Study for the treatment with ALDH2 against age-related retinal degeneration.

• Project/Area Number: 19791254
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Ophthalmology
• Research Institution: Tohoku University
• Principal Investigator: SUGANO Eriko Tohoku University, 国際高等研究教育機構, 助教 (70375210)
• Project Period (FY): 2007 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥3,660,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥360,000); Fiscal Year 2008: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2007: ¥2,100,000 (Direct Cost: ¥2,100,000)
• Keywords: 加齢 / 網膜 / 遺伝子

Research Abstract

加齢性網膜においても、β-アミロイドの蓄積が報告されている。本研究ではβ-アミロイドのが蓄積されるアルツハイマーモデルを用いて検討を行った。また、それと関連して報告のある酸化物質4-hydroxynonenal(4-HNE)の蓄積について検討を行った。その結果、網膜でβ-アミロイド及び4-HNEの蓄積を起こす個体が存在したが、アルツハイマーモデルであれば、必ずこれらの蓄積を起こす訳ではなく、またその頻度も低い事がわかった。更に、4-HNEは加齢よりむしろ酸化ストレスに関与していると考えられた。

Research Products

• [Journal Article] Molecular determinants differentiatin photocurrent properties of two channel rhodopsins from Chlamydomonas. (2008)
  • Author(s): Wang H., Sugiyama Y., Hikima T., Sugano E., Tomita H., Takahashi T., Ishizuka T., Yawo H.
  • Journal Title: J Biol Chem 284-9 Pages: 5685-5696
  • Peer Reviewed
• [Journal Article] Brain-derived neurotrophic factor inc reases the phagocytic activity mediated through mitogen-activated protein kina se pathway in cultured iris pigment epithelial cells. (2008)
  • Author(s): Yoshida H., Tomita H., Sugano E., Isago H., Ishiguro S.I., Tamai M
  • Journal Title: Cell Struct Funct 33(1) Pages: 21-6
  • Peer Reviewed
• [Journal Article] Molecular determinants differentiating photocurrent properties of two channelrhodopsins from Chlamydomonas (2008)
  • Author(s): Wang H., Sugiyama Y., Hikima T., Sugano E., Tomita H., Takahashi T., Ishizuka T., Yawo H.
  • Journal Title: J Biol Chem. 284-9 Pages: 5685-5696
  • Peer Reviewed
• [Journal Article] Brain-derived neurotrophic factor increases the phagocytic act ivity mediated through mitogen-activated protein kinase path way in cultured iris pigment epithelial cells (2008)
  • Author(s): Yoshida H., Tomita H., Sugano E., Isago H., Ishiguro S.I., Tamai M
  • Journal Title: Cell Struct Funct. 33(1) Pages: 21-6
  • Peer Reviewed
• [Journal Article] Evaluation of Platinum-Black (Pt-b) Stimulus Electrode Array for Electrical Stimulation to Retinal Cells in Retinal Prosthesis System. (2007)
  • Author(s): Watanabe T., Kobayashi R., Komiya K., Fukushima T., Tomita H., Sugano E., Kurino H., Tanaka T., Tamai M., Koyanagi M
  • Journal Title: Japanese Journal of Applied Physics 46(4B) Pages: 2785-91
  • Peer Reviewed
• [Journal Article] Visual responses in aged dystrophic RCS rats were restored by AAV-mediated channelopsin-2 gene transfer. (2007)
  • Author(s): Hiroshi Tomita, Eriko Sugano, Hiromu Yawo, Toru Ishizuka, Hitomi Isago, Satoko Narikawa, Sebastian Kuler, Makoto Tamai
  • Journal Title: Invest Ophthalmol Vis Sci 48(8) Pages: 3821-6
  • Peer Reviewed
• [Journal Article] Evaluation of Platinum-Black (Pt-b) Stimulus Electrode Array for Electrical Stimulation to Retinal Cells in Retinal Prosthesis System. (2007)
  • Author(s): Watanabe T., Kobayashi R., Komiya K., Fukushima T., Tomita H., Sugano E., Kurino H., Tanaka T., Tamai M., Koyanagi M.
  • Journal Title: Japanese Journal of Applied Physics 46(4B) Pages: 2785-91
