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Identification and differentiation induction of M cell in human nasal epithelial cells: a unique and new target for DDS

Research Project

Project/Area Number 19791218
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeSingle-year Grants
Research Field Otorhinolaryngology
Research InstitutionSapporo Medical University

Principal Investigator

GO Mitsuru  Sapporo Medical University, 医学部, 助教 (80448604)

Project Period (FY) 2007 – 2008
Project Status Completed (Fiscal Year 2008)
Budget Amount *help
¥3,690,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2008: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2007: ¥2,000,000 (Direct Cost: ¥2,000,000)
Keywords耳鼻咽喉学 / アレルギー / 病理学 / タイト結合 / 鼻粘膜
Research Abstract

ヒト咽頭扁桃(adenoid)上皮は,発達したタイト結合による上皮バリア機能を有し,外来病原体に対する生体防御の役割を担っていることが考えられる.そこで,我々はcytokeratin 20 (Ck20)をマーカーとして,ヒト咽頭扁桃(adenoid)におけるM細胞の同定をし,in vivoおよびin vitroでのタイト結合蛋白の発現を調べた.

Report

(3 results)
  • 2008 Annual Research Report   Final Research Report ( PDF )
  • 2007 Annual Research Report
  • Research Products

    (23 results)

All 2009 2008 2007 Other

All Journal Article (18 results) (of which Peer Reviewed: 15 results) Presentation (5 results)

  • [Journal Article] Drug Delivery System (DDS)としてのエアロゾルの将来性鼻粘膜を介した新たな治療戦略の開発と応用粘膜のバリアと抗原サンプリング鼻粘膜を介した新たな治療戦略にむけて.2008

    • Author(s)
      郷充,小島隆,亀倉隆太,小笠原徳子,小泉純一,黒瀬誠,高野賢一,澤田典均,氷見徹夫
    • Journal Title

      耳鼻咽喉科展望 51(補1)

      Pages: 32-38

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] PKC enhances tight junction barrier function of human nasal epithelial cells in primary culture by transcriptional regulation.2008

    • Author(s)
      Koizumi J, Kojima T, Ogasawara N, Kamekura R, Kurose M, Go M, Harimaya A, Murata M, Osanai M, Chiba H, Himi T, Sawada N.
    • Journal Title

      74(2)

      Pages: 432-442

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Expression of tight junction proteins in epithelium including Ck20-positive M-like cells of human adenoids in vivo and in vitro.2008

    • Author(s)
      Takano K, Kojima T, Ogasawara N, Go M, Kikuchi S, Ninomiya T, Kurose M, Koizumi J, Kamekura R, Murata M, Tanaka S, Chiba H, Himi T, Sawada N.
    • Journal Title

      J Mol Histol. 39(3)

      Pages: 265-273

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Expression of thymic stromal lymphopoietin (TSLP) in allergic rhinitis : Induction of tight junction proteins in nasal epithelial cells and dendritic cells by epithelial-derived TSLP.2008

    • Author(s)
      Kamekura R, Kojima T, Koizumi J, Ogasawara N, Kurose M, Takano K, Go M, Himi T, Sawada N.
    • Journal Title

      Inflammation and Regeneration. 28(3)

      Pages: 160-165

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] 粘膜のバリアと抗原サンプリング-鼻粘膜を介した新たな治療戦略にむけて-2008

    • Author(s)
      郷充
    • Journal Title

      耳鼻咽喉科展望 51 : 補1

      Pages: 32-38

    • NAID

      130004911620

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] PKC enhances tight junction barrier function of human nasal epithelial cells in primary culture by transcriptional regulation2008

    • Author(s)
      Koizumi J
    • Journal Title

      Mol Pharmacol 74(1)

      Pages: 432-442

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Expression of tight junction proteins in epithelium including Ck20-pos itive M-like cells of human adenoids in vivo and in vitro2008

    • Author(s)
      Takano K
    • Journal Title

      J Mol Histol 39(3)

      Pages: 265-273

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Expression of thymic stromal lymphopoietin (TSLP) in allergic rhinitis : Induction of tight junction proteins in nasal epithelial cells and dendritic cells by epithelial-derived TSLP2008

    • Author(s)
      Kamekura R
    • Journal Title

      Inflammation and Regeneration 28(3)

      Pages: 160-165

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] アレルギー性鼻炎における鼻粘膜上皮バリアと抗原認識・提示機構.2007

    • Author(s)
      氷見徹夫,郷充,高野賢一,黒瀬誠,小泉純一,亀倉隆太,小島隆,澤田典均
    • Journal Title

      臨床免疫・アレルギー科 47(4)

      Pages: 427-433

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] アレルギー性鼻炎における鼻粘膜上皮バリアと免疫調節機構.2007

