Ion transport system in the endolymphatic sac

• Project/Area Number: 19791208
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Otorhinolaryngology
• Research Institution: Kagawa University
• Principal Investigator: MIYASHITA Takenori Kagawa University, 医学部, 助教 (60363214)
• Project Period (FY): 2007 – 2009
• Project Status: Completed (Fiscal Year 2009)
• Budget Amount: ¥3,560,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥360,000); Fiscal Year 2009: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000); Fiscal Year 2008: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2007: ¥2,000,000 (Direct Cost: ¥2,000,000)
• Keywords: 内耳 / 内リンパ嚢 / イオン輸送 / メニエール病 / イオンチャネル / Na+ / K+-ATPase / イオンイメージング / パッチクランプ / Na^+, K^+-ATPase / パッチグランプ / Na^+, K^+ -ATPase

Research Abstract

The endolymphatic sac is believed to absorb the endolymph. This study focused on the ion transport control system in the endolymphatic sac. FXYD6 is a control factor of Na pump, and Na pump in the endolymphatic sac may produce Na+ driving force. We confirmed FXYD6 mRNA and protein expression in the endolymphatic sac by RT-PCR and immunohistochemistry. This results suggested that FXYD6 may control the ion transport in the endolymphatic sac.

Research Products

• [Journal Article] xpression and localization of 11 β- hydroxysteroid dehydrogenase (11βHSD) in the rat endolymphatic sac (2010)
  • Author(s): Kosuke Akiyama, Takenori Miyashita, Ai Matsubara, Ryuhei Inamoto, Terushige Mori, Akira Nishiyama, Nozomu Mori
  • Journal Title: Acta Oto-Laryngologica 130(2) Pages: 228-232
  • Peer Reviewed
• [Journal Article] Expression and localization of 11β-hydroxysteroid dehydrogenase (11β HSD) in the rat (2010)
  • Author(s): Akiyama Kosuke
  • Journal Title: Acta Oto-Laryngologica 130(2) Pages: 228-232
  • Peer Reviewed
• [Journal Article] Endolymphatic sac is involved in the regulation of hydrostatic pressure of cochlear endolymph (2009)
  • Author(s): Ryuhei Inamoto, Takenori Miyashita, Kosuke Akiyama, Terushige Mori, Nozomu Mori
  • Journal Title: Am J Physiol Regul Integr Comp Physiol 297
  • Peer Reviewed
• [Journal Article] The expression of P2Y 1, 2, 4, and 6 receptors in rat endolymphatic sac epithelia (2009)
  • Author(s): Mori Terushige, Miyashita Takenori, Akiyama Kosuke, Inamoto Ryuhei, Mori Nozomu
  • Journal Title: Neuroreport 20(4) Pages: 419-423
  • Peer Reviewed
• [Journal Article] The expression of P2Y 1,2,4, and 6 receptors in rat endolymphatic sac epithelia (2009)
  • Author(s): Mori Terushige
  • Journal Title: Neuroreport 20(4) Pages: 419-423
  • Peer Reviewed
• [Journal Article] Endolymphatic sac is involved in the regulation of hydrostatic pressure of cochlear endolymph (2009)
  • Author(s): Inamoto Ryuhei
  • Journal Title: Am J Physiol Regul Integr Comp 297
  • Peer Reviewed
• [Journal Article] Expression of thiazide-sensitive Na+-Cl- cotransporter in the rat endolymphatic sac (2008)
  • Author(s): Kosuke Akiyama, Takenori Miyashita, Terushige Mori, Ryuhei Inamoto, Nozomu Mori
  • Journal Title: BBRC 371 Pages: 649-653
  • Peer Reviewed
• [Journal Article] A new approach for selective rat endolymphatic sac epithelium collection to obtain pure specific RNA (2008)
  • Author(s): Kosuke Akiyama, Takenori Miyashita, Ai Matsubara, Terushige Mori, Ryuhei Inamoto, Akira Nishiyama, Nozomu Mori
  • Journal Title: BBRC 376 Pages: 611-614
  • Peer Reviewed
• [Journal Article] Expression of thiazide-sensitive Na+-Cl-cotransporter in the rat endolymphatic sac (2008)
  • Author(s): Akiyama, K., Miyashita, T., et.al.