  • Peer Reviewed
• [Journal Article] Visual responses in aged dystrophic RCS rats were restored by AAV-mediated channelopsin-2 gene transfer. (2007)
  • Author(s): Hiroshi Tomita, Eriko Sugano, Hiromu Yawo, Toru Ishizuka, Hitomi Isago, Satoko Narikawa, Sebastian Kuler, Makoto Tamai
  • Journal Title: Invest Ophthalmol Vis Sci. 48(8) Pages: 3821-6
  • Peer Reviewed
• [Presentation] Restore Vision Using Channelrhodopsin-2: Dissemination of AAV Vector in Channelrhodopsin-2 transferred rat. (2009)
  • Author(s): Eriko Sugano, Hiroshi Tomita, Hitomi Isago, Teru Hiroi, Makoto Tamai
  • Organizer: Asia-ARVO(アジア目艮科研究会議)
  • Place of Presentation: インド, hyderabad, International Conv. Cent
  • Year and Date: 2009-01-18
• [Presentation] Restore Vision Using Channelrhodopsin-2 : Dissemination of AAV Vector in Channelrhodopsin-2 transferred rat (2009)
  • Author(s): Eriko Sugano, Hiroshi Tomita, Hitomi Isago, Teru Hiroi, Makoto Tamai
  • Organizer: Asia-ARVO(アジア眼科研究会議)
  • Place of Presentation: インド, hyderabad, International Conv. Cent
  • Year and Date: 2009-01-18
• [Presentation] Channelrodopsin-2による遺伝盲ラットの視機能再建-免疫学的検討- (2008)
  • Author(s): 菅野江里子, 富田浩史, 砂金ひとみ, 玉井信
  • Organizer: 臨床眼科学会
  • Place of Presentation: 東京国際フォーラム
  • Year and Date: 2008-10-23
• [Presentation] Channelrodopsin-2による遺伝盲ラットの視機能再建-免疫学的検討- (2008)
  • Author(s): 菅野江里子, 富田浩史, 砂金ひとみ, 玉井信
  • Organizer: 第79回日本動物学会
  • Place of Presentation: 福岡大学七隈キャンパス
  • Year and Date: 2008-09-07
• [Presentation] Channelrodopsin-2による遺伝盲ラットの視機能再建-免疫学的検討- (2008)
  • Author(s): 菅野江里子, 富田浩史, 砂金ひとみ, 玉井信
  • Organizer: 第79回日本動物学会
  • Place of Presentation: 東京国際フォーラム
  • Year and Date: 2008-04-29
• [Presentation] Immune Responses to Adeno Associated Virus Type2 Encoding Channelrhodopsin-2 in Genetically Blind Rat Model for Gene Therapy. (2008)
  • Author(s): E. Sugano, H. Tomita, H. Isago, H. Yawo, T. Ishizuka, M. Tamai
  • Organizer: ARVO(国際眼科研究会議)
  • Place of Presentation: Fortlauderdale, FL, International Conv. Cent
  • Year and Date: 2008-04-29
• [Presentation] Immune Responses to Adeno Associated Virus Type2 Encoding Channelr hodopsin-2 in Genetically Blind Rat Model for Gene Therapy (2008)
  • Author(s): E. Sugano, H. Tomita, H. Isago, H. Yawo, T. Ishizuka, M. Tamai
  • Organizer: ARVO(国際眼科研究会議)
  • Place of Presentation: Fortlauderdale, FL, Inter national Cony. Cent
  • Year and Date: 2008-04-29
• [Presentation] Restoration of visual responses in genetically blind rats using AAV-mediated channelopsin-2 gene transfer (2007)
  • Author(s): Eriko Sugano, Hiroshi Tomita, Hiromu Yawo, 他
  • Organizer: Society for Neuroscience(北米神経科学会)
  • Place of Presentation: Sandiego, CA
  • Year and Date: 2007-11-05
• [Presentation] Improvement of behavioural response in genetica11y blind rat transffered the channelopsin-2 gene. (2007)
  • Author(s): Hiroshi Tomita, Eriko Sugano, Hiromu Yawo, 他
  • Organizer: Society for Neuroscience(北米神経科学会)
  • Place of Presentation: Sandiego, CA
  • Year and Date: 2007-11-05
• [Presentation] チャネルロドプシン-2遺伝子導入による遺伝盲ラットの視機能の回復 (2007)
  • Author(s): 菅野江里子、富田浩史、砂金ひとみ、他
  • Organizer: 日本動物学会
  • Place of Presentation: 弘前大学
  • Year and Date: 2007-09-21
• [Presentation] 遺伝子導入による新しい視機能再建法 (2007)
  • Author(s): 富田浩史、菅野江里子、砂金ひとみ, 他
  • Organizer: 日本動物学会
  • Place of Presentation: 弘前大学
  • Year and Date: 2007-09-20
