Hearing disorder in osteoporotic and osteopetrotic mice

• Project/Area Number: 19791234
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Otorhinolaryngology
• Research Institution: Keio University
• Principal Investigator: KANZAKI Sho Keio University, 医学部, 助教 (50286556)
• Research Collaborator: MATSUO Koichi 慶應義塾大学, 医学部, 准教授 (40229422)
• Project Period (FY): 2007 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥3,780,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥480,000); Fiscal Year 2008: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2007: ¥1,700,000 (Direct Cost: ¥1,700,000)
• Keywords: 聴覚 / 耳小骨 / 骨代謝 / 破骨細胞 / Opg / 骨粗鬆症 / 中耳疾患

Research Abstract

破骨細胞が増加しているOpgKOマウス(骨粗鬆症マウス)は難聴を呈するが、骨粗鬆症薬を投与し形態、機能ともに予防させた。また破骨細胞が減少している大理石病モデルマウスでも難聴があることを見いだした。この結果、骨粗鬆症を有する難聴者に対して骨粗鬆症薬を投与する臨床研究を開始している。

Research Products

• [Journal Article] Cost-effectiveness of Different Treatment Modalities for Seasonal Allergic Rhinitis in Japan-Comparison of Laser Treatment with Subcutaneous Immunotherapy and Oral (2009)
  • Author(s): Taniguchi Y , Ohshiro T, Fujii S, Ohshiro T, Sasaki K, Suzuki T, Kanzaki S, Ogawa K
  • Journal Title: Medication-Laser therapy (in press)
  • Peer Reviewed
• [Journal Article] Bisphosphonate Therapy Ameliorates Hearing Loss in Mice Lacking Osteoprotegerin (2009)
  • Author(s): Kanzaki S, Takada Y, Ogawa K, Matsuo K
  • Journal Title: J Bone Miner Res 24(1) Pages: 43-9
  • Peer Reviewed
• [Journal Article] Pathogenesis of hearing loss from the point of view of the long-term prognosis of steroid-dependent sensorineural hearing loss (2009)
  • Author(s): Kanzaki J, Kanzaki S, Ogawa K
  • Journal Title: Audiol Neurotol 14(1) Pages: 26-34
  • Peer Reviewed
• [Journal Article] Bisphosphonate Therapy Ameliorates Hearing Loss in Mice Lacking Osteoprotegerin (2009)
  • Author(s): Kanzaki S, Takada Y, Ogawa K, Matsuo K
  • Journal Title: J Bone Miner Res 24 Pages: 43-49
  • Peer Reviewed
• [Journal Article] Pathogenesis of hearing loss from the point of view of the long-term prognosis of steroid-dependent sensorineural hearing loss (2009)
  • Author(s): Kanzaki J, Kanzaki S, Ogawa K
  • Journal Title: Audiol Neurotol 14 Pages: 26-34
  • Peer Reviewed
• [Journal Article] Detection system for transplanted bone marrow stem cell in inner ear SPIO (2009)
  • Author(s): Yamashita D, Watada Y, Kanzaki Toyoda M, Ogawa K, et.al.
  • Journal Title: Neurosci Res (supplement) Pages: 25-25
• [Journal Article] Bone marrow stem cells therapy for cochlear hair cell and audit function (2009)
  • Author(s): Kanzaki S, Yamashita D, Toyo M, Kobayashi M, Ogawa K, et.al.