  • Journal Title: Biochem Biophys Res Commun 371 Pages: 649-53
  • Peer Reviewed
• [Journal Article] A new approach for selective rat endolymphatic sac epit helium collection to obtain pure specific RNA (2008)
  • Author(s): Akiyama, K., Miyashita, T., et.al.
  • Journal Title: Biochem Biophys Res Commun 376 Pages: 611-4
  • Peer Reviewed
• [Journal Article] The expression of P2Y1, 2, 4, and 6 receptors in rat endolvmphatic sac epithelia (2008)
  • Author(s): Mori, T., Miyashita, T., et.al.
  • Journal Title: Neuroreport 20 Pages: 419-23
  • Peer Reviewed
• [Journal Article] Large Na+ influx and high Na+, K+ -ATPase activity in mitochondoria-rich epithelial cells of the inner ear endolymphatic sac (2007)
  • Author(s): Takenori Miyashita, Hitoshi Tatsumi, Kimihide Hayakawa, Nozomu Mori, Masahiro Sokabe
  • Journal Title: Pflugers Arch Eur J Physiol 453 Pages: 905-913
  • Peer Reviewed
• [Journal Article] Expression of the Na+-K+-2Cl- cotransporter in the rat endolymphatic sac (2007)
  • Author(s): Kosuke Akiyama, Takenori Miyashita, Terushige Mori, Nozomu Mori
  • Journal Title: BBRC 364 Pages: 913-917
  • Peer Reviewed
• [Journal Article] Large Na^+influx and high Na^+,K^+,-ATPase activity in mitochondria・rich epithelial cells of the inner ear endolymphatic sac (2007)
  • Author(s): Miyashita Takenori
  • Journal Title: Pflugers Arch Eur J Physiol 453 Pages: 905-913
  • Peer Reviewed
• [Journal Article] Expression of the Na^+-K^+-2Cr^- cotransporter in the rat endolymphaticsac (2007)
  • Author(s): Kosuke Akiyama
  • Journal Title: BBRC 364 Pages: 913-917
  • Peer Reviewed
• [Presentation] Determining the volume of the elliptical cone simulating the vestibular aqueduct in meniere's disease using multiplanar reconstruction images:A novel index for the vestibular aqueduct evaluation (2010)
  • Author(s): Takenori Miyashita, Yoshihiro Toyama, Ryuhei Inamoto, Nozomu Mori
  • Organizer: Association for Research in Otolaryngology
  • Place of Presentation: Disneyland Hotel(Anaheim, CA, USA)
  • Year and Date: 2010-02-08
• [Presentation] RNA expression of claudins in the rat endolymphatic sac (2010)
  • Author(s): Ai Matsubara, Takenori Miyashita, Terushige Mori, Ryuhei Inamoto, Nozomu Mori
  • Organizer: Association for Research in Otolaryngology
  • Place of Presentation: Disneyland Hotel(Anaheim, CA, USA)
  • Year and Date: 2010-02-08
• [Presentation] Determining the volume of the elliptical cone simulating the vestibular aqueduct in meniere's disease using multiplanar reconstruction images : A novel index for the vestibular aqueduct evaluation (2010)
  • Author(s): Takenori Miyashita
  • Organizer: Association for Research on Otolaryngology
  • Place of Presentation: Disneyland Hotel(Anaheim, CA,USA)
  • Year and Date: 2010-02-08
• [Presentation] RNA expression of claudins in the rat endolymphatic sac (2010)
  • Author(s): Ai Matsubara
  • Organizer: Association for Research on Otolaryngology
  • Place of Presentation: Disneyland Hotel(Anaheim, CA,USA)
  • Year and Date: 2010-02-08
• [Presentation] メニエール病の減塩治療における血中浸透圧変化 (2009)
  • Author(s): 宮下武憲, 稲本隆平, 森望
  • Organizer: 血中ホルモン変化と臨床所見第68回日本めまい平衡医学会
  • Place of Presentation: ホテルクレメント徳島(徳島市)
  • Year and Date: 2009-11-26
• [Presentation] カテコールアミンによる前庭内リンパ圧の変化 (2009)
  • Author(s): 稲本隆平, 宮下武憲, 森望
  • Organizer: 第68回日本めまい平衡医学会
  • Place of Presentation: ホテルクレメント徳島(徳島市)
