Regeneration of Inner Ear Hair Cells Through Activation of BMP Signaling

• Project/Area Number: 19791207
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Otorhinolaryngology
• Research Institution: Kyoto University
• Principal Investigator: SAKAMOTO Tatsunori Kyoto University, 医学研究科, 助教 (60425626)
• Project Period (FY): 2007 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥3,690,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥390,000); Fiscal Year 2008: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2007: ¥2,000,000 (Direct Cost: ¥2,000,000)
• Keywords: 内耳再生 / 有毛細胞 / BMP-4 / 感音難聴 / 内耳障害 / ノックアウトマウス / 耳胞培養

Research Abstract

ほ乳類では内耳有毛細胞はいったん傷害されると再生することがない。BMP-4は有毛細胞の発生に関わる重要な因子である。本研究ではBMPシグナルの操作による有毛細胞再生の可能性について検討した。BMP-4、Nogginなどは発生中の内耳での発現が確認され、耳胞培養系では培養条件を確立、有毛細胞の評価型として用いることが出来ることを確認した。

Research Products

• [Journal Article] Prostaglandin E Receptor Subtype EP4 Agonist Protects Cochleae Against Noise-Induced Trauma Neuroscience (2009)
  • Author(s): Hori R, Nakagawa T, Sugimoto Y, Sakamoto T, Yamamoto N, Hamaguchi, K, Ito J.
  • Journal Title: Neuroscience
  • Peer Reviewed
• [Journal Article] Prostaglandin E Receptor Subtype EP4 Agonist Protects Cochleae Against Noise-Induced Trauma, Neuroscience (2009)
  • Author(s): Hori R, Nakagawa T, Sugimoto Y, Sakamoto T, Yamamoto N. Hamaeuchi
  • Journal Title: Neurscience
  • Peer Reviewed
• [Journal Article] Potential of embryonic stem cell-derived neurons for synapse formation with auditory hair cells. (2008)
  • Author(s): Matsumoto M, Nakagawa T, Kojima K, Sakamoto T, Fujiyama F, Ito J.
  • Journal Title: J Neurosci Res. 86 Pages: 2075-85
  • Peer Reviewed
• [Journal Article] Surgical Invasiveness of Cell ORL J Otorhinolaryngol Relat Spec. Transplantation into the Guinea Pig (2008)
  • Author(s): Ogita H, Nakagawa T, Lee KY, Inaoka T, Okano T, Kikkawa YS, Sakamoto T, Ito J.
  • Journal Title: Cochlear Modiolus 71 Pages: 31-39
  • Peer Reviewed
• [Journal Article] Potential of embryonic stem cell-derived neurons for synapse formation with auditory hair cells (2008)
  • Author(s): Matsumoto M, Nakagawa T, Kojima K, Sakamoto T, Fujiyama F, Ito J
  • Journal Title: J Neurosci Res 86 Pages: 2075-85
  • Peer Reviewed
• [Journal Article] Surgical Invasiveness of Cell Transplantation into the Guinea Pig Cochlear Modiolus (2008)
  • Author(s): Ogita H, Nakagawa T, Lee KY, Inaoka T, Okano T, Kikkawa YS
  • Journal Title: ORL J Otorhinolaryngol Relat Spec 71 Pages: 32-39
  • Peer Reviewed
• [Journal Article] Hair cell differentiation becomes tissue specific by E9. 5 in mouse inner ear (2008)
  • Author(s): Sakamoto T, Ito J, Ladher RK
  • Journal Title: Neuroreport 18(9) Pages: 841-4
  • Peer Reviewed
• [Journal Article] Ladher RKHair cell differentiation becomes tissue specific by E9.5 in mouse inner ear. (2007)
  • Author(s): Sakamoto T, Ito J
  • Journal Title: Neuroreport 18(9) Pages: 841-4
  • Peer Reviewed
• [Journal Article] JPharmacological inhibition of Notch signaling in the mature guinea pig cochlea (2007)
  • Author(s): Hori R, Nakagawa T, Sakamoto T, Matsuoka Y, Takebayashi S, Ito
  • Journal Title: Neuroreport 18(18) Pages: 1911-14
  • Peer Reviewed
• [Journal Article] Pharmacological inhibition of Notch signaling in the mature guinea pig cochlea. (2007)
  • Author(s): Hori R, Nakagawa T*, Sakamoto T, Matsuoka Y, Takebayashi S, Ito J
  • Journal Title: Neuroreport 18(18) Pages: 1911-1914
  • Peer Reviewed
• [Presentation] Treatment of Tinnitus by Lidocaine Using Inner Ear Drug Delivery (2009)
  • Author(s): Sakamoto T, Horie R, Nakagawa T, Tabata Y, Ito J.
  • Organizer: ARO 2009 MidWinter Meeting
  • Place of Presentation: Baltimore
  • Year and Date: 2009-02-14
• [Presentation] Efficacy of Stealth Nano-Particles Encapsulating Betamethasone for Attenuation of Noise Induced Hearing Loss in Mice. (2009)