  • Journal Title: Neurosci Res 19
• [Journal Article] Inferior meatal antrostomy impairs dynamic stability of the orbital wall (2009)
  • Author(s): Nagasao T, Miyamoto J, Tamak Kanzaki S, Ogawa K
  • Journal Title: Auris Nasus Larynx (In press)
  • Peer Reviewed
• [Journal Article] Detection system for transplanted bone marrow stem cell in inner ear by SPIO (2008)
  • Author(s): Yamashita D, Watada Y, Kanzaki S, Toyoda M, Tanimoto N, Kobayashi M, Ogawa K, Umezawa A
  • Journal Title: Neurosci Res
  • Peer Reviewed
• [Journal Article] Bone marrow stem cells therapy for cochlear hair cell and auditory function (2008)
  • Author(s): Kanzaki S ,Yamashita D, Toyoda M, Kobayashi M, Ogawa K, Umezawa A
  • Journal Title: Neurosci Res
  • Peer Reviewed
• [Journal Article] Inferior meatal antrostomy impairs dynamic stability of the orbital walls (2008)
  • Author(s): Nagasao T, Miyamoto J, Tamaki T, Kanzaki S, Ogawa K
  • Journal Title: Auris Nasus Larynx Nov.12(Epub ahead of print)
  • Peer Reviewed
• [Journal Article] Ogawa K.GFAP aggregates in the cochlear nerve increase the noise vulnerability of sensory cells in the organ of Corti in the murine model of Alexander disease (2008)
  • Author(s): Masuda M, Tanaka K, Kanzaki S, Wakabayashi K, Oishi N, Suzuki T, Ikenaka K
  • Journal Title: Neurosci Res 62(1) Pages: 15-24
  • Peer Reviewed
• [Journal Article] Mucoepidermoid carcinoma of the head and neck : clinical analysis of 43 patients (2008)
  • Author(s): Ozawa H, Tomita T, Sakamoto K, Tagawa T, Fujii R, Kanzaki S, Ogawa K, Kameyama K, Fujii M
  • Journal Title: Jpn J Clin Oncol 38(6) Pages: 414-418
  • Peer Reviewed
• [Journal Article] Neuroprotective effects of T-817MA against noise-induced hearing loss (2008)
  • Author(s): Yamashita D, Shiotani A, Kanzaki S, Nakagawa M, Ogawa K
  • Journal Title: Neurosci Res 61(1)
  • Peer Reviewed
• [Journal Article] In vivo delivery of adeno-associated virus was successfully and noninvasively transferred into the upporting cells of the neonatal mouse cochlea (2008)
  • Author(s): Iizuka T, Kanzaki S, Mochizuki H, Inoshita A, Narui Y, Furukawa M, Ogawa K, Ikeda K
  • Journal Title: Human gene ther 19(4) Pages: 384-390
  • Peer Reviewed
• [Journal Article] Bcl-2 genes regulate noise-induced hearing loss (2008)
  • Author(s): Yamashita D, Minami SB, Kanzaki S, Ogawa K, Miller JM
  • Journal Title: J Neurosci Res 86(4) Pages: 920-928
  • Peer Reviewed
• [Journal Article] In vivo delivery of adeno-associated virus was successfully and noninvasi transferred into the supporting cells of the neonatal mouse cochlea (2008)
  • Author(s): lizuka T, Kanzaki S, Mochizuki H, Inoshita A, Narui Y, et.al.
  • Journal Title: Human Gene Ther 19 Pages: 384-390
  • Peer Reviewed
• [Journal Article] Mucoepidermoid carcinoma of the head and neck: clinical analysis of patients (2008)
  • Author(s): Ozawa H, Tomita T, Sakamoto Tagawa T, Fujii R, Kanzaki S, et.al.
  • Journal Title: J Clin Oncol 38 Pages: 414-418
  • Peer Reviewed
• [Journal Article] GFAP aggregates in the cochlear nerve increase the noise vulnerability of senE cells in the organ of Corti in the murine model of Alexander disease (2008)
  • Author(s): Masuda M, Tanaka K, Kanzaki S. Oqawa K, et.al.
  • Journal Title: Neurosci Res 62 Pages: 15-24
  • Peer Reviewed
• [Journal Article] Neuroprotective effects of T-817MA against noise-induced hearing loss (2008)
  • Author(s): Yamashita D, Shiotani A, Kanzaki S, Ogawa K
  • Journal Title: Neurosci Res 61 Pages: 38-42
  • Peer Reviewed
• [Journal Article] Bcl-2 genes regulate noise-induced hearing loss (2008)
  • Author(s): Yamashita D, Minami SB, Kan2 S, Ogawa K, Miller JM., et.al.