  • Year and Date: 2009-11-26
• [Presentation] メニエール病の減塩治療における血中浸透圧変化、血中ホルモン変化と臨床所見 (2009)
  • Author(s): 宮下武憲
  • Organizer: 第68回日本めまい平衡医学会
  • Place of Presentation: ホテルクレメント徳島(徳島市)
  • Year and Date: 2009-11-26
• [Presentation] 内リンパ嚢上皮細胞におけるFXYD6の発現 (2009)
  • Author(s): 宮下武憲, 秋山貢佐, 稲本隆平, 森照茂, 森望
  • Organizer: 第19回日本耳科学会
  • Place of Presentation: 京王プラザホテル(東京都)
  • Year and Date: 2009-10-10
• [Presentation] レーザーキャプチャーマイクロダイセクションを用いた選択的内リンパ嚢由来RNA抽出方法 (2009)
  • Author(s): 秋山貢佐, 宮下武憲, 稲本隆平, 森照茂, 森望
  • Organizer: 第19回日本耳科学会
  • Place of Presentation: 京王プラザホテル(東京都)
  • Year and Date: 2009-10-10
• [Presentation] ラット内リンパ嚢における11 β-HSDの発現 (2009)
  • Author(s): 秋山貢佐, 宮下武憲, 松原あい, 稲本隆平, 森照茂, 森望
  • Organizer: 第110回日本耳鼻咽喉科学会
  • Place of Presentation: ザプリンスパークタワー東京(東京都)
  • Year and Date: 2009-05-15
• [Presentation] 内リンパ嚢の内リンパ圧調節作用に関する研究 (2009)
  • Author(s): 稲本隆平, 宮下武憲, 森望
  • Organizer: 第110回日本耳鼻咽喉科学会
  • Place of Presentation: ザプリンスパークタワー東京(東京都)
  • Year and Date: 2009-05-15
• [Presentation] ラット内リンパ嚢における11β-HSDの発現 (2009)
  • Author(s): 秋山貢佐
  • Organizer: 第110回日本耳鼻咽喉科学会
  • Place of Presentation: ザプリンスパークタワー東京(東京都)
  • Year and Date: 2009-05-15
• [Presentation] メニエール病の減塩治療-血中ホルモン変化と臨床所見- (2009)
  • Author(s): 宮下武憲, 稲本隆平, 森望
  • Organizer: 第110回日本耳鼻咽喉科学会
  • Place of Presentation: ザプリンスパークタワー東京(東京都)
  • Year and Date: 2009-05-14
• [Presentation] Na+ influx in mitochondria-rich epithelial cells of the inner ear endolymphatic sac (2009)
  • Author(s): Takenori Miyashita, Hitoshi Tatsumi, Kimihide Hayakawa, Masahiro Sokabe, Nozomu Mori
  • Organizer: 6th Extraordinary International Symposium on Recent Advances
  • Place of Presentation: Lotte Hotel Seoul (Seoul Korea)
  • Year and Date: 2009-05-09
• [Presentation] Na+ influx in mitochondria-rich epithelial cells of the inner ear endolymphatic sac (2009)
  • Author(s): Takenori Miyashita
  • Organizer: 6^<th> Extraordinary international symposium on recent advances
  • Place of Presentation: Lotte Hotel Seoul (Seoul Korea)
  • Year and Date: 2009-05-09
• [Presentation] Presence of the FXYD6 in the endolymphatic sac epithelia (2009)
  • Author(s): Takenori Miyashita, Kosuke Akiyama, Ryuhei Inamoto, Terushige Mori, Nozomu Mori
  • Organizer: 32nd Association for Research in Otolaryngology MidWinter Meeting
  • Place of Presentation: Baltimore Marriott Waterfront (Baltimore, Maryland, USA)
  • Year and Date: 2009-02-17
• [Presentation] 11 β-hydroxysteroid dehydrogenase (11βHSD) expression in the rat endolymphatic sac (2009)
  • Author(s): Kosuke Akiyama, Takenori Miyashita, Ai Matsubara, Ryuhei Inamoto, Terushige Mori, Akira Nishiyama, Nozomu Mori
  • Organizer: 32nd Association for Research in Otolaryngology MidWinter Meeting
  • Place of Presentation: Baltimore Marriott Waterfront (Baltimore, Maryland, USA)
  • Year and Date: 2009-02-17
• [Presentation] Endolymphatic sac is involved in the regulation of endolymphatic pressure (2009)
  • Author(s): Ryuhei Inamoto, Takenori Miyashita, Nozomu Mori
  • Organizer: 32nd Association for Research in Otolaryngology MidWinter Meeting
  • Place of Presentation: Baltimore Marriott Waterfront (Baltimore, Maryland, USA)
  • Year and Date: 2009-02-17
• [Presentation] 11β-hydroxysteroid dehydrogenase (11β HSD) expression in the rat endolymphatic sac (2009)
  • Author(s): Akiyama K, Miyashita T., et.al.