  • Author(s): Horie RT, Sakamoto T, Nakagawa T, Ito J.
  • Organizer: ARO 2009 MidWinter Meeting
  • Place of Presentation: Baltimore
  • Year and Date: 2009-02-14
• [Presentation] Treatment of Tinnitus by Lidocaine Using Inner Ear Drug Delivery (2009)
  • Author(s): Sakamoto T, Horie R, Nakagawa T, Tabata Y, Ito J
  • Organizer: ARO 2009 MidWinter Meeting
  • Place of Presentation: Baltimore, U.S.A.
  • Year and Date: 2009-02-14
• [Presentation] Efficacy of Stealth Nano-Particles Encapsulating Betamethasone for Attenuation of Noise Induced Hearing (2009)
  • Author(s): Horie RT, Sakamoto T, Nakagawa T, Ito J
  • Organizer: ARO 2009 MidWinter Meeting
  • Place of Presentation: Baltimore, U.S.A.
  • Year and Date: 2009-02-14
• [Presentation] Inner Ear Regeneration by Induced Pluripotent Stem (iPS) Cells. (2008)
  • Author(s): Sakamoto T, Nishimura K, Inaoka T, Nakagawa T, Ito J.
  • Organizer: 45th Inner Ear Biology Workshop (IEB2008).
  • Place of Presentation: Ferrara
  • Year and Date: 2008-09-21
• [Presentation] Inner Ear Regeneration by Induced Pluripotent Stem (iPS) Cells (2008)
  • Author(s): Sakamoto T, Nishimura K, Inaoka T, Nakagawa T, Ito J
  • Organizer: 45th Inner Ear Biology Workshop (IEB2008)
  • Place of Presentation: Ferrara, Itary
  • Year and Date: 2008-09-21
• [Presentation] Inner Ear Therapies Against Hearing Impairment and Tinnitus Using Inner Ear Drug Delivery Systems. (2008)
  • Author(s): Sakamoto T, Nakagawa T, Horie R, Ito J, Tabata Y, Ishiara T, Higaki M.
  • Organizer: Conference on Cell Replacement in the Inner Ear
  • Place of Presentation: Bethesda
• [Presentation] Inner Ear Therapies Against Hearing Impairment and Tinnitus Using Inner Ear Drug Delivery Systems (2008)
  • Author(s): Sakamoto T, Nakagawa T, Horie R, Ito J, Tabata Y, Ishiara T, Hicaki
  • Organizer: Conference on Cell Replacement in the Inner Ear
  • Place of Presentation: Bethesda, U.S.A.
• [Presentation] Pharmacological inhibition of Notch signaling in damaged cochleae of guinea pigs. (2007)
  • Author(s): Sakamoto T, Nakagawa T, Hori R, Matsuoka Y, Takebayashi S, Ito J.
  • Organizer: Age Related Hearing Impairment, International Congress 2007
  • Place of Presentation: アントワープ
  • Year and Date: 2007-06-17
• [Presentation] 耳胞のパターニングに対する後脳の役割 (2007)
  • Author(s): 坂本達則, ラージ・ラダ, 伊藤壽一.
  • Organizer: 第108回日本耳鼻咽喉科学会総会・学術講演会
  • Place of Presentation: 金沢
  • Year and Date: 2007-05-17
• [Presentation] Pharmacological inhibition of Notch signaling in damaged cochleae of guinea pigs (2007)
  • Author(s): Sakamoto T, Nakagawa T, Hori R, Matsuoka Y, Takebayashi S, Ito J
  • Organizer: Age Related Hearing Impairment, International Congress 2007
  • Place of Presentation: Antwerp, Belgium
  • Year and Date: 2007-05-17
• [Presentation] Hair cell differentiation becomes tissue specific by E9.5 in mouse inner ear (2007)
  • Author(s): Tatsunori Sakamoto, Juichi Ito, Raj Ladher.
  • Organizer: 44th Inner Ear Biology Workshop
  • Place of Presentation: LONDON
• [Presentation] 耳胞のパターニングに対する後脳の役割 (2007)
  • Author(s): 坂本達則, 中川隆之, 伊藤壽一
  • Organizer: 第17回日本耳科学会総会・学術講演会
  • Place of Presentation: 福岡
• [Presentation] Hair cell differentiation becomes tissue specific by E9. 5 in mouse inner ear (2007)
  • Author(s): Tatsunori Sakamoto, Juichi Ito, Raj Ladher
  • Organizer: 44th Inner Ear Biology Workshop
  • Place of Presentation: London, U. K.
• [Presentation] 耳胞のパターニングに対する後脳の役割 (2007)
  • Author(s): 坂本達則, 中川隆之, 伊藤壽一
  • Organizer: 第17回 日本耳科学会総会・学術講演会
  • Place of Presentation: 福岡
• [Book] 再生医療へ進む最先端の幹細胞研究 第2章8 内耳障害に対する細胞, 「移植治療」 (2008)
  • Author(s): 坂本達則
  • Publisher: 羊土社
• [Book] 再生医療へ進む最先端の幹細胞研究 第2章8 内耳障害に対する細胞移植治療 (2008)
  • Author(s): 坂本達則
  • Publisher: 羊土社