  • Journal Title: J Neurosci Res 86 Pages: 920-928
  • Peer Reviewed
• [Journal Article] Standardised method of selecting surgical approaches to benparapharyng space tumours, based on pre-operative images (2008)
  • Author(s): Kanzaki S, Nameki H
  • Journal Title: J Laryngol Otol 122 Pages: 628-34
  • Peer Reviewed
• [Journal Article] Neuroprotective effects of T-817MA against noise-induced hearing loss. (2008)
  • Author(s): Yamashita D, Shiotani A Kanzaki S, Nakagawa M, Ogawa K.
  • Journal Title: Neuroscience Research (in press)
  • Peer Reviewed
• [Journal Article] In vivo delivery of adeno-associated virus was successfully and noninvasively transferred into the supporting cells of the neonata l mouse cochlea In vivo delivery of adeno-associated virus was successfully and noninvasively transferred into the supporting cells of the neonatal mouse cochlea. (2008)
  • Author(s): lizuka T, Kanzaki S, Mochizuki H, lnoshita A, Narui Y, Furukawa M, Ogawa k, lkeda K,
  • Journal Title: Human gene therapy (in press)
  • Peer Reviewed
• [Journal Article] Standardised method of selecting surgical approaches to benign parapharyngeal space tumours, based on pre-operative images (2007)
  • Author(s): Kanzaki S, Nameki H
  • Journal Title: J Laryngol Otol 26 Pages: 26-26
  • Peer Reviewed
• [Journal Article] Development of solitary plasmacytoma in the internal auditory canal and inner ear after allogeneic hematopoietic stem cell transplantation for plasma cell leukemia (2007)
  • Author(s): Masuda M, Inoue Y, Tanosaki R, Kanzaki S, Ogawa K
  • Journal Title: Japanese Journal of Clinical Oncology 37(9) Pages: 701-703
  • Peer Reviewed
• [Journal Article] Sendai Virus Vector-Mediated Transgene Expression in the Cochlea in vivo (2007)
  • Author(s): Kanzaki S, Shiotani A, Inoue M, Hasegawa M, Ogawa K
  • Journal Title: Audiology and Neurotology 12 Pages: 119-126
  • Peer Reviewed
• [Journal Article] Cholesterol granuloma surrounding the endolymphatic sac (2007)
  • Author(s): Kanzaki S, Araki Y, Okamoto Y, Kurita A, Ogawa K
  • Journal Title: Auris Nasus larynx 34(1) Pages: 95-100
  • Peer Reviewed
• [Journal Article] Cholesterol granuloma surrounding the endolympatic sac. (2007)
  • Author(s): Kanzaki S, Araki Y, OkamotoY, Kurita A, Ogawa K.
  • Journal Title: Auris Nasus larynx 34 Pages: 95-100
  • Peer Reviewed
• [Journal Article] Sendai Virus Vector-Mediated Transgene Expression in the Cochlea vivo. (2007)
  • Author(s): Kanzaki S, Shiotani A, Inoue M, Hasegawa M, Ogawa K.
  • Journal Title: Audiology and Neurotology 12 Pages: 119-126
  • Peer Reviewed
• [Journal Article] (研究総説)骨吸収過剰と骨吸収過剰における中耳疾患の病態について-Opgノツクアウトマウスを用いた検討から- (2007)
  • Author(s): 神崎 晶
  • Journal Title: otology Japan 17 Pages: 168-172
• [Journal Article] standardised method of selecting surgical approaches to benign parapharyngeal space tumours, based on pre-operative images. (2007)
  • Author(s): Kanzaki S, Nameki H.