  • Organizer: 32nd Association for Research in Otolaryngology MidWinter Meeting
  • Place of Presentation: Baltimore, Maryland, USA
  • Year and Date: 2009-02-17
• [Presentation] Endolymphatic sac is involved in the regulation of endolymphatic pressure (2009)
  • Author(s): Inamoto R, Miyashita T., et.al.
  • Organizer: 32nd Association for Research in Otolaryngology MidWinter Meeting
  • Place of Presentation: Baltimore, Maryland, USA
  • Year and Date: 2009-02-17
• [Presentation] 内リンパ嚢の内リンパ圧調節作用に関する研究 (2008)
  • Author(s): 稲本隆平, 宮下武憲, 森望
  • Organizer: 日本耳鼻咽喉科学会第34回四国四県地方部会連合学会
  • Place of Presentation: 高知市文化プラザかるぽーと(高知市)
  • Year and Date: 2008-12-07
• [Presentation] ラット内リンパ嚢における11 β-HSDの発現とアルドステロンの関連 (2008)
  • Author(s): 秋山貢佐, 宮下武憲, 松原あい, 稲本隆平, 森照茂, 森望, 西山成
  • Organizer: 日本耳鼻咽喉科学会第34回四国四県地方部会連合学会
  • Place of Presentation: 高知市文化プラザかるぽーと(高知市)
  • Year and Date: 2008-12-07
• [Presentation] ラット内リンパ嚢における11β-HSDの発現とアルドステロンの関連 (2008)
  • Author(s): 秋山貢佐、宮下武憲, 他
  • Organizer: 日本耳鼻咽喉科学会第34回四国四県地方部会連合学会
  • Place of Presentation: 高知市
  • Year and Date: 2008-12-07
• [Presentation] 内リンパ嚢の内リンパ圧調節作用に関する研究 (2008)
  • Author(s): 稲本隆平、宮下武憲、森望
  • Organizer: 日本耳鼻咽喉科学会第34回四国四県地方部会連合学会
  • Place of Presentation: 高知市
  • Year and Date: 2008-12-07
• [Presentation] メニエール病における減塩治療時の血中ホルモン動態 (2008)
  • Author(s): 宮下武憲, 稲本隆平, 森望
  • Organizer: 第18回日本耳科学会
  • Place of Presentation: 神戸国際会議場(神戸市)
  • Year and Date: 2008-10-17
• [Presentation] 内リンパ嚢におけるNa-Cl共輸送体の発現 (2008)
  • Author(s): 秋山貢佐, 宮下武憲, 森照茂, 稲本隆平, 森望
  • Organizer: 第18回日本耳科学会
  • Place of Presentation: 神戸国際会議場(神戸市)
  • Year and Date: 2008-10-17
• [Presentation] カテコールアミンによる蝸牛内リンパ圧の変化 (2008)
  • Author(s): 稲本隆平, 宮下武憲, 森望
  • Organizer: 第18回日本耳科学会
  • Place of Presentation: 神戸国際会議場(神戸市)
  • Year and Date: 2008-10-17
• [Presentation] メニエール病における減塩治療時の血中ホルモン動態 (2008)
  • Author(s): 宮下武憲, 他
  • Organizer: 第18回日本耳科学会
  • Place of Presentation: 神戸市
  • Year and Date: 2008-10-17
• [Presentation] カテコールアミンによる蝸牛内リンパ圧の変化 (2008)
  • Author(s): 稲本隆平、宮下武憲、森望
  • Organizer: 第18回日本耳科学会
  • Place of Presentation: 神戸市
  • Year and Date: 2008-10-17
• [Presentation] 内リンパ嚢におけるNa-Cl共輸送体の発現 (2008)
  • Author(s): 秋山貢佐、宮下武憲, 他
  • Organizer: 第18回日本耳科学会
  • Place of Presentation: 神戸市
  • Year and Date: 2008-10-17
• [Presentation] Presence of the FXYD6 in the endolymphatic sac epithelia (2008)
  • Author(s): Miyashita Takenori, Akiyama Kosuke, Inamoto Ryuhei, Mori Terushige, Mori Nozomu
  • Organizer: 45th Inner Ear Biology
  • Place of Presentation: Sala S. Francesco (Ferrara Italy)
  • Year and Date: 2008-09-23
• [Presentation] Expression of the Na-K-2Cl cotransportor(NKCC-2) in the rat endolymphatic sac (2008)
  • Author(s): Akiyama Kosuke, Miyashita Takenori, Mori Terushige, Inamoto Ryuhei, Mori Nozomu
