Digital Karyotyping in ovarian clear cell carcinoma

• Project/Area Number: 19791152
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Obstetrics and gynecology
• Research Institution: Shimane University
• Principal Investigator: NAKAYAMA Kentaro Shimane University, 医学部, 講師 (70346401)
• Project Period (FY): 2007 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥3,540,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥240,000); Fiscal Year 2008: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2007: ¥2,500,000 (Direct Cost: ¥2,500,000)
• Keywords: 癌 / ゲノム / 卵巣癌 / 分子標的

Research Abstract

Disital Karyotyping (DK)、Serial Analysis Gene Expression(SAGE)を用いて網羅的な遺伝子発現解析を行い、卵巣癌特有の遺伝子変化を同定し、卵巣癌の治療を目指した分子標的を同定する事を目的とした。近年、造血器腫瘍を中心に癌遺伝子の機能がクローズアップされているBTB/POZ遺伝子群に注目した。BTB/POZ遺伝子群の中でNAC-1遺伝子が有意に高発現している事を発見した。NAC-1は再発卵巣癌でタンパク、遺伝子発現共に有意に高かった。また、in vitoro, in vivoの両方でNAC-1はOncogenicな機能を有する事を発見した。

Research Products

• [Journal Article] Expression of a BTB/POZ protein, NAC1, is essential for the proliferation of normal cyclic endometrial glandular cells and is up-regulated by estrogen. (2009)
  • Author(s): Ishibashi M, Nakayama K, Yeasmin S, Katagiri A, Iida K, Nakayama N, Miyazaki K.
  • Journal Title: Clin Cancer Res. 15(3) Pages: 804-809
• [Journal Article] Fractional allelic loss as a potential biomarker of risk prediction in early-stage mucinous ovarian tumors of low malignant potential. (2009)
  • Author(s): Nakayama K, Nakayama N, Ishibashi M, Yeasmin S, Fukumoto M, Miyazaki K
  • Journal Title: European Journal of Gynecological Oncology (in press)
• [Journal Article] Primary osteosarcoma of ovary. (2009)
  • Author(s): Yeasmin S, Nakayama K, Ishibashi M, Katagiri A, Iida K, Manabe A, Nabika T, Nakayama N, Miyazaki K
  • Journal Title: International Journal of Clinical Oncology (in press)
  • NAID: 10025147960
• [Journal Article] NAC-1, a potential stem cell pluripotency factor, contributes to paclitaxel resistance in ovarian cancer through inactivating Gadd45 pathway (2009)
  • Author(s): Jinawath N, Vasoontara C, Yap KL, Thiaville MM, Nakavama K, Wang TL, Shih IM.
  • Journal Title: Oncogene (Mar 23. [Epub ahead of print])
  • Peer Reviewed
• [Journal Article] BTB/POZ protein, NAC1, is essential for the proliferation of normal cyclic endometrial glandular cells and is up-regulated by estrogen (2009)
  • Author(s): Ishibashi M, Nakayama K, Yeasmin S, Katagiri A, Iida K, Nakayama N, Miyazaki K
  • Journal Title: Clin Cancer Res. 15 Pages: 804-809
  • Peer Reviewed
• [Journal Article] KRAS or BRAF mutation status is a useful predictor of sensitivity to MEK inhibition in ovarian cancer. (2008)
  • Author(s): Nakayama N, Nakayama K, Yeasmin S, Ishibashi M, Katagiri A, Iida K, Fukumoto M, Miyazaki K
  • Journal Title: Br J Cancer. 1699(12) Pages: 2020-8
• [Journal Article] A BTB/POZ protein, NAC-1, a tumor recurrence associated gene, as a potential target for taxol resistance in ovarian cancer. (2008)
  • Author(s): Ishibashi M, Nakayama K, Yeasmin S, Katagiri A, Iida K, Nakayama N, Fukumoto M, Miyazaki K
  • Journal Title: Clin Cancer Res. 14(10) Pages: 3149-55
• [Journal Article] Expression of the Bric-a-Brac Tramtrack Broad Complex Protein NAC-1 in Cervical Carcinomas Seems to Correlate with Poorer Prognosis. (2008)
  • Author(s): Yeasmin S, Nakayama K, Ishibashi M, Katagiri A, Iida K, Purwana IN, Nakayama N, Miyazaki K
  • Journal Title: Clin Cancer Res. 14(6) Pages: 1686-91
