Changes and functions of pre-ovulatory human granulosa cells.

• Project/Area Number: 19791136
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Obstetrics and gynecology
• Research Institution: Gunma University
• Principal Investigator: KISHI Hiroshi Gunma University, 医学部, 講師 (10375545)
• Project Period (FY): 2007 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥3,920,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥720,000); Fiscal Year 2010: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2009: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2008: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2007: ¥800,000 (Direct Cost: ¥800,000)
• Keywords: 生殖生理 / シグナル伝達 / 発現制御 / 体外受精胚移植 / 顆粒膜細胞 / mRNA

Research Abstract

With pre-ovulatory human granulosa cells, which are obtained on the occasion of ICSI therapy for infertile women and usually disposed, we analyzed the patterns of gene expression using quantitative PCR method.
The amount of the cells obtained with this therapy is extremely small. Nevertheless, we succeeded in quantifying the expression of one gene. For other genes, we could not get enough result.