    • Author(s)
      氷見徹夫,郷充,高野賢一,黒瀬誠,小泉純一,亀倉隆太,小笠原徳子,小島隆,澤田典均
    • Journal Title

      アレルギーの臨床 27(13)

      Pages: 1038-1043

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] ヒト鼻粘膜上皮における自然免疫に関与するタイト結合の調節機構.2007

    • Author(s)
      小泉純一,小島隆,播摩谷敦,今野信宏,黒瀬誠,高野賢一,亀倉隆太,郷充,澤田典均,氷見徹夫
    • Journal Title

      耳鼻咽喉科免疫アレルギー 25(2)

      Pages: 65-66

    • Related Report
      2008 Final Research Report
  • [Journal Article] アレルギー性鼻炎におけるThymic stromal lymphopoietin(TSLP)の発現と上皮バリア機能への影響2007

    • Author(s)
      亀倉隆太,小島隆,黒瀬誠,小泉純一,高野賢一,今野信宏,播摩谷敦,郷充,澤田典均,氷見徹夫
    • Journal Title

      耳鼻咽喉科免疫アレルギー 25(2)

      Pages: 63-64

    • Related Report
      2008 Final Research Report
  • [Journal Article] アレルギー性鼻炎におけるThymic stromal lymphopoietin(TSLP)の役割2007

    • Author(s)
      亀倉 隆太
    • Journal Title

      第19回気道病態シンポジウム (in press)

    • Related Report
      2007 Annual Research Report
  • [Journal Article] 【鼻アレルギーの病態解明と治療の進歩】アレルギー性鼻炎における鼻粘膜上皮バリアと抗原認識・提示機構2007

    • Author(s)
      氷見 徹夫
    • Journal Title

      臨床免疫・アレルギー科 (in press)

      Pages: 427-433

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Induction of claudins in passaged hTERT-transfected human nasal epithelial cells with an extended life man2007

    • Author(s)
      Kurose M.
    • Journal Title

      Cell Tissue Res. 330(1)

      Pages: 63-74

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Expression of thymic stromal lymphopoietin(TSLP)in allergic rhiniti s:Induction of tight junction proteins in nasal epithelial cells and d endritic cells by epithelial-derived TSLP

    • Author(s)
      Kamekura R.
    • Journal Title

      Inflammation and Regeneration (in press)

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] PKC enhances tight junction barrier function of human nasal epithelia 1.cells in primary culture by transcriptional reaulation

    • Author(s)
      Koizumi J.
    • Journal Title

      Mol Pharmacol (in press)

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Expression of tight junction proteins in epithelium including Ck20-pos itive M-like cells of human adenoids in vivo and in vitro

    • Author(s)
      Takano K.
    • Journal Title

      J Mol Histol (in press)

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Presentation] Drug Delivery System (DDS)としてのエアロゾルの将来性-鼻粘膜を介した新たな治療戦略の開発と応用-粘膜のバリアと抗原サンプリング.2009

    • Author(s)
      郷充,小島隆,黒瀬誠,亀倉隆太,澤田典均,氷見徹夫
    • Organizer
      第31回日本医用エアロゾル研究会
    • Place of Presentation
      旭川市
    • Related Report
      2008 Final Research Report
  • [Presentation] 核内受容体PPARγを介したアレルギー性鼻炎の予防・治療のための基礎研究2008

    • Author(s)
      小笠原徳子
    • Organizer
      第29回日本炎症・再生医学会
    • Place of Presentation
      東京都
    • Year and Date
      2008-07-09
    • Related Report
      2008 Annual Research Report
  • [Presentation] ヒトアデノイドのCk20陽性M細胞におけるタイト結合タンパクの発現2008

    • Author(s)
      高野賢一
    • Organizer
      第109回日本耳鼻咽喉科学会総会・学術講演会
    • Place of Presentation
      大阪府
    • Year and Date
      2008-05-16
    • Related Report
      2008 Annual Research Report
  • [Presentation] 核内受容体PPAR-gammaを介したヒト鼻粘膜上皮細胞および樹状細胞のタイト結合の発現調節機構2008

    • Author(s)
      小笠原徳子,小島隆,郷充,亀倉隆太,高野賢一,大国毅,澤田典均,氷見徹夫.
    • Organizer
      第58回日本アレルギー学会秋季学術大会
    • Place of Presentation
      東京都
    • Related Report
      2008 Final Research Report
  • [Presentation] Drug Dehvery System(DDS)としてのエアロゾルの将来性2007

    • Author(s)
      郷 充
    • Organizer
      第31回日本医用エアロゾル研究会
    • Place of Presentation
      旭川市
    • Year and Date
      2007-09-22
    • Related Report
      2007 Annual Research Report

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Published: 2007-04-01   Modified: 2016-04-21  

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