaling pathways with human prostate cancer cell line and pre-adipocytes .Cancer cells were co-cultured with the isolated mature adipocytes in 3-D collagen gel matrix culture. In LNCaP, humoral factors and cell to cell contaction are necessary for proliferation. In PC-3, cell to cell contaction is necessary for differentiation. In DU145, humoral factors and cell to cell contaction are necessary for proliferation and differentiation. As a result of cDNA microarray, Prostate-specific expression PSGR2 and integrin significantly increased.

Research Products

• [Journal Article] Effects of adipocytes on the proliferation and differentiation of prostate cancer cells in a 3-D culture model (2010)
  • Author(s): Arata Kaneko, Yuji Satoh, Yuji Tokuda, Chisato Fujiyama, Kazuma Udo, Jiro Uozumi
  • Journal Title: International Journal of Urology Vol.17、No.4 Pages: 369-376
  • Peer Reviewed
• [Journal Article] Effects of adipocytes on the proliferation and differentiation of prostate cancer cells in a 3-D culture model (2010)
  • Author(s): Arata Kaneko, Yuji Satoh, Yuji Tokuda, Chidato Fujiyama, Kazuma Udo Jiro Uozumi
  • Journal Title: International Journal of Urology 17 Pages: 369-376
  • Peer Reviewed
• [Journal Article] コラーゲンゲル三次元混合培養による前立腺癌細胞に対する脂肪細胞の影響の検討 (2007)
  • Author(s): 金子新, 佐藤勇司, 藤山千里, 徳田雄治, 有働和馬, 魚住二郎
  • Journal Title: 西日泌尿器科 Vol.69 Pages: 171-175
  • NAID: 10020621921
• [Journal Article] コラーゲンゲル三次元混合培養による前立腺癌細胞に対する脂肪細胞の影響の検討. (2007)
  • Author(s): 金子新, 佐藤勇司, 藤山千里, 徳田雄治, 有働和馬, 魚住二郎
  • Journal Title: 西日泌尿器科 69 Pages: 171-175
  • NAID: 10020621921
• [Presentation] 脂肪細胞が前立腺癌細胞に与える影響について;Prostate cancer cell-adipocyte interaction in vitro (2008)
  • Author(s): 佐藤勇司, 金子新, 吉永北斗, 有働和馬, 徳田雄治, 藤山千里, 魚住二郎
  • Organizer: 第17回泌尿器分子・細胞研究会
  • Place of Presentation: 東京
  • Year and Date: 2008-02-06