Combinational antitumor effect of siRNA against M phase checkpoint gene and paclitaxel on growth of human endometrial cancer

• Project/Area Number: 19791163
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Obstetrics and gynecology
• Research Institution: Keio University
• Principal Investigator: YANOKURA Megumi Keio University, 医学部, 助教 (20433732)
• Project Period (FY): 2007 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥3,680,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥480,000); Fiscal Year 2008: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2007: ¥1,600,000 (Direct Cost: ¥1,600,000)
• Keywords: アテロコラーゲン / 子宮体癌 / Aurora-A / CHFR / DDS / 細胞周期チェックポイント

Research Abstract

子宮体癌においてCHFRまたはAurora-Aの過剰発現とタキサン製剤に対する感受性との関連を明らかとした。In vitroにおいて、CHFRまたはAurora-Aが過剰発現しているヒト子宮体癌細胞株は、発現していない細胞株に比し、タキサン製剤に対する感受性が低い事実を突き止めた。CHFRまたはAurora-Aの過剰発現細胞株に各遺伝子のsiRNAを導入し、遺伝子発現抑制前後の各種抗癌剤に対する感受性の変化を測定したところ、タキサン製剤に対する感受性が特異的に増強した。

Research Products

• [Journal Article] hypermethylation of cancer-related genes in carcinogenesis of endometrial cancer (2008)
  • Author(s): Banno K, Yanokura M, Susumu N, Ao ki D. Epigenetic DNA
  • Journal Title: In: Maruo T, Mardon H, Stewart C, eds. Translatrional Research in Uterine Biology, Amsterdam : ELSEVIER Pages: 191-200
  • Peer Reviewed
• [Journal Article] Epigenetic inactivation of the CHFR gene in cervical cancer contributes to sensitivity to taxanes (2007)
  • Author(s): Banno K*, Yanokura M*, Kawaguchi M, Kuwabara Y, Akiyoshi J, Kobayashi Y, Iwata T, Hirasawa A, Fujii T, Susumu N, Tsukazaki K, Aoki D
  • Journal Title: Int J of Oncol 2007 31 Pages: 713-720
  • Peer Reviewed
• [Journal Article] Use of the collagen gel droplet embedded drug sensitivity test to determine drug sensitivity against ovarian mature cystic teratoma with malignant transformation to adenocarcinoma ; A case report (2007)
  • Author(s): Yamagami W, Banno K, Kawaguchi M, Yanokura M, Kuwabara Y, Hirao N, Susumu N, Tsukazaki K, Aoki D
  • Journal Title: Chemotherapy 53 Pages: 137-141
  • Peer Reviewed
• [Journal Article] Relationship of aberrant DNA hypermethylation of CHFR with sensitivity to taxanes in endometrial cancer (2007)
  • Author(s): Yanokura M*, Banno K*, Kawaguchi M, Hirao N, Hirasawa A, Susumu N, Tsukazaki K, Aoki D
  • Journal Title: Oncology Reports 17 Pages: 41-48
  • Peer Reviewed
• [Journal Article] Epigenetic inactivation of the CHFR gene in cervical cancercontributes to sensitivity to taxanes (2007)
  • Author(s): Banno K
  • Journal Title: Int J Oncol 31 Pages: 713-720
  • Peer Reviewed
• [Journal Article] Use of the collagen gel droplet embedded drug sensitivity test todetermine drug sensitivity against ovaxian mature cystic teratoma with malignant transformation to adenocarcinoma (2007)
  • Author(s): Yamagami W
  • Journal Title: Cheinotlierapy 53 Pages: 137-141
  • Peer Reviewed
• [Presentation] Expression of a mitotic kinase, Aurora-A (2007)
  • Author(s): 矢野倉恵
  • Organizer: affects taxane sensitivity in endometrial cancer, 20th AOCOG
  • Place of Presentation: 東京
  • Year and Date: 2007-09-24
• [Presentation] Expression of a mitotic kinase, Aurora-A, affects taxane sensitivity ill endometrial cancer (2007)
  • Author(s): 矢野 倉恵
  • Organizer: 20th AOCOG
  • Place of Presentation: 東京
  • Year and Date: 2007-09-24
• [Presentation] 子宮頸癌におけるM期チェックポイント遺伝子CHFRの異常メチル化の分子指標としての可能性 (2007)
  • Author(s): 矢野倉恵
  • Organizer: 第59回日本産科婦人科学会学術集会
  • Place of Presentation: 京都
  • Year and Date: 2007-04-16