  • Journal Title: J Laryngology otology 26 Pages: 1-7
  • Peer Reviewed
• [Journal Article] Development of solitary plasmacytoma in the internal auditory canal and inner ear after allogeneic hematopoietic stem cell transplantation for plasma cell leukemia. (2007)
  • Author(s): Masuda M, Inoue Y; Tanosaki
  • Journal Title: Japanese Journal of Clinical Oncology 37 Pages: 701-703
  • Peer Reviewed
• [Presentation] Noninvasive in vivo delivery of transgene via adeno-associated virus into supporting cells of the neonatal mouse cochlea (2009)
  • Author(s): Iizuka T, Kanzaki S, Ogawa K
  • Place of Presentation: ARO,Baltimore
  • Year and Date: 2009-02-16
• [Presentation] Noninvasive in vivo delivery of transgene via adeno-associated virus isupporting cells of the neonatal mouse cochlea (oral presentation) (2009)
  • Author(s): Iizuka T, Kanzaki S, Ogawa K, et.al.
  • Organizer: ARO 32^<nd> MidWinter meeting
  • Place of Presentation: Baltimore USA
• [Presentation] 突発性難聴患者における初診時血液検査と聴力予後に関する検討、神戸 (2008)
  • Author(s): 神崎晶、阪上雅史、細井祐司、村上信五、吉原俊雄、小川郁
  • Organizer: 第18回日本耳科学会
  • Place of Presentation: 神戸、日本
  • Year and Date: 2008-10-17
• [Presentation] Gene and cell delivery into nner ear (2008)
  • Author(s): Kanzaki S
  • Organizer: Keio Medical society meeting (oral)
  • Place of Presentation: Tokyo, Japan
  • Year and Date: 2008-10-14
• [Presentation] Gene and cell delivery into inner ear (oral presentation) (2008)
  • Author(s): Kanzaki S
  • Organizer: Keio Medical society meeting
  • Place of Presentation: Tokyo, Japan
  • Year and Date: 2008-10-14
• [Presentation] Bone marrow stem cells therapy for cochlear hair cell and auditory neuron Symposia"Regeneration of Sensory Cells in the Inner Ear-From Bench to Bedside-" (2008)
  • Author(s): Kanzaki S, Yamashita D, Ogawa K
  • Organizer: (oral) 31st Annual meeting Japan Neuroscience society meeting
  • Place of Presentation: Tokyo, Japan
  • Year and Date: 2008-07-11
• [Presentation] symposia "Regeneration of Sensory Cells in the Inner Ear -Fr Bench to Bedside-", (oral) (2008)
  • Author(s): Kanzaki S, Yamashita D, Ogawa K
  • Organizer: 31^<st> Annual meeting Japan Neuroscience society meeting
  • Place of Presentation: Tokyo, Japan
  • Year and Date: 2008-07-11
• [Presentation] Bisphosphonate therapy ameliorates bone resorption and hearing loss in mice lacking osteoprotegerin (2008)
  • Author(s): Kanzaki S, Ogawa K
  • Organizer: (oral), 8th Cholesteatoma and Ear Surgery meeting
  • Place of Presentation: Antalya, Turkey
  • Year and Date: 2008-06-17
• [Presentation] Bisphosphonate therapy ameliorates bone resorption and hearing ICIE mice lacking osteoprotegerin" (oral presentation) (2008)
  • Author(s): Kanzaki S, Ogawa K
  • Organizer: 8^<th> Cholesteatoma and Ear Surgery meeting
  • Place of Presentation: Antalya, Turkey
  • Year and Date: 2008-06-17
• [Presentation] (セッション老人性難聴と基礎研究)骨粗鬆症と耳小骨の変化に関する検討 (2008)
  • Author(s): 神崎晶、小川郁
  • Organizer: 東京アンチエイジングアカデミーセミナー
  • Place of Presentation: 東京、日本