  • Organizer: 45th Inner Ear Biology
  • Place of Presentation: Sala S. Francesco (Ferrara Italy)
  • Year and Date: 2008-09-23
• [Presentation] The effect of isoproterenol on the hydrostatic pressure of cochlear endolymph (2008)
  • Author(s): Inamoto Ryuhei, Miyashita Takenori, Akiyama Kosuke, Mori Terushige, Mori Nozomu
  • Organizer: 45th Inner Ear Biology
  • Place of Presentation: Sala S. Francesco (Ferrara Italy)
  • Year and Date: 2008-09-23
• [Presentation] Expression of P2Y receptors in the rat endolymphatic sac (2008)
  • Author(s): Mori Terushige, Miyashita Takenori, Akiyama Kosuke, Inamoto Ryuhei, Mori Nozomu
  • Organizer: 45th Inner Ear Biology
  • Place of Presentation: Sala S. Francesco (Ferrara Italy)
  • Year and Date: 2008-09-23
• [Presentation] Presence of the FXYD6 in the endolymphatic.sac epithelia (2008)
  • Author(s): Miyashita T., et.al.
  • Organizer: 45^<th> Inner Ear Biology
  • Place of Presentation: Ferrara Italy
  • Year and Date: 2008-09-23
• [Presentation] The effect of isoproterenol on the hydrostatic pressure of cochlear endolymph (2008)
  • Author(s): Inamoto R, Miyashita T., et.al.
  • Organizer: 45^<th> Inner Ear Biology
  • Place of Presentation: Ferrara Italy
  • Year and Date: 2008-09-23
• [Presentation] Expression of P2Y receptors in the rat endolymphatic sac (2008)
  • Author(s): Mori Terushige, Miyashita T., et.al.
  • Organizer: 45th Inner Ear Biology
  • Place of Presentation: Ferrara Italy
  • Year and Date: 2008-09-23
• [Presentation] Expression of the Na-K-2Cl cotransportor(NKCC-2) in the rat endolymphatic sac (2008)
  • Author(s): Akiyama K, Miyashita T., et.al.
  • Organizer: 45^<th> Inner Ear Biology
  • Place of Presentation: Ferrara Italy
  • Year and Date: 2008-09-23
• [Presentation] 内リンパ嚢の内リンパ圧調節における役割に関する研究 (2008)
  • Author(s): 稲本隆平, 宮下武憲, 森望
  • Organizer: 第26回頭頸部自律神経研究会
  • Place of Presentation: ホテルグランヴィア大阪(大阪市)
  • Year and Date: 2008-08-23
• [Presentation] 内リンパ嚢の内リンパ圧調節における役割に関する研究 (2008)
  • Author(s): 稲本隆平、宮下武憲、森望
  • Organizer: 第26回頭頸部自律神経研究会
  • Place of Presentation: 大阪市
  • Year and Date: 2008-08-23
• [Presentation] カテコールアミンによる蝸牛内リンパ圧の変化 (2008)
  • Author(s): 稲本隆平, 宮下武憲, 森望
  • Organizer: 日本耳鼻咽喉科学会第34回中国四国地方部会連合学会
  • Place of Presentation: 米子市文化ホール(米子市)
  • Year and Date: 2008-05-31
• [Presentation] カテコールアミンによる蝸牛内リンパ圧の変化 (2008)
  • Author(s): 稲本隆平、宮下武憲、森望
  • Organizer: 日本耳鼻咽喉科学会第34回中国四国地方部会連合学会
  • Place of Presentation: 米子市
  • Year and Date: 2008-05-31
• [Presentation] Presence of the FXYD6 in the endo1ymphatio sac epithelia (2008)
  • Author(s): Miyashita T., et.al.
  • Organizer: 32nd Association for Research in Otolaryngology MidWinter Meeting
  • Place of Presentation: Baltimore, Maryland, USA
  • Year and Date: 2008-02-17