• [Presentation] 眼の機能にかかわる色素細胞の機能進化 (2007)
  • Author(s): 矢野 由希子・田沼 亮・築地 長治・菅野 江里子・富田 浩史・玉井信・小倉淳・峯田 克彦・池尾一穂・五條堀 孝・石黒 誠一・山本 博章
  • Organizer: 日本進化学会第9回大会
  • Place of Presentation: 京都大学
  • Year and Date: 2007-08-31
• [Presentation] 視機能回復への展望<光感受性遺伝子導入による視覚誘発電位の回復> (2007)
  • Author(s): 富田浩史、菅野江里子、八尾寛, 他
  • Organizer: 国際バイオEXPO
  • Place of Presentation: 東京ビックサイト
  • Year and Date: 2007-06-21
• [Presentation] Stable Expression of Thioredoxins in RPE Cells Increases Cell Viabilities During Oxidative Stress (2007)
  • Author(s): Eriko Sugano, Hiroshi Tomita, Wei Cao, 他
  • Organizer: ARVO(国際眼科研究会議)
  • Place of Presentation: Fortlauderdale, FL
  • Year and Date: 2007-05-10
• [Presentation] Differences of Visually Evoked Potentials Between in Young and Aged RCS Rats Transferred Channelopsin 2 Gene (2007)
  • Author(s): Hiroshi Tomita, Eriko Sugano, Hiromu Yawo, 他
  • Organizer: ARVO(国際眼科研究会議)
  • Place of Presentation: Fortlauderdale, FL
  • Year and Date: 2007-05-09
• [Presentation] Behavioural response in the royal college of surgeons rat was improved by the channelopsin-2 gene transfer (2007)
  • Author(s): Hiroshi Tomita, Eriko Sugano, Hiromu Yawo
  • Organizer: Vision Research Conference, Retinal Degeneration and Gene Therapy (VRES)(網膜変性と遺伝子治療に関する視覚研究会議)
  • Place of Presentation: Fortlauderdale, FL
  • Year and Date: 2007-05-04
• [Presentation] 光障害による視細胞変性と光感受性遺伝子による光反応の回復 (2007)
  • Author(s): 菅野江里子、富田浩史、八尾寛、他
  • Organizer: 第111回日本眼科学会, 生体材料シンポジウム
  • Place of Presentation: 大阪国際会議場
  • Year and Date: 2007-04-19
• [Presentation] 人工網膜に替わる新規視機能再建法の検討 (2007)
  • Author(s): 菅野江里子、富田浩史、八尾寛、他
  • Organizer: 第111回日本眼科学会
  • Place of Presentation: 大阪国際会議場
  • Year and Date: 2007-04-19
• [Presentation] 光感受性遺伝子導入によるRCSラットの視覚誘発電位の回復 (2007)
  • Author(s): 富田浩史、菅野江里子、八尾寛, 他
  • Organizer: 第111回 日本眼科学会
  • Place of Presentation: 大阪国際会議場
  • Year and Date: 2007-04-19
• [Presentation] 人工網膜に替わる新規視機能再建法の検討 (2007)
  • Author(s): 菅野江里子、富田浩史、八尾寛, 他
  • Organizer: 第111回 日本眼科学会
  • Place of Presentation: 大阪国際会議場
  • Year and Date: 2007-04-19
• [Presentation] 光障害による視細胞変性と光感受性遺伝子による光反応の回復 (2007)
  • Author(s): 菅野江里子、富田浩史、八尾寛, 他
  • Organizer: 第111回 日本眼科学会
  • Place of Presentation: 大阪国際会議場
  • Year and Date: 2007-04-19
• [Book] 「チャネルロドプシン」第62巻11号 (2008)
  • Author(s): 富田浩史、菅野江里子、玉井信
  • Publisher: 医学書院臨床眼科 増刊号 網膜硝子体診療update
• [Book] 臨床眼科増刊号網膜硝子体診療update「チャネルロドプシン」 (2008)
  • Author(s): 富田浩史、菅野江里子、玉井信
  • Total Pages: 6
  • Publisher: 医学書院
• [Remarks]
  • URL: http://www.iiare.tohoku.ac.jp/laboratory/researcher.html
• [Remarks]
  • URL: http://www.visual-neuroscience.med.tohoku.ac.jp/index.html
• [Remarks]
  • URL: http://www.iiare.tohoku.ac.jp/laboratory/researcher.html
• [Remarks]
  • URL: http://www.visual-neuroscience.med.tohoku.ac.jp/index.html
• [Remarks]
  • URL: http://www.iiare.tohoku.ac.jp/laboratory/researcher.html#
• [Patent(Industrial Property Rights)] 遺伝子改変による光感受性増大の方法 (2008)
  • Inventor(s): 富田浩史、菅野江里子
  • Industrial Property Rights Holder: 富田浩史、菅野江里子
  • Filing Date: 2008-03-12
• [Patent(Industrial Property Rights)] 遺伝子改変による光感受性増大の方法 (2007)
  • Inventor(s): 富田浩史、菅野江里子
  • Industrial Property Rights Holder: 富田浩史、菅野江里子
  • Filing Date: 2007-12-18
• [Patent(Industrial Property Rights)] 遺伝子導入による視覚機能の再建方法
  • Inventor(s): 富田浩史、玉井信、菅野江里子
  • Industrial Property Rights Holder: 富田浩史、玉井信、菅野江里子