  • Year and Date: 2008-06-05
• [Presentation] Does Osteoporosis Affect Morphology of Otoconia (2008)
  • Author(s): Kanzaki S, Ogawa K
  • Organizer: (oral) XXV Barany society meeting
  • Place of Presentation: Kyoto Japan
  • Year and Date: 2008-04-02
• [Presentation] Does Osteoporosis Affect Morphology of Otoconia? (oral) (2008)
  • Author(s): Kanzaki S, Ogawa K
  • Organizer: XXV Barany society meeting
  • Place of Presentation: Kyoto Japan
  • Year and Date: 2008-04-02
• [Presentation] The role of inflammation and apoptosis in sudden sensorineural hearing loss(oral) (2008)
  • Author(s): Ogawa K, Kanzaki S, Okamoto Y, Fujioka M, Matsunaga T
  • Organizer: Collegium, Oto-Rhino- Laryngologicum, Amicitiae sacrum
  • Place of Presentation: Berlin,Germany
• [Presentation] New otoendoscopy with catheter for inner ear drug delivery (2008)
  • Author(s): Kanzaki S, Ogawa K
  • Organizer: (poster) the American Academy of Otolaryngology-Head and Neck Surgery Foundation
  • Place of Presentation: Washington Metro Convention Center, USA
• [Presentation] The role of inflammation and apoptosis in sudden sensorineural hearloss (oral presentation) (2008)
  • Author(s): Ogawa K, Kanzaki S, Okamoto Fujioka M, Matsunaga T
  • Organizer: Collegium, Oto-Rhino-Laryngologicum
  • Place of Presentation: Berlin, Germany
• [Presentation] 骨粗軽症モデルマウスにおける耳小骨の検討 (2007)
  • Author(s): 神崎 晶、小 川郁
  • Organizer: 第17回日本耳科学会
  • Place of Presentation: 福岡
  • Year and Date: 2007-10-18
• [Presentation] 突発性難聴患者の末梢血におけるバイオマーカーの検討 (2007)
  • Author(s): 神崎 晶、増田 正次、佐藤 美奈子、藤 秀行、水足 邦雄、鈴木 隆史、大石 直樹、若林 健一郎、井上 泰宏、小 川郁
  • Organizer: 第52回日本聴覚医学会
  • Place of Presentation: 名古屋
  • Year and Date: 2007-10-04
• [Presentation] Preventing auditory bone resorption & gene and cell herapy(invited speaker)招待講演, seminar in Massachusetts Eye and Ear Infirmary Department of Otolaryngology (2007)
  • Author(s): Kanzaki S
  • Organizer: Harvard University
  • Place of Presentation: Boston, USA
  • Year and Date: 2007-09-20
• [Presentation] Otosclerosis : Basic and clinical aspect (2007)
  • Author(s): Ogawa K, Inoue Y, Saito H, Kanzaki S
  • Organizer: (oral), Collegium, Oto-Rhino-Laryngologicum, Amicitiae sacrum
  • Place of Presentation: Seoul,Korea
• [Presentation] Bisphosphonate Therapy Ameliorates Hearing Loss Due to Osteopenia in Mice Lacking Osteoprotegerin (2007)
  • Author(s): Kanzaki S, Ito M, Ogawa K, Matsuo K
  • Organizer: 2007 Association for Research in Otolaryngology MidWinter Meeting, Session D9 : Middle Ear 1
  • Place of Presentation: Denver, Colorado, USA
• [Presentation] New otoendoscopy with catheter for inner ear drug delivery (2007)
  • Author(s): Kanzaki S, Ogawa K
  • Organizer: the American Academy of Otolaryngology- Head and Neck Surgery (poster)
  • Place of Presentation: Washington USA,
• [Book] 家庭の医学、小学館 (2009)
  • Author(s): 神崎晶、外耳炎、外耳道湿疹
  • Publisher: 小川郁編
• [Book] 家庭の医学外耳炎、外耳道湿疹 (2009)
  • Author(s): 神崎晶
  • Publisher: 小学館
• [Book] (全編編集協力者)ステッドマン医学辞書(第6版) (2008)
  • Author(s): 神崎晶
  • Publisher: メジカルビュー社