Study on infertility by chromosomal aberration

• Project/Area Number: 19791159
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Obstetrics and gynecology
• Research Institution: Osaka City University
• Principal Investigator: YOSHIDA Kayo Osaka City University, 大学院・医学研究科, 准教授 (30311921)
• Project Period (FY): 2007 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥3,630,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥330,000); Fiscal Year 2008: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2007: ¥2,200,000 (Direct Cost: ¥2,200,000)
• Keywords: 遺伝子 / 細胞・組織 / 発現制御 / 発生・分化

Research Abstract

減数分裂期の染色体動態の解明は、不妊の原因究明や胎児の染色体異常において重要な課題である。我々は減数分裂の染色体分配に関与するシュゴシン遺伝子(Sgo1,Sgo2)のノックアウトマウスを作製したが、胚性致死であった。また、マウスにおいてSgo1, Sgo2遺伝子は胸腺・脾臓・小腸・子宮・卵巣・精巣などに発現し、両遺伝子は、細胞の増殖に関連していることが明らかとなった。さらに、その発現量はSgo2 遺伝子が精巣において極端に高いなど特徴が見られたことから、両遺伝子は異なる機能を果たし連携していると考えられた。

Research Products

• [Journal Article] Rad18 is required for long-term maintenance of spermatogenesis in mouse testes (2009)
  • Author(s): Sun J., Yomogida K., Sakao S., Yamamoto H., Yoshida K., Watanabe K., Morita T., Araki K., Yamamura K. and Tateishi S.
  • Journal Title: Mechnisms. Dev. 126 Pages: 173-183
  • Peer Reviewed
• [Journal Article] Rad18 is required for long-term maintenance of spermatogenesis in mouse testes (2009)
  • Author(s): Jinghua Sun, Kentaro Yomogida, Suzu Sakao, Haruna Yamamoto, Kayo Yoshida, Kenji Watanabe, Takashi Morita, Kimi Araki, Ken-ichi Yamamura and Satoshi Tateishi
  • Journal Title: Mechnisms. Dev 126 Pages: 173-183
  • Peer Reviewed
• [Journal Article] Unified mode of centromeric protein by shugoshin in mammalian oocyte and somatic cells. (2008)
  • Author(s): Lee J., Kitajima T., Tanno Y., Yoshida K., Morita T., Miyano T., Miyake M., Watanabe Y.
  • Journal Title: Nature cell biology. 10 Pages: 42-52
  • Peer Reviewed
• [Journal Article] Unified mode of centromeric centromeric protection by shugoshin in mammslian oocytes and somatic cells (2008)
  • Author(s): Lee J, Kitajima TS, Tanno Y, Yoshida K, Morita T, Miyano T, Miyake M, Watanabe Y
  • Journal Title: nature cell biology 10 Pages: 42-52
  • Peer Reviewed
• [Presentation] 宇宙放射線が哺乳動物の生殖細胞に及ぼす影響の研究 (2008)
  • Author(s): 吉田佳世、吉田周平、森田隆
  • Organizer: 日本宇宙生物科学会第22回大会
  • Place of Presentation: 奈良県立医科大学
  • Year and Date: 2008-09-26
• [Presentation] Effects of X-radiation to development of mammalian embryos. (2008)
  • Author(s): Yoshida K., Yoshida S., Morita T.
  • Organizer: 19th Annual NASA Space Radiation Investigators' Workshop
  • Place of Presentation: 米国・フィラデルフィア
  • Year and Date: 2008-07-01
• [Presentation] Effects of X-radiation to development of mammalian embryos. (2008)
  • Author(s): Yoshida, K., Yoshida, S., Morita, T.
  • Organizer: 19^<th> Annual NASA Space Radiation Investigators' Workshop
  • Place of Presentation: 米国・フィラデルフィア
  • Year and Date: 2008-07-01