Evaluation of egg ability for fertilization by sugar chain molecular probes

• Project/Area Number: 19791179
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Obstetrics and gynecology
• Research Institution: National Research Institute for Child Health and Development
• Principal Investigator: MIYAMOTO Kiyoko National Research Institute for Child Health and Development, 生殖・細胞医療研究部, 共同研究員 (20415590)
• Project Period (FY): 2007 – 2009
• Project Status: Completed (Fiscal Year 2009)
• Budget Amount: ¥4,080,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥780,000); Fiscal Year 2009: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2008: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2007: ¥700,000 (Direct Cost: ¥700,000)
• Keywords: 生殖医学 / 糖鎖 / レクチン / 卵子 / 受精 / ケクチン / 精子

Research Abstract

Sugar-specific probes such as lectins and antibodies have been used as a molecular probe for labeling sugar chains and evaluating cell activities. In order to evaluate egg ability in fertilization, especially fusion with sperm, we studied each of CD9-and CD81-deficient eggs. Eventually, we found that CD9-and ganglioside GM3-containing vesicles are released from eggs before fertilization, and regulate sperm-fusing ability. We named the vesicles as egg exosomes. Our results suggest that GM3-containing exosomes regulate sperm-egg fusion in mammals.

Research Products

• [Journal Article] The fusing ability of sperm is bestowed by CD9-containing vesicles released from eggs in mice. (2008)
  • Author(s): Miyado K, Yoshida K, Yamagata K, Sakakibara K, Okabe M, Wang X, Miyamoto K, Akutsu H, Kondo T, Takahashi Y, Ban T, Ito C, Toshimori K, Nakamura A, Ito M, Miyado M, Mekada E, Umezawa A.
  • Journal Title: Proc Natl Acad Sci USA 105(35) Pages: 12921-6
• [Journal Article] Possible involvement of CD81 in acrosome reaction of sperm in mice. (2008)
  • Author(s): Tanigawa M, Miyamoto K, Kobayashi S, Sato M, Akutsu H, Okabe M, Mekada E, Umezawa A, Miyado K.
  • Journal Title: Mol Reprod Dev 75(1) Pages: 150-5
• [Journal Article] The fusing ability of sperm is bestowed by CD9-containing vesicles released from eggs in mice (2008)
  • Author(s): Miyado K, Yoshida K, Yamagata K, Sakakibara K, Okabe M, Wang X, Miyamoto K, Akutsu H, Kondo T, Takahashi Y, Ban T, Ito C, Toshimori K, Nakamura A, Ito M, Miyado M, Mekada E, Umezawa A.
  • Journal Title: Proc Natl Acad Sci USA 105 Pages: 12921-6
  • Peer Reviewed
• [Journal Article] Possible involvement of CD81 in acrosome reaction of sperm in mice (2008)
  • Author(s): Tanigawa M, Mivamoto K, Kobayashi S, Sato M, Akutsu H, Okabe M, Mekada E, Umezawa A, and Miyado K.
  • Journal Title: Molecular Reproduction and Development 75 Pages: 150-155
  • Peer Reviewed