• [Journal Article] Genetic classification of ovarian carcinoma based on microsatellite analysis : relationship to clinicopathological features and patient survival. (2008)
  • Author(s): Huan Z, Nakayama K, Nakayama N, Ishibashi M, Yeasmin S, Katagiri A, Purwana IN, Iida K, Maruyama R, Fukumoto M, Miyazaki K
  • Journal Title: Oncol Rep. 19(3) Pages: 775-81
• [Journal Article] KRAS or BRAF mutation status is a useful predictor of sensitivity to MEK inhibition in ovarian cancer (2008)
  • Author(s): Nakayama N, Nakavama K, Yeasmin S, Ishibashi M, Katagiri A, Iida K, Fukumoto M, Miyazaki K.
  • Journal Title: Br J Cancer. 16 Pages: 2020-2028
  • Peer Reviewed
• [Journal Article] A BTB/POZ Protein NAC-1, a tumor recurrence associated gene, as a potential target for taxol resistance in ovarian cancer. (2008)
  • Author(s): Ishibashi M, Nakavama K, Yeasmin S, Katagiri A, Iida K, Nakayama N, Miyazaki K.
  • Journal Title: Clin Cancer Res. (in press)
  • Peer Reviewed
• [Journal Article] Expression of the Bric-a-Brac Tramtrack Broad Complex Protein NAC-1 in Cervical Carcinomas Seems to Correlate with Poorer Prognosis. (2008)
  • Author(s): Yeasmin S, Nakayama K. lshibashi M, Katagiri A, lida K, Purwana IN, Nakayania N, Miyazaki K.
  • Journal Title: Clin Cancer Res. 14 Pages: 1686-91
  • Peer Reviewed
• [Journal Article] Genetic classification of ovarian carcinoma based on microsatellite analysis: relationship to clinicopathological features and patient survival. (2008)
  • Author(s): Huan Z, Nakayama k, Nakayama N, Ishibashi M, Yeasmin S, Katagtri A, Purwana IN. lida K, Maruyama R, Fukumoto M, Mivazaki K.
  • Journal Title: Oncol Rep. 19 Pages: 775-81
  • Peer Reviewed
• [Presentation] 卵巣癌、子宮頸部腺癌における転写制御因子NAC-1をターゲットとした分子標的治療確立のための基礎的検討 (2009)
  • Author(s): 中山健太郎、石橋雅子,ヤスミン・シャミマ,片桐敦子,飯田幸司,中山真美,宮崎康二
  • Organizer: 第61回日本婦人科がん分子標的研究会,日本産婦人科学会総会
  • Place of Presentation: 京都
  • Year and Date: 2009-04-05
• [Presentation] 転写制御因子NAC-1をターゲットにした子宮頸部腺癌の分子標的治療の確立 (2008)
  • Author(s): 中山健太郎,シャミマヤスミン,石橋雅子,片桐敦子,飯田幸司,中山真美,宮崎康二,中山理,大月寛郎,小林寛
  • Organizer: 第45回特非日本婦人科腫瘍学会
  • Place of Presentation: 金沢
  • Year and Date: 2008-11-12
• [Presentation] 卵巣癌におけるSAGEを用いた新規分子標的の検索-転写制御因子NAC-1の分子標的としての可能性- (2008)
  • Author(s): 中山健太郎、石橋雅子,ヤスミン・シャミマ,片桐敦子,飯田幸司,中山真美,宮崎康二
  • Organizer: 第7回日本婦人科がん分子標的研究会
  • Place of Presentation: 名古屋
  • Year and Date: 2008-07-18
• [Presentation] 卵巣癌におけるクロマチンリモデリング因子RSF-1の遺伝子増幅 (2008)
  • Author(s): 中山健太郎、石橋雅子,ヤスミン・シャミマ,片桐敦子,飯田幸司,中山真美,宮崎康二
  • Organizer: 第49回日本臨床細胞学会
  • Place of Presentation: 東京
  • Year and Date: 2008-06-07
• [Presentation] 再発卵巣癌におけるSAGEを用いた新規分子標的の検索-転写制御因子NAC-1の分子標的としての可能性- (2008)
  • Author(s): Nakayama K, Ishibashi M, Katagiri A. Iida K, Yeasmin S,
  • Organizer: 日本産科婦人科学会総会
  • Place of Presentation: 東京
  • Year and Date: 2008-04-12
• [Presentation] 卵巣癌におけるSAGEを用いた新規分子標的の検索-転写制御因子NAC-1の分子標的としての可能性- (2008)
  • Author(s): 中山健太郎、石橋雅子,ヤスミン・シャミマ,片桐敦子,飯田幸司,中山真美,宮崎康二
  • Organizer: 第60回日本婦人科がん分子標的研究会,日本産婦人科学会総会
  • Place of Presentation: 横浜
  • Year and Date: 2008-04-11
• [Presentation] 卵巣癌における転写制御因子NAC-1をターゲットとした分子標的治療確立のための基礎的検討 (2008)
  • Author(s): Nakayama K, Ishibashi M, Katagiri A, Iida K, Yeasmin S, Nakayama N, Miyazaid K.
  • Organizer: 日本産科婦人科学会総会
  • Place of Presentation: 京都
  • Year and Date: 2008-04-05
• [Presentation] Sequence mutations and amplification of PIK3CA and AKT2 genes in purified ovarian serous neoplasms (2007)
  • Author(s): 中山健太郎,石橋雅子,ヤスミン・シャミマ,片桐敦子,飯田幸司,Tian-le Wang,le-ming Shin,宮崎康二
  • Organizer: 第66回日本癌学会学術総会
  • Place of Presentation: 横浜