Development of noninvasive and real-time assessment system for the novel human endometriosis model mouse

• Project/Area Number: 19791162
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Obstetrics and gynecology
• Research Institution: Keio University
• Principal Investigator: MASUDA Hirotaka Keio University, 医学部, 助教 (80317198)
• Project Period (FY): 2007 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥3,720,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥420,000); Fiscal Year 2008: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2007: ¥1,900,000 (Direct Cost: ¥1,900,000)
• Keywords: 子宮内膜 / 子宮内膜症 / モデル / BLI(bioluminescence imaging) / エストロゲン / プロゲステロン / 免疫不全マウス / モデルマウス / リアルタイム / BLI(bioluminescence imaging )

Research Abstract

我々は、少量の子宮内膜細胞を重度免疫不全マウスの腎被膜下へ移植することで均一の異所性内膜様組織を持つモデルを作成し、その内膜様組織の非侵襲的かつリアルタイムな定量化が長期にわたり体外より可能なシステムを開発した。このシステムは既存の内膜症動物モデルが持つ問題点をすべて克服しており、内膜症および正常内膜の機能メカニズムの解明ツールとしてだけでなく内膜症の新規薬剤の評価システムとしても有用であると考えられる。

Research Products

• [Journal Article] In vivo imaging in humanized mice (2008)
  • Author(s): Masuda H, Okano HJ, Maruyama T, Yoshimura Y, Okano H, Matsuzaki Y
  • Journal Title: Curr Top Microbiol Immunol 324 Pages: 179-196
  • Peer Reviewed
• [Journal Article] In vivo imaging in humanized mice (2008)
  • Author(s): Hirotaka Masuda
  • Journal Title: Current Topics in Microbiology and Immunology 324 Pages: 179-196
• [Journal Article] Noninvasive and real-time assessment of reconstructed functional human endometrium in NOD/SCID/{gamma}cnull immunodeficient mice (2007)
  • Author(s): Masuda H, Maruyama T, Hiratsu E, Yamane J, Iwanami A, Nagashima T, Ono M, Miyoshi H, Okano HJ, Ito M, Tamaoki N, Nomura T, Okano H, Matsuzaki Y, Yoshimura Y
  • Journal Title: Proc Natl Acad Sci U S A 104(6)
• [Journal Article] 子宮内膜幹細胞 (2007)
  • Author(s): 升田博隆,丸山哲夫,岡野栄之,松崎有未,吉村泰典
  • Journal Title: 産婦人科の世界 59(3) Pages: 33-42
• [Journal Article] Noninvasive and real-time assessment of reconstructed functional human endometrium in NOD/SCID/{gamma}cnull immunodeficient mice. (2007)
  • Author(s): Hirotaka Masuda
  • Journal Title: Proceedings of the National Academy of Sciences of theUnited States of America 104 Pages: 1925-1930
  • Peer Reviewed
• [Presentation] ヒト子宮内膜幹細胞様細胞の同定・分離とその幹細胞特性の機能解析 (2008)
  • Author(s): 升田博隆・丸山哲夫・小野政徳・長島隆・梶谷宇・内田浩・浅田弘法・岡野栄之・松崎有未・吉村泰典
  • Organizer: 第13回日本生殖内分泌学会学術集会
  • Place of Presentation: 大阪国際会議場・大阪市
  • Year and Date: 2008-11-29
• [Presentation] ヒト子宮内膜幹細胞様細胞の同定・分離とその幹細胞特性の機能解析 (2008)
  • Author(s): Hirotaka Masuda
  • Organizer: 第13回日本生殖内分泌学会学術集会
  • Place of Presentation: 大阪国際会議場, 大阪市
  • Year and Date: 2008-11-29
• [Presentation] Isolation and functional analysis of putative human endometrial stem/progenitor cells (2008)
  • Author(s): H. Masuda, T. Maruyama, M. Ono, T. Nagashima, T. Kajitani, H. Okano, Y. Matsuzaki, Y. Yoshimura
  • Organizer: ESHRE2008(24th Annual Meeting of The Europian Society of Human Reproduction andEmbryology)
  • Place of Presentation: Barcelona, Spain
  • Year and Date: 2008-07-07
• [Presentation] Isolation and functional analysis of putative human endometria 1 stem/progenitor cells (2008)
  • Author(s): Hirotaka Masuda
  • Organizer: ESHRE 2008 (24rd Annual Meeting of The Europian Society of Human Reproduction and Embryology)
  • Place of Presentation: Barcelona, Spain
  • Year and Date: 2008-07-07
• [Presentation] ヒト子宮内膜幹細胞の同定・分離の試み (2008)
  • Author(s): 升田博隆・丸山哲夫・小野政徳・岡野栄之・松崎有未・吉村泰典
  • Organizer: 第49回日本臨床細胞学会総会
  • Place of Presentation: グランドプリンスホテル新高輪・東京ワークショップ(再生医療,最近のトピックス)
  • Year and Date: 2008-06-08
• [Presentation] 新規子宮内膜症モデルマウスの作成とそのリアルタイム解析システムの開発 (2007)
  • Author(s): 升田博隆・丸山哲夫・吉村泰典・岡野栄之・松崎有未
  • Organizer: 第28回日本再生・炎症医学会
  • Place of Presentation: (京王プラザホテル・東京)ワークショップ(分子イメージング)
  • Year and Date: 2007-08-03
• [Presentation] Reconstruction and in vivo bioluminescence imaging of human endometrium in immunodeficient mice : its potential application as an endometriosis model (2007)
  • Author(s): H. Masuda, T. Maruyama, T. Nagashima, M. Ono, T. Kajitani, H. Uchida, K. Ohta, T. Arase, M. Kagami, H. Asada, H. Okano, Y. Matsuzaki, Y. Yoshimura
  • Organizer: ESHRE 2007 (23rd Annual Meeting of The Europian Society of Human Reproduction and Embryology)
  • Place of Presentation: Lyon, France
  • Year and Date: 2007-07-03
• [Presentation] Reconstruction and in vivo bioluminescence imaging of human endometrium in immunodeficient mice: its potential application as an endometiiosis model. (2007)
  • Author(s): Hirotaka Masuda
  • Organizer: ESHRE 2007 (23rd Annual Meeting of The Europian Society of Human Reproduction and Embryology)
  • Place of Presentation: